Japan Society for the Promotion of Science:Grants-in-Aid for Scientific Research
Date (from‐to) : 2009 -2011
Author : HANAJIRI Tatsuro; YOSHIMORO Tomomi; MIZUKI Toru; KASHIWAGI Kunihiro
By combining the electrophoresis method and the conventional Coulter method, we previously proposed the Electrophoretic Coulter Method(ECM)- a technique that enables simultaneous analysis of the size and ζvalues of individual specimens. In this study, we validate ECM in ζmeasurement of biological cells by a conventional Dynamic Light Scattering(DLS) to apply ECM to the field of clinical medicine. We use sheep's red blood cells(RBCs) as biological cells. The ζof RBC is varied by adsorption of Immunogloblin G(IgG) to RBC. We are able to obtain the difference between ζs of RBCs and those of RBCs-IgG by using ECM. In addition, we demonstrate thatζs obtained using ECM show a good agreement with those obtained by the conventional DLS and our ECM can distinguish biological cells using the difference ofζs. Furthermore, we propose that SOI(Silicon On Insulator) wafers or SOQ(Silicon On Quartz) wafers should be utilized as platforms for integration of ECM systems combined with electric detectors or optical detectors and sorting systems, and we characterize fundamental characteristics of SOI wafers and SOQ wafers in detail.