2015/02 - 2017/08 Gunma UniversityIntegrative Phisiology, Graduate School of Medicine講師
2009/04 - 2015/01 群馬大学 医学(系)研究科(研究院)助教
2006/04 - 2009/03 National Institute for Physiological Sciences研究員
2006 - 2008 National Institute for Physiological SciencesDepartment of Developmental Physiology特別協力研究員
Association Memberships
THE JAPAN GERIATRICS SOCIETY 日本精神神経学会 前橋市医師会 群馬県医師会 日本医師会 日本生理学会 JAPAN SOCIETY FOR DEMENTIA RESEARCH JAPANESE PSYCHOGERIATRIC SOCIETY 伊香保BSの会(代表) 北米神経科学学会 日本神経科学学会
N. Shimokawa; B. Yousefi; S. Morioka; S. Yamaguchi; A. Ohsawa; H. Hayashi; A. Azuma; H. Mizuno; M. Kasagi; H. Masuda; H. Jingu; S. -I. Furudate; A. Haijima; Y. Takatsuru; T. Iwasaki; M. Umezu; N. Koibuchi
Joint RANZCP/JSPN symposium: ‘Innovations in psychiatric continuing professional development’ Training for “hybrid” psychiatrist ~change the career from basic science to bed-side job~ [Not invited]
Takatsuru Y
RANZCP2019 2019/05 Public symposium
周産期ストレスマウスに見られる初老期認知機能障害. [Not invited]
高鶴裕介
第37回日本認知症学会学術集会 2018/10 Poster presentation
当院診療患者における多剤耐性細菌感染症の動向. [Not invited]
高鶴裕介; 服部徳昭
第34回北関東病院感染対策談話会 2018/08 Oral presentation
脳梗塞回復期におけるハロペリドールの投与は機能回復を遅延させる. [Not invited]
高鶴裕介; 福本幹仁; 天野出月; 三谷和子
第60回日本老年医学会学術集会 2018/06 Poster presentation
周産期ストレスマウスに見られる認知機能障害. [Not invited]
高鶴裕介
第94回日本生理学会 2018/03 Poster presentation
The effect of mild perinatal hypothyroidism in adult female mice behavior. [Not invited]
Khairinisa MA; Takatsuru Y; Amano I; Koibuchi N.
AOTA2016 2017/03 Poster presentation
Effects of perinatal mild hypothyroidism on learning and memory function of adult male offspring [Not invited]
Amano I; Takatsuru Y; Khairinisa MA; Kokubo M; Haijima A; Koibuchi N.
AOTA2016 2017/03 Poster presentation
周産期ストレス暴露により初老期に見られる認知機能低下 [Not invited]
髙鶴裕介; 蓜島旭; 矢島弘之; Khairinisa MA; 天野出月; 鯉淵典之
第94回日本生理学会 2017/03 Poster presentation
胎児期におけるプロラクチン曝露は将来の母性行動発現に重要である [Not invited]
増田真之佑; 西連寺拓; 天野出月; 髙鶴裕介; 金子涼輔; 下川哲昭; 鯉淵典之
第94回日本生理学会 2017/03 Poster presentation
海馬初代培養細胞の発達における甲状腺ホルモンの役割の解明 [Not invited]
矢島弘之; 天野出月; 宮崎航; 髙鶴裕介; 鯉淵典之
第94回日本生理学会 2017/03 Poster presentation
授乳期のパーフルオロオクタンスルホン酸曝露が学習・記憶機能へ及ぼす影響 [Not invited]
蓜島旭; 天野出月; 髙鶴裕介; 鯉淵典之
第94回日本生理学会 2017/03 Poster presentation
Iridium complex probe, BMTPDM1, is useful probe for in vivo imaging of oxygen dynamics. [Not invited]
Takatsuru Y; Koibuchi N; Yoshihara T; Tobita M.
3rd International Symposium of Gunma University Medical Innovation and 8th International Conference on Advanced Micro-Device Engineering (GUMI&AMDE2016) 2016/12 Poster presentation
Mild perinatal thyroid hormone insufficiency induced learning and memory disorders in adult mice. [Not invited]
Amano I; Takatsuru Y; Khairinisa MA; Kokubo M; Haijima A; Koibuchi N
4th Bandung International Biomolecular Medicine Conference 2016/10 Poster presentation
Cognitive function impairment in mouse model of mild perinatal hypothyroidism [Not invited]
Khairinisa MA; Takatsuru Y; Amano I; Koibuchi N.
4th Bandung International Biomolecular Medicine Conference 2016/10 Poster presentation
多光子励起レーザー顕微鏡FVMPE-RSを用いたin vivo imagingの実例 [Not invited]
高鶴裕介; 金子涼輔; 鯉淵典之
第63回北関東医学会総会 2016/09 Poster presentation
Aripiprazole improves the remodeling of neuronal circuit for functional recovery after stroke in somatosensory cortex [Not invited]
Augmentation of thyroid hormone receptor-mediated transcription and thyroid hormone-induced cerebellar morphogenesis by genistein and daidzein [Not invited]
In vivo deep, red color imaging using advanced multi-photon laser microscopy (FVMPE-RS, Olympus) [Not invited]
Takatsuru Y; Kaneko R; Kominato Y; Noriyuki K
International Society of Radiation Neurobiology 5th Conference 2015/02 Poster presentation
In vivo imaging of oxygen dynamics using Iridium complex probe [Not invited]
Takatsuru Y; Koibuchi N; Yoshihara T; Tobita M.
1st International Symposium of Gunma University Medical Innovation and 6th International Conference on Advanced Micro-Device Engineering (GUMI&AMDE2014) 2014/12 Poster presentation
The stress of mother-separation cause significant effects for development of dendritic spines in layer V pyramidal neuron in motor cortex. [Not invited]
Yusuke Takatsuru; Junichi Nabekura
The 31th Annual meeting of the Japan Neuroscience Society. 2008/07 Poster presentation
Functional roles of glutamate transporters in cerebellar Purkinje cell synapses [Not invited]
Iino, M; Takayasu, Y; Takatsuru, Y; Ozawa, S
The 85th Annual meeting of the Physiological Society of Japan 2008/03 Poster presentation
Regulation of excitatory synaptic transmisson by glial glutamate transporters [Not invited]
Takatsuru, Y; Takayasu, Y; Iino, M; Ozawa, S
The 29th Annual meeting of the Japan Neuroscience Society 2006/07 Poster presentation
Regulation of excitatory synaptic transmission by glial glutamate transporters [Not invited]
Takatsuru, Y; Takayasu, Y; Iino, M; Ozawa, S
The 83nd Annual meeting of the Physiological Society of Japan. 2006/03 Nominated symposium
glutamate transporter, GLT-1, in climbing fibber-Purkinje cell synapses in the cerebellum. [Not invited]
Yusuke TakatsuruTakatsuru, Y; Takayasu, Y; Nikkuni, O; Iino, M; Ozawa, S
The 82nd Annual meeting of the Physiological Society of Japan. 2005/05 Poster presentation
C-terminal cytoplasmic domain for expression of functional NMDA receptors in PC12 cells [Not invited]
HAIJIMA Asahi; SHIMOKAWA Noriaki; TAKATSURU Yusuke; AMANO Izuki; LESMANA Ronny; KOIBUCHI Noriyuki Annual Meeting of the Japanese Society of Toxicology 40- (0) 2002100 -2002100 2013
Japan Society for the Promotion of Science:Grants-in-Aid for Scientific Research
Date (from‐to) : 2020/09 -2022/03
Author : Takatsuru Yusuke
In order to study the relationship between food preference and brain function, we conducted experiments on fMRI and fNIRS for healthy volunteers. The sensitivity of the smell is more significant in women, and the function decreases in both sexes in age dependence. FMRI experiments show that the sex difference and age-dependent functional changes are particularly involved in the functional connectivity of the frontal medial cortex and angular gyrus. In addition, a study using fNIRS suggested that there may be differences in frontal lobe function at the time of eating depending on the living environment (whether it is with the family or alone) and the preference of the usual food (whether they like the salty taste or the mild taste). In the future, we plan to expand the number of subjects to continue the fNIRS experiment.
周産期ストレスに起因する思春期精神疾患発症メカニズムの解明
Japan Society for the Promotion of Science:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
Date (from‐to) : 2017/04 -2018/03
Author : 高鶴 裕介
周産期のストレスが脳の正常な発達を妨げ、うつ病を代表とする精神疾患の罹患率を上げることが知られている。このような変化は動物実験においても再現されているが、その背景にあるメカニズムは未解明である。 研究代表者らはこれまで、母仔乖離マウス(Maternal deprivationマウス; MDマウス)を用いた研究を通じて、周産期ストレスがグルタミン酸恒常性の破綻やシナプスの不安定化を引き起すことを示してきた(Takatsuru et al., 2009, Toya et al., 2014ほか)。その後の研究で、初老期のMDマウスでは認知力が著名に低下していることも発見した。この研究では若年期の認知能力は正常であったが、これまでの知見から、MDマウスは特に性成熟前期(6-8週齢)にシナプスの脆弱性が顕著であることを報告しており(Takatsuru et al., 2009)、認知機能に関してはさらなる研究の継続が必要であると考えている。そこで本計画では、性成熟前期のMDマウスを様々な環境で飼育し、それに伴う認知機能の変化を研究することで、周産期ストレスが思春期の脳機能およびストレス脆弱性の形成に寄与するメカイズムを解明することを計画した。
本研究の結果、周産期ストレスを負荷した後のマウスを個別に飼育した場合と、集団で飼育した場合とで、その後の学習行動試験(タッチパネルオペラント装置を用いた視覚弁別課題)の結果に差が見られる可能性が示唆された。この原因について精査することを計画したが、研究代表者の異動・科研費申請資格喪失に伴い、研究終了となった。
Japan Society for the Promotion of Science:Grants-in-Aid for Scientific Research Grant-in-Aid for Research Activity start-up
Date (from‐to) : 2015/08 -2017/03
Author : Hanamura Kenji; TAKATSURU YUSUKE
In vivo imaging by using two-photon laser microscopy under deep anesthesia revealed dendritic spine morphology of pyramidal neurons in the cerebral cortex of transgenic mice expressing GFP under the control of Thy1-promoter. Analysis of heterozygotes of knockout mice deficient in drebrin A-specific exon or all drebrin isoforms tended to show some changes in turnover rate of dendritic spines compared with wild-type mice. The amount of NMDA receptors in synaptosomes were detected by western blotting.
In this study, we observed the role of astrocyte on remodeling for functional compensation in contralateral hemisphere of focal stroke. We found that dopamine is one of the strong candidate for contributing the functional remodeling/recovery after focal stroke. We also found that repetitive application of Aripiprazole, one of the partial agonist for dopamine receptor, enhances the remodeling of neuronal circuit in intact hemisphere after focal stroke. Since the Aripiprazole enhances the activity of astrocyte if it repetitively applied, our study suggested that application of aripiprazole enhances the neuronal remodeling after focal stroke via activation of dopaminergic systems.
Japan Society for the Promotion of Science:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
Date (from‐to) : 2013/04 -2017/03
Author : Koibuchi Noriyuki; NABWKURA Junichi
This study was designed to examine the effect of the alteration of nuclear receptor-mediated gene expression on brain development. Using in vivo preparation, we found that congenital hypothyroid mouse and rat showed cerebellar ataxia. Electrophysiological study showed that such alteration may be induced by an aberrant synaptic function between parallel fiber and Purkinje cell. Perinatal PCB treatment induced the similar phenotype. Using in vitro preparation, we established the primary culture system of the hippocampus to study the effect of thyroid hormone. In addition, we examined the affect of gadolinium-based contrast agents (GBCA) on thyroid hormone action and found that linear GBCA disrupted thyroid hormone receptor action and thyroid hormone-mediated cerebellar dendritogenesis of Purkinje cell.
Japan Society for the Promotion of Science:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
Date (from‐to) : 2011/04 -2013/03
Author : 高鶴 裕介
母子乖離操作により慢性ストレスを付加したマウスを成体の時点で研究した結果、以下の知見を得た。
雄の母子乖離マウスにおいて、(1)母子乖離マウスでは体性感覚野における神経活動が亢進しており、それに相関して知覚閾値の低下がみられた。(2)同部位ではグルタミン酸の過剰放出が起こっていた。(3)ホームケージにいるときでもストレスホルモン(コルチコステロン)の分泌が増加していた。(4)急性ストレスの追加負荷により、通常みられるコルチコステロンの一過性上昇が、母子乖離マウスでは消失していた。一方、同時に計測したグルタミン酸の濃度は、母子乖離マウスで顕著に増加していた。(5)急性ストレス負荷後、対照群に比べ一部のグルタミン酸受容体の発現が過剰増加していた。(6)同マウスをin vivo imaging法により生きたまま観察すると、マイクログリアの動態が変化していることが分かった。以上の結果は2報の論文として執筆中である。
また、雌の母子乖離マウスにおいて(1)母子乖離マウスは妊娠・出産に至る割合が低下し、かつ、仔育てを失敗する(育児放棄により仔が死亡する)割合が増加していた。(2)母子乖離マウスを母親にもつ仔では、体重が減少傾向にあり、血中コルチコステロン濃度が有意に増加していた。(3)母子乖離マウスを母親にもつ仔の行動パターンに異常が見られた。(4)(1)の知見は世代間で伝搬する傾向があり、母子乖離マウスの娘や孫でも妊娠・出産率の低下や育児放棄率の増加がみられた。以上の結果は1報の論文として執筆中である。
Above-referenced theme was analyzed using thyroid hormone (TH) . TH receptor (TR) system as a model system. Congenital hypothyroid rat showedhyperactivity under novel environment. We found that the effect is induced by disruption of dopamine transport due to a decreased tubulin expression, which is regulated by TH.In primary cerebellar culture, TH induced an increase in dendrite and neurite elongation,which was inhibited by environmental chemicals such as polybrominated diphenylether(PBDE). PBDE did not alter TR-cofactor binding. Instead, it dissociated TR from DNA.
Japan Society for the Promotion of Science:Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)
Date (from‐to) : 2009 -2010
Author : TAKATSURU Yusuke
Remodeling and/or synapse plasticity is increased at 1^ week after stroke in intact hemisphere. In contrast, recovery of general behavior is delay compared with those in simple somatosensory function. During the remodeling, glutamine, glysine and dopamine seems to be play an important role. Application of haloperidol at 2-4 days after stroke prolongs the recovery.
The neuronal K(+)-Cl(-) cotransporter (KCC2) is a membrane transport protein that extrudes Cl(-) from neurons and helps maintain low intracellular [Cl(-)] and hyperpolarizing GABAergic synaptic potentials. Depolarizing gamma-aminobutyric acid (GABA) responses in neonatal neurons and following various forms of neuronal injury are associated with reduced levels of KCC2 expression. Mutation to the putative tyrosine phosphorylation site within the long intracellular carboxyl terminus of KCC2(Y1087D) or application of the tyrosine kinase inhibitor genistein shifted the GABA reversal potential (E(GABA)) to more depolarized values, indicating reduced KCC2 function. This was associated with a change in the expression pattern of KCC2 from a punctate distribution to a more uniform distribution, suggesting that functional tyrosine-phosphorylated KCC2 forms clusters in restricted membrane domains. A tyrosine phosphatase inhibitor increased the proportion of KCC2 associated with lipid rafts membrane domains. These results indicate that direct tyrosine phosphorylation of KCC2 results in membrane clusters and functional transport activity. Oxidative stress resulted in a rapid dephosphorylation of KCC2 that preceded the decreases in KCC2 protein or mRNA expression. Dephosphorylation of KCC2 is correlated with a reduction of transport activity and a decrease in [Cl-]i, as well as a reduction in KCC2 surface expression. Manipulation of KCC2 tyrosine phosphorylation resulted in altered neuronal viability in response to in vitro oxidative stress. We propose that neuronal stress induces a rapid loss of tyrosine phosphorylation of KCC2 that results in translocation of the protein and functional loss of transport activity.