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Kazuya Shimizu

    Department of Life Sciences　Professor
    Research Institute of Industrial Technology　Researcher
    Course of Life Sciences　Professor
Contact： k_shimizutoyo.jp
Last Updated :2025/04/20

Researcher Information

Degree

  • Ph.D（2009/03 University of Tsukuba）

Research funding number

  • 10581613

J-Global ID

Profile

  • The lab's motto is "Dreams are hope, hope is courage, and courage makes dreams come true". The motto of our laboratory is "Dreams are hopes, hopes are courage, and courage makes dreams come true".
    I studied engineering at university, thinking that the technology gained through the creation and development of the space industry could be the key to solving problems such as food, population and energy. However, when I considered going to graduate school, I focused on 'bio-use in life-support systems' and 'sustainable use of freshwater' and began research into bio-industrial applications, which continues to this day.
    The sustainable use of fresh water has become an extremely important issue in regions around the world, though not necessarily in space stations. My goal is to realise "safe and secure water use by anyone, anytime, anywhere". To achieve this goal, my research focuses on the use of microorganisms (especially bacteria and protozoa) that contribute to the sustainable use of freshwater, and on the microbial ecosystem of water bodies.

Research Interests

  • Aquatic Environment in tropical region   Antibiotics   protozoa   micro-animal   Antimicrobial agents   Hydrospheric phenomenon   geosmin   Cyanotoxin   Biofilm treament   水環境修復   Thailand   Cyanobacteria   Microcystis   カビ臭物質   ミクロシスティン   養魚池   水圏生態学   処理技術   富栄養化湖沼   Aquatic environment   スクリーニング   Quorum-sensing   cell-cell communication   musty and earthy odor   Biofilm   生物膜処理   Water treatment   microcystin degradation   microcystin synthesis   藍藻由来毒性物質   nodularin   signal transduction   microcystin-degrading bacteria   microcystin   Harmful algal bloom   

Research Areas

  • Social infrastructure (civil Engineering, architecture, disaster prevention) / Civil engineering (environmental systems)
  • Environmental science/Agricultural science / Environmental policy and society
  • Environmental science/Agricultural science / Environmental impact assessment
  • Environmental science/Agricultural science / Environmental materials/recycling technology
  • Environmental science/Agricultural science / Environmental load reduction/restoration technology
  • Environmental science/Agricultural science / Landscape science
  • Environmental science/Agricultural science / Environmental agriculture
  • Environmental science/Agricultural science / Environmental dynamics

Academic & Professional Experience

  • 2022/04 - Today  Toyo UniversityFaculty of Life SciencesProfessor
  • 2021/11 - 2022/03  University of TsukubaFaculty of Life and Environmental SciencesAssociate Professor
  • 2021/01 - 2021/11  University of TsukubaOffice of Global InitiativesAssociate Professor
  • 2017/03 - 2020/12  University of Tsukuba生命環境系Associate Professor
  • 2016/04 - 2017/02  Toyo UniversityDepartment of Life SciencesAssociate Professor
  • 2013/04 - 2016/03  Toyo UniversityDepartment of Life SciencesSenior Assistant Professor
  • 2010/04 - 2013/03  Toyo UniversityFaculty of Life SciencesAssistant Professor
  • 2009/10 - 2010/03  Sun Care Fuels CorporationResearcher
  • 2009/04 - 2010/03  Graduate School of Life and Environmental Sciences, University of TsukubaPosdoc
  • 2003/04 - 2004/03  Nihon u\UniversityResearcher

Education

  • 2006/04 - 2009/03  University of Tsukuba  Graduate School of Life and Environmental Sciences  生命産業科学専攻
  • 2004/04 - 2006/03  University of Tsukuba  バイオシステム研究科  バイオシステム専攻
  • 1999/04 - 2003/03  Nihon University  理工学部  航空宇宙工学科

Association Memberships

  • JAPAN WATER WORKS ASSOCIATION   日本水処理生物学会   日本水環境学会   International Society for Microbial Ecology   日本農芸化学会   

Published Papers

Books etc

  • The effects of administrated low concentrations of microcystin, a cyanobacterial toxin, on the liver and colon
    清水英寿; 古東義仁; 河原秀明; 吉清恵介; 岡野邦宏; 杉浦則夫; 清水和哉 2021
  • Environment and Biotechnology; Chapter 12: Degradation of some secondary metabolites in aquatic environment.
    清水 和哉; Sugiura N; Xue Q; Li J; Shimizu K; Utsumi M; Zhang Z; Sakhakar R. K LAP Lambert Academic Publishing 2012/08 
    水環境中の藍藻類は水利用障害を引き起こす様々な二次代謝産物を産生する。本章では、それら二次代謝産物を微生物がどの様に分解しているのかを詳細に述べた。

Conference Activities & Talks

  • Survival of Antibiotic-resistant Pathogens in Dairy Manure on Psychrophilic Anaerobic Digestion
    田中優花; 井原一高; 岩崎匡洋; MOHAMED Farghali; YOU Jingyi; 吉田弦; ANDRIAMANOHIARISOAMANANA Fetra J.; 清水和哉; 間世田英明
    日本水環境学会年会講演集  2024
  • 低温メタン発酵における乳牛糞尿に残留する抗生物質耐性病原菌の消長評価
    田中優花; YOU Jingyi; 吉田弦; 岩崎匡洋; FARGHALI Mohamed; 井原一高; ANDRIAMANOHIARISOAMANANA Fetra J.; 清水和哉; 間世田英明
    農業施設  2024
  • Current status and directions of research and technological development of genetic analysis for environmental risk assessment of water use affecting cyanobacteria and other organisms.
    清水和哉
    日本水環境学会シンポジウム講演集  2024
  • Effects of pH and temperature on microcystis group strength
    橋井萌絵; SAENCHAN Somsri; PHONGSAWATDY Chanpheng; 清水和哉; 板山朋聡
    日本水環境学会年会講演集  2024
  • Chronic low-concentration exposure to microcystin-LR derived from the blue-green algae leads to upregulation of MCP-1 expression through activation of JNK in the colon.
    古東義仁; 河原秀明; 蔵田航一; 吉清恵介; 杉浦則夫; 清水和哉; 清水英寿
    日本農芸化学会大会講演要旨集(Web)  2021
  • ラットに対するミクロシスチンの低濃度曝露は結腸においてMCP-1の発現増加を導く
    古東義仁; 河原秀明; 蔵田航一; 吉清恵介; 吉清恵介; 杉浦則夫; 清水和哉; 清水英寿; 清水英寿
    日本農芸化学会中四国支部講演会講演要旨集(Web)  2021
  • アオコ由来毒素ミクロシスチン-LRはラットにおいて腸線維化を誘導する
    古東義仁; 堀将太; 石塚敏; 杉浦則夫; 清水和哉; 清水英寿
    日本分子生物学会年会プログラム・要旨集(Web)  2020
  • 清水英寿; 河原秀明; 蔵田航一; 湯浅佳奈; 渡辺優太; 堀将太; 石塚敏; 岡野邦宏; 吉清恵介; 山本達之; 西村浩二; 地阪光生; 横田一成; 杉浦則夫; 清水和哉
    日本農芸化学会大会講演要旨集(Web)  2019/03
  • アオコ由来毒素ミクロシスチンの発癌プロモーターとしての作用メカニズムの解析
    渡辺優太; 岡野邦宏; 杉浦則夫; 清水和哉; 清水英寿
    日本分子生物学会年会プログラム・要旨集(Web)  2019
  • 熱帯及び温帯水域由来の藍藻類捕食者の比較
    柳谷将; 板山朋聡; 岩見徳雄; 間世田英明; 岩本浩二; 原啓文; 雷中方; 内海真生; 張振亜; 角野立夫; 清水和哉
    日本水処理生物学会誌 別巻  2019
  • 清水和哉; 柳谷将; 住田透; 板山朋聡; 岩見徳雄; 岡野邦宏; 間世田英明; 雷中方; 内海真生; 角野立夫; 張振亜; 杉浦則夫
    日本水処理生物学会誌 別巻  2018/10
  • 清水和哉; 柳谷将; 岡野邦宏; 間世田英明; 内海真生; 雷中方; 張振亜; 杉浦則夫
    環境バイオテクノロジー学会大会プログラム講演要旨集  2018/06
  • 有毒藍藻類産生毒Microcystinのいさはや新池堤防内外の底泥生息微生物による分解特性と機能評価
    稲森悠平; 稲森隆平; DONG Zhangsen; 類家翔; ZHANG Zhenya; LEI Zhongfang; 清水和哉; 林紀男; 鈴木理恵
    日本水環境学会年会講演集  2018
  • 藍藻毒が藍藻類捕食者に及ぼす影響とその適応機構の解明
    柳谷将; 岩澤俊平; 板山朋聡; LEI Zhongfang; 内海真生; ZHANG Zhenya; 角野立夫; 清水和哉
    日本水環境学会年会講演集  2018
  • 有毒藍藻類産生毒Microcystin類を含有する農業用灌漑用水の農作物への環境リスク評価手法の検討
    類家翔; 稲森隆平; 稲森悠平; 清水和哉; LEI Zhongfang; ZHANG Zhenya
    日本水環境学会年会講演集  2018
  • 古田土実和; 新井美智子; 柳谷将; 岡野邦宏; 内海真生; ZHANG Zhenya; 杉浦則夫; 杉浦則夫; 間世田英明; 間世田英明; 清水和哉
    日本水環境学会年会講演集  2017/03
  • 清水和哉; 岡野邦宏; 鈴木眞菜; 天野(佐藤)千恵; HAO Liting; ZHANG Zhenya; 井原一高; 間世田英明
    日本水環境学会年会講演集  2017/03
  • 有毒藍藻類産生毒Microcystin類の異なる畑土壌成分としての赤玉土および黒ボク土における吸着に及ぼす特性解析
    類家翔; 稲森隆平; 清水和哉; 雷中方; 張振亜; 稲森悠平; 稲森悠平
    日本水処理生物学会誌 別巻  2017
  • 古田土実和; 新井美智子; 黒古成美; 岡野邦宏; 内海真生; 張振亜; 杉浦則夫; 杉浦則夫; 間世田英明; 間世田英明; 清水和哉
    日本水処理生物学会誌 別巻  2016/10
  • 鈴木眞菜; 岡野邦宏; 天野(佐藤)千恵; 天野(佐藤; 千恵; 井原一高; 間世田英明; 間世田英明; 清水和哉
    日本水処理生物学会誌 別巻  2016/10
  • MlrA, the essential enzyme for microcystins biodegradation  [Not invited]
    Miwa Kodato; Michiko Arai; Narumi Kurogo; Kunihiro Okano; Zhenya Zhang; Motoo Utsumi; Norio Sugiura; Hideaki Maseda
    16th International Symposium on Microbial Ecology (ISME16),  2016/08  Poster presentation
  • Removal of cyanobacteria and cyanotoxin using micro-animal and co-existing bacteria in a bioreactor  [Not invited]
    Kazuya Shimizu; Kunihiro Okano; Hiroyuki Kinohira; Hideaki Maseda; Norio Iwami; Tomoaki Itayama; Zhenya Zhang; Motoo Utsumi; Norio Sugiura
    16th International Symposium on Microbial Ecology (ISME16),  2016/08  Poster presentation
  • 黒古成美; 岡野邦宏; 内海真生; 杉浦則夫; 間世田英明; 清水和哉
    日本水環境学会年会講演集  2016/03
  • 五十嵐千聡; 岡野邦宏; 綱取泰広; 天野(佐藤)千恵; 間世田英明; 清水和哉
    日本水環境学会年会講演集  2016/03
  • タイ養魚池における有毒藍藻の増殖要因の解析
    板山朋聡; XIA Dong; 清水和哉; 岩見徳雄; 間世田英明; WHANGCHAI Niwooti; PRAPHRUTE Reunkaew; GUTIERREZ Redel; 杉浦則夫
    日本水環境学会年会講演集  2016
  • Aquaculture fish ponds as experimental ponds for study on toxigenic cyanobacteria in Thailand  [Not invited]
    Tomoaki Itayama; Kazuya Shimizu; Redel Gutierrez; Ruenkeaw Praphrute; Dong Xia; Khomasan Ruangdet; Norio Iwami; Yang Gu; Wang Pengfei; Norio Sugiura
    Aquaculture fish ponds as experimental ponds for study on toxigenic cyanobacteria in Thailand  2014  Oral presentation
  • Problems of water supply relevant to Global Warming  [Not invited]
    清水 和哉; Sugiura Norio; Itayama Tomoaki; Iwami Norio; Maseda Hideaki; Shimizu Kazuya
    The Fourth International Fisheries Conference 2011  2012/12 
    Keynote Presentation
  • Different sensitivity of cyanobacteria and protest by silver nanoparticle  [Not invited]
    清水 和哉; Kanazawa Ayako; Nagasaka Seiji; Nakagame Yuya; Ariyoshi Tadashi; Takei Hiroyuki; Kashiwada Shosaku; Shimizu Kazuya
    6th SETAC World Congress/SETAC Europe 22nd Annual Meeting  2012/05
  • Inhibition on Microcystis aeruginosa growth and microcystins production in oxygen production system using a new electrolysis cell  [Not invited]
    清水 和哉; Gao Y; Shimizu K; Utsumi M; Sugiura N
    第46回日本水環境学会年会  2012/03
  • 生物膜法におけるmicrocystin分解および分解酵素遺伝子の動態解析  [Not invited]
    清水 和哉; 赤迫春菜; 清水和哉; 李潔明; 内海真生; 杉浦則夫
    第46回日本水環境学会年会  2012/03
  • 日本とタイ水域のおけるmicrocystinの合成および分解に関与する遺伝子（mcy, mlr）の比較解析  [Not invited]
    清水 和哉; 古澤文章; 板山朋聡; 内海真生; 清水和哉; 杉浦則夫
    第46回日本水環境学会年会  2012/03
  • 電解酸素曝気が藻類の増殖に及ぼす影響評価  [Not invited]
    清水 和哉; 崔海龍; 高宇; 清水和哉; 内海真生; 杉浦則夫
    第46回日本水環境学会年会  2012/03
  • Degradation of Microcystis cell using Effective Microfauna in Fish pond.  [Not invited]
    清水 和哉; Shioya Kazune; Itayama Tomoaki; Whangchai Niwooti; Fakrajang Siraprapa; Kannika Korntip; Kuwabara Takashi; Sugiura Norio; Shimizu Kazuya; Iwami Norio
    The Fourth International Fisheries Conference 2011  2011/12 
    Oral Presentation
  • Dynamics of microcystin production mcy gene and microcystin degradation mlr gene.  [Not invited]
    清水 和哉; Furusawa Fumiaki; Shimizu Kazuya; Whangchai Niwooti; Itayama Tomoaki; Utsumi Motoo; Sugiura Norio
    The Fourth International Fisheries Conference 2011  2011/12 
    Oral Presentation
  • Water purification by bio-fence system.  [Not invited]
    清水 和哉; Itayama Tomoaki; Iwami Norio; Bi Xue-Ying; Xia Dong; Shimizu Kazuya; Sugiura Norio
    The Fourth International Fisheries Conference 2011  2011/12 
    Invited Speaker
  • Determination of the microcystin-LR degrading genes, mlrAs on biofilm in water purification plant.  [Not invited]
    清水 和哉; Shimizu Kazuya; Maseda Hideaki; Okano Kunihiro; Hiratsuka Takumi; Jimbo Yusuke; Xue Qiang; Itayama Tomoaki; Utsumi Motoo; Zhang Zhenya; Sugiura Norio
    The Fourth International Fisheries Conference 2011  2011/12 
    Invited Speaker
  • ミクロシスチン分解関連タンパク質MlrBの局在と機能解析  [Not invited]
    清水 和哉; 小林宏聡; 清水和哉; 白井昭博; 大政健史; 杉浦則夫; 間世田英明
    日本水処理生物学会 第48回大会  2011/11
  • Application of a new electrolysis cell to toxic cyanobacteria inhibition in water environment  [Not invited]
    清水 和哉; Gao Y; Utsumi M; Shimizu K; Sugiura N
    日本水処理生物学会 第48回大会  2011/11
  • Influence of environmental factors on musty odor production by Streptomyces coelicolor A3(2)  [Not invited]
    清水 和哉; Hikida T; Shimizu K; Utsumi M; Sugiura N
    14th World Lake Conference  2011/11
  • 電解酸素曝気環境下での藻類の増殖特性に関する影響評価  [Not invited]
    清水 和哉; 崔海龍; 高宇; 清水和哉; 内海真生; 杉浦則夫
    日本水処理生物学会 第48回大会  2011/11
  • 生物学的処理法におけるmicrocystin分解活性および分解酵素遺伝子の挙動解析  [Not invited]
    清水 和哉; 赤迫春菜; 清水和哉; 李潔明; 内海真生; 杉浦則夫
    日本水処理生物学会 第48回大会  2011/11
  • 放線菌のかび臭発生に及ぼす各種因子の影響評価  [Not invited]
    清水 和哉; 疋田敏勝; 梅田哲弥; 清水和哉; 内海真生; 杉浦則夫
    日本水処理生物学会 第48回大会  2011/11
  • 国内水域とタイ北部水域のmicrocystin産生能の比較解析  [Not invited]
    清水 和哉
    日本水処理生物学会 第48回大会  2011/11
  • 有毒藍藻産生物質microcystin分解細菌の分解機構の解析  [Not invited]
    清水 和哉; 清水和哉; 間世田英明; 岡野邦宏; 内海真生; 張振亜; 杉浦則夫
    日本農芸化学会関東支部2011年度大会  2011/10
  • Expression of microcystin degradative activity in microcystin-degrading bacterium  [Not invited]
    清水 和哉; Shimizu Kazuya; Maseda Hideaki; Okano Kunihiro; Itayama Tomoaki; Utsumi Motoo; Zhang Zhenya; Sugiura Norio
    International Union of Microbiological Societies 2011 Congress  2011/09
  • Study of biological microcystin degradation at biological treatment facility in a water purification plant, Japan  [Not invited]
    清水 和哉; Akasako Haruna; Shimizu Kazuya; Li Jieming; Utsumi Motoo; Sugiura Norio
    Doctoral Forum of China and the 4th China-Japan Graduate Student Forum  2011/09
  • Characterization of MlrB involved in degradation of cyanobacterial toxin microcystin LR  [Not invited]
    清水 和哉; Kobayashi H; Shimizu K; Zhang Z; Sugiura N; Omasa T; Maseda H
    Doctoral Forum of China and the 4th China - Japan Graduate Student Forum  2011/09
  • 水環境生態系に及ぼす電解酸素の影響評価  [Not invited]
    清水 和哉; 大森啓; 高宇; 崔海龍; 清水和哉; 内海真生; 杉浦則夫
    第45回日本水環境学会  2011/03
  • 生物処理槽におけるmicrocystin分解能および分解酵素遺伝子の挙動解析  [Not invited]
    清水 和哉; 赤迫春菜; 清水和哉; 李潔明; 内海真生; 杉浦則夫
    第45回日本水環境学会  2011/03
  • Microcystin分解産物の特性解析  [Not invited]
    清水 和哉; 清水和哉; 星麻里恵; 伊藤聡史; 間世田英明; 岡野邦宏; 陳栄志; 内海真生; 張振亜; 杉浦則夫
    第45回日本水環境学会  2011/03
  • Application of oxygen productive electrode (OPE) to the toxic cyanobacteria inhibition in water trea  [Not invited]
    清水 和哉; Gao Y; Xue Q; Omori K; Shimizu K; Utsumi M; Sugiura N
    第45回日本水環境学会  2011/03
  • Microcystin分解産物の特性解析,  [Not invited]
    清水 和哉; 清水和哉; 星麻里恵; 伊藤聡; 間世田英明; 岡野邦宏; 陳栄志; 内海真生; 張振亜; 杉浦則夫
    第47回日本水処理生物学会  2010/11
  • 閉鎖性水域におけるかび臭産放線菌の動態とかび臭産生特性に関する研究  [Not invited]
    清水 和哉; 金蓮美; 沼尻伸; 北見康子; 清水和哉; 内海真生; 杉浦則夫
    第47回日本水処理生物学会  2010/11
  • Finding of Microcystin-degrading Bacterium and Elucidation of Its Degradation Mechanism  [Not invited]
    清水 和哉; Shimizu K; Okano K; Maseda H; Kawauchi Y; Utsumi M; Zhang Z; Sugiura N
    13th World Lake Conference  2010/11
  • 放線菌のジェオスミン産生に及ぼす光の影響  [Not invited]
    清水 和哉; 梅田哲也; 清水和哉; 内海真生; 杉浦則夫
    第47回日本水処理生物学会  2010/11
  • Removal characteristics of musty odor geosmin" by biofiltration using various carriers"  [Not invited]
    清水 和哉; Okei I; Shimizu K; Xue Q; Sato C; Utsumi M; Zhang Z; Sugiura N
    13th World Lake Conference  2010/11
  • Biodegradation of microcystins by the bacterial communities symbiotic with microflagellate Monas gut  [Not invited]
    清水 和哉; Li J; Shimizu K; Utsumi M; Sugiura N
    第47回日本水処理生物学会  2010/11
  • Application of oxygen productive electrode (OPE) in water treatment  [Not invited]
    清水 和哉; Gao Y; Shimizu K; Omori K; Xue Q. Utsumi M; Sugiura N
    第47回日本水処理生物学会  2010/11
  • Isolation and identification of novel geosmin-degrading Bacteria  [Not invited]
    清水 和哉; Xue Q; Chen G; Shimizu K; Sakharkar K. M; Utsumi M; Zhang. Z; Sugiura N
    第47回日本水処理生物学会  2010/11
  • 電解酸素発生装置を用いた純酸素曝気による水質改善手法の検討  [Not invited]
    清水 和哉; 大森啓; 清水和哉; 高宇; 内海真生; 杉浦則夫
    第47回日本水処理生物学会  2010/11
  • 飢餓環境が放線菌のかび臭物質産生に及ぼす影響評価  [Not invited]
    清水 和哉; 疋田敏勝; 梅田哲弥; 清水和哉; 内海真生; 杉浦則夫
    第47回日本水処理生物学会  2010/11
  • 放線菌のかび臭物質産生能に影響を与える因子の解析  [Not invited]
    清水 和哉; 梅田哲弥; 清水和哉; 内海真生; 杉浦則夫
    日本農芸化学会2010年度大会  2010/03
  • Effect of cyclic microcystin and its degradation products on transcription of the gene encoding micr  [Not invited]
    清水 和哉; Shimizu K; Okano K; Amemiya T; Maseda H; Utsumi M; Sugiura N
    International Symposium Microbial Interactions Leading to Novel Biological Functions  2010/01
  • Removal characteristics of musty odor "geosmin" by biofiltration using various carriers.  [Not invited]
    清水 和哉; Okei Ichiro; Shimizu Kazuya; Xue Qiang; Sato Chie; Utsumi Motoo; Zhang Zhenya; Sugiura Norio
    International Symposium Microorganisms and Sociality: Intricate Multi-Level Interactions  2009/12 
    Poster Presentation
  • 藍藻由来毒性物質microcystin分解酵素遺伝子の機能解析  [Not invited]
    清水 和哉; 清水和哉; 間世田英明; 岡野邦宏; 内海真生; 杉浦則夫
    第46回日本水処理生物学会  2009/11
  • 藍藻由来毒性物質microcystin分解酵素遺伝子の機能解析  [Not invited]
    清水和哉; 間世田英明; 岡野邦宏; 内海真生; 杉浦則夫
    第46回 日本水処理生物学会  2009/11  Oral presentation
  • ミクロシスティン高分解菌の創製  [Not invited]
    小林弘明; 清水和哉; 岡野邦宏; 高麗寛紀; 小林達彦; 杉浦則夫; 間世田英明
    第46回 日本水処理生物学会  2009/11  Oral presentation
  • Finding of Microcystin-degrading bacterium and Elucidation of Its Degradation Mechanism  [Not invited]
    Kazuya Shimizu; Kunihiro Okano; Hideaki Maseda; Tomoaki Itayama; Yukio Kawauchi; Motoo Utsumi; Zhenya Zhang; Norio Sugiura
    13th World Lake Conference  2009/11  Keynote oral presentation
  • Removal characteristics of musty odor geosmin" by biofiltration using various carriers"  [Not invited]
    清水 和哉; Okei I; Xue Q; Shimizu K; Utsumi M; Sugiura N
    2nd China-Japan Graduated Student Forum  2009/09
  • Finding of microcystin-degrading bacterium and elucidation of its degradation mechanism  [Not invited]
    清水 和哉; Shimizu K; Okano K; Maseda H; Kawauchi Y; Utsumi M; Zhang Z; Sugiura N
    2nd China-Japan Graduated Student Forum  2009/09
  • Protozoa-assisted control of water bloom and removal of microcystins  [Not invited]
    清水 和哉; Li J; Shimizu K; Utsumi M; Sugiura N
    2nd China-Japan Graduated Student Forum  2009/09
  • Topology of microcystin degrading enzyme, MlrA  [Not invited]
    清水 和哉; Kobayashi H; Maseda H; Sugiura N; Shimizu K; Kobayashi M
    2nd China-Japan Graduated Student Forum  2009/09
  • microcystin分解菌におけるmicrocystin分解酵素遺伝子欠損株によるmicrocystin分解酵素の機能解析  [Not invited]
    清水 和哉; 清水和哉; 間世田英明; 岡野邦宏; 内海真生; 杉浦則夫
    第43回日本水環境学会  2009/03
  • microcystin分解菌におけるmicrocystin分解酵素遺伝子群の保存性  [Not invited]
    清水 和哉; 岡智彦; 岡野邦宏; 清水和哉; 内海真生; 杉浦則夫
    第43回日本水環境学会  2009/03
  • 特定遺伝子領域を用いた有毒藍藻類の新規分類手法の開発  [Not invited]
    清水 和哉; 伊藤聡史; 岡野邦宏; 清水和哉; 内海真生; 杉浦則夫
    第43回日本水環境学会  2009/03
  • 生物処理槽におけるmicrocystin分解菌の季節的動態解析  [Not invited]
    清水 和哉; 神保有亮; 澁谷幸代; 清水和哉; 内海真生; 杉浦則夫
    第43回日本水環境学会  2009/03
  • 生物処理槽におけるmicrocystin分解菌の季節的動態解析  [Not invited]
    神保有亮; 渋谷幸代; 清水和哉; 内海真生; 杉浦則夫
    第43回 日本水環境学会  2009/03  Oral presentation
  • microcystin分解菌におけるmicrocystin分解酵素遺伝子の保存性  [Not invited]
    岡智彦; 岡野邦宏; 清水和哉; 内海真生; 杉浦則夫
    第43回 日本水環境学会  2009/03  Oral presentation
  • microcystin分解菌におけるmicrocystin分解酵素遺伝子欠損株によるmicrocystin分解酵素の機能解析  [Not invited]
    清水和哉; 間世田英明; 岡野邦宏; 内海真生; 杉浦則夫
    第43回 日本水環境学会  2009/03  Oral presentation
  • 特定遺伝子領域を用いた有毒藍藻の比較解析  [Not invited]
    清水 和哉; 伊藤聡史; 岡野邦宏; 清水和哉; 内海真生; 杉浦則夫
    第45回日本水処理生物学会  2008/11
  • 分子生物学的手法によるmicrocystin分解酵素遺伝子群の検出  [Not invited]
    清水 和哉; 岡智彦; 岡野邦宏; 藤本尚志; 清水和哉; 内海真生; 杉浦則夫
    第45回日本水処理生物学会  2008/11
  • 藍藻由来生物毒microcystin分解酵素遺伝子の転写制御に関する研究  [Not invited]
    清水 和哉; 清水和哉; 岡野邦宏; 間世田英明; 雨宮崇; 内海真生; 杉浦則夫
    第45回日本水処理生物学会  2008/11
  • 分子生物学的手法によるmicrocystin分解酵素遺伝子群の検出  [Not invited]
    岡智彦; 岡野邦宏; 清水和哉; 内海真生; 杉浦則夫
    第45回 日本水処理生物学会  2008/11  Oral presentation
  • 特定遺伝子領域を用いた有毒藍藻の比較解析  [Not invited]
    伊藤聡; 岡野邦宏; 清水和哉; 内海真生; 杉浦則夫
    第45回 日本水処理生物学会  2008/11  Oral presentation
  • 藍藻由来生物毒microcystin分解酵素遺伝子の転写制御に関する研究  [Not invited]
    清水和哉; 岡野邦宏; 間世田英明; 雨宮崇; 内海真生; 杉浦則夫
    第45回 日本水処理生物学会  2008/11  Oral presentation
  • Present Situation of the Unpleasant Smell and Taste in Drinking water Source in Japan  [Not invited]
    清水 和哉; Nasuno A; Shimizu K; Utsumi M; Sugiura N
    1st Japan-China Graduated Student Forum  2008/10
  • Effect of Cyclic Microcystin and Its Degradation Products on Transcription of the Gene Encoding Micr  [Not invited]
    清水 和哉; Shimizu K; Okano K; Amemiya T; Maseda H; Utsumi M; Sugiura N
    1st Japan-China Graduated Student Forum  2008/10
  • Effect of Cyclic Microcystin and Its Degradation Products on Transcription of the Gene Encoding Microcystin Degrading Enzyme  [Not invited]
    Kazuya Shimizu; Kunihiro Okano; Takashi Amemiya; Hideaki Maseda; Motoo Utsumi; Norio Sugiura
    1st Japan-China Graduated Student Forum  2008/10  Poster presentation
  • Quantification and seasonal change of microcystin-degrading bacteria by real-time PCR  [Not invited]
    清水 和哉; Jimbo Y; Okano K; Shimizu K; Maseda H; Utsumi M; Sugiura N
    17th Japan/Korea Symposium on Water Environment  2008/09
  • 分子生物学的手法を導入した上水生物膜処理技術の開発  [Not invited]
    清水 和哉; 杉浦則夫; 澁谷幸代; 内海真生; 神保有亮; 清水和哉
    第11回日本水環境学会シンポジウム  2008/09
  • 分子生物学的手法を導入した上水生物膜処理技術の開発  [Not invited]
    杉浦則夫; 渋谷幸代; 内海真生; 神保有亮; 清水 和哉
    第11回 日本水環境学会シンポジウム  2008/09  Nominated symposium
  • Effect of Cyclic Microcystin and Its Degradation Products on Transcription of the Gene Encoding Microcystin Degrading Enzyme  [Not invited]
    清水 和哉; Shimizu K; Okano K; Maseda H; Amemiya T; Utsumi M; Sugiura N
    The 12th International Symposium on Microbial Ecology, Poster Presentation  2008/08
  • Effect of Cyclic Microcystin and Its Degradation Products on Transcription of the Gene Encoding Microcystin Degrading Enzyme  [Not invited]
    Kazuya Shimizu; Kunihiro Okano; Hideaki Maseda; Takashi Amemiya; Motoo Utsumi; Norio Sugiura
    The 12th International Symposium on Microbial Ecology  2008/08  Poster presentation
  • 藍藻毒microcystin分解酵素遺伝子に関する研究 III. 発現解析  [Not invited]
    清水 和哉; 雨宮崇; 清水和哉; 岡野邦宏; 間世田英明; 内海真生; 杉浦則夫
    第42回日本水環境学会年会  2008/03
  • ポリフェノールによる藍藻類の増殖阻害機構に関する研究  [Not invited]
    清水 和哉; 岡野邦宏; 清水和哉; 渡辺洋大; 杉浦則夫
    第42回日本水環境学会年会  2008/03
  • 藍藻由来生物毒microcystin分解酵素遺伝子に関する研究 I. 転写調節  [Not invited]
    清水 和哉; 清水和哉; 岡野邦宏; 雨宮崇; 間世田英明; 内海真生; 杉浦則夫
    第42回日本水環境学会年会  2008/03
  • ポリフェノールの自動酸化と藍藻類の増殖阻害機構に関する研究  [Not invited]
    清水 和哉; 岡野邦宏; 清水和哉; 杉浦則夫
    第44回日本水処理生物学会  2007/11
  • microcystin分解酵素遺伝子の転写に関する研究  [Not invited]
    清水 和哉; 雨宮崇; 清水和哉; 岡野邦宏; 間世田英明; 内海真生; 杉浦則夫
    第44回日本水処理生物学会  2007/11
  • ロックウール担体を用いた硝化細菌純粋培養株による硝化速度の効率化に関する研究  [Not invited]
    清水 和哉; 清水和哉; 岡野邦宏; 内海真生; 杉浦則夫
    第41回日本水環境学会  2007/03
  • Using rockwool as a carrier for the improvement of nitrification rate on the wastewater treatment of  [Not invited]
    清水 和哉; Shimizu K; Sugiura N; Maekawa T
    Opening Memorial Ceremony and Memorial Symposium of CIGR General Secretariat in Tsukuba  2006/03
  • 閉鎖系生活空間施設内における硝化速度の向上  [Not invited]
    清水 和哉; 清水和哉; 杉浦則夫; 前川孝昭
    第42回日本水処理生物学会  2005/11

MISC

Awards & Honors

  • 2020/11 筑波大学 若手教員奨励賞
  • 2013/08 International Conference on Interdisciplinary Research and Development in ASEAN Universities Best Paper Award
     Development of bio-fence for water purification of aquaculture ponds 
    受賞者: Tomoaki Itayama;Xueying Bi;Norio Iwami;Kazuya Shimizu;Chunyan Liu;Dong Xia;Redel Gutierrez;Niwooti Whangchai
  • 2011/11 Japanese Society of Water Treatment Bioogy Best Article Award
     Impact of nitrification inhibitor on nitrogen removal and related enzyme activities during soil infiltration treatment of domestic wastewater 
    受賞者: Lei Zhongfang;Huang Yingen;Shimizu Kazuya;Zhang Zhenya
  • 2009/03 Graduate School of Life and Environmental Sciences, University of Tsukuba Award
     
    受賞者: Kazuya Shimizu
  • 2008/10 1st Japan-China Graduated Student Forum Best Poster Presentation
     
    受賞者: 清水 和哉

Research Grants & Projects

  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2024/04 -2029/03 
    Author : 雷 中方; 原 田; 清水 和哉; 内海 真生; 水野谷 剛; 林 彬勒; 足立 泰久
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2022/04 -2025/03 
    Author : 清水 和哉; 岩見 徳雄; 間世田 英明; 岡野 邦宏; 板山 朋聡; 原 田; 張 振亜
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2020/10 -2025/03 
    Author : 辻村 真貴; 清水 和哉; 榊原 厚一
     
    クアラルンプール、クラン川流域において、これまでに採取した地下水、河川水、降水等の同位体、溶存成分分析結果を解析し、当該地域における水循環、とくに、地下水涵養源、地表水－地下水循環プロセスに関し、課題の整理を行った。あわせて、熱帯湿潤域における地下水涵養に関する既存研究のレビューを進めた。さらに、今後引き続き、新型コロナ感染症の影響で、現地フィールド調査が十分に行えない事態も考慮し、これまで得られたデータに加え、既存公開データ等を併用し、数値モデルを用いて対象地域の地下水涵養、水循環および人間活動と地下水－地表水循環との関係を考究する可能性を検討した。 その結果、年平均降水量の水文条件、人口密度等の人文条件を考慮すると、クアラルンプールは、従来、とくに同位体等を手法として用いた地下水、水文研究事例がきわめて少ない地域に該当することが示された。また、標高数100m以上における降水により、低地部の地下水が涵養されていることが示唆されたが、高標高部における降水の安定同位体に関するデータが不十分であることも示された。さらに、今後、WEAPモデルを本地域において適用することにより、当該地域の水循環プロセスがより明らかにされる可能性があることが示された。 2022年3月下旬から4月上旬にかけマレーシア・クアラルンプールを訪問し、マレーシア原子力機関、マレーシア国民大学等のカウンターパートと、今後の研究計画に関し意見交換を行うことができた。2020年2月以来実施できなかった直接打合せができたことにより、2022年度の現地調査が進むことが期待される。
  • 日本学術振興会：科学研究費助成事業
    Date (from‐to) : 2020/07 -2023/03 
    Author : 間世田 英明; 岩見 徳雄; 正井 博和; 清水 和哉; 梅津 一孝; 井原 一高; 板山 朋聡
  • 日本学術振興会：科学研究費助成事業 基盤研究(B)
    Date (from‐to) : 2020/04 -2023/03 
    Author : 井原 一高; 間世田 英明; 清水 和哉; 梅津 一孝
     
    検討項目1： 畜産廃水等の液相に残留する抗生物質の磁気分離除去法の確立のため，乳牛糞尿の固液分離後の液分を対象とし，抗生物質の磁気フィルタによる分離と無害化プロセスに接続するための磁気フィルタからの回収について検討した。電気化学凝集反応を用いた抗生物質への磁気シーディング法において，電流密度の影響は小さい一方で，投入電気量が除去率に影響することを明らかにした。磁気フィルタからの分離物回収手法の開発のため，マグネタイトを模擬物質として磁気力による分離と回収試験を実施した。対象物を分離後，脱磁操作が回収率向上に寄与することを示すとともに，磁気フィルタによる分離の諸条件が回収率に影響することを明らかにした。 検討項目2： メタン発酵による乳牛糞尿に残留する抗生物質耐性菌低減を目的として，嫌気性消化プロセスにおける酸生成過程とメタン生成過程における抗生物質耐性菌の消長について，乳牛糞尿を用いた2段階バッチ試験によって検討した。セファゾリンを添加した場合，酸生成過程においてVFA蓄積を招き，結果として耐性菌の消長に影響を与えた。メタン生成過程は，薬剤耐性菌低減の主たるフェーズであることが明らかになった。 検討項目3： 生物処理の前処理として、排水中の抗生物質を除去することが、いくつかの報告で示されている。しかし、微生物群集構造、排水処理性能、抗生物質耐性菌の出現などを引き起こさない最小限の抗生物質濃度が明らかでないため、前処理のガイドライン値は明らかにされていない。そこで本研究では、微生物群集構造および排水処理性能に影響を与えない最小限の抗生物質濃度を求めるため、排水処理性能および微生物群集の動的変化を解析した。得られた結果より，前処理時のLVXのガイドライン値として0.5 mg-LVX/L以下に設定することが提案できると考えられた。
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2019/04 -2022/03 
    Author : TAKANASHI Hirokazu
     
    The causative compound of earthy-musty odors in Japanese tap water is mainly geosmin. The causative microorganism is mainly Anabaena sp. in Japan. Recently, two types of Anabaena spp. were found in the same water body. One is capable of producing geosmin, while another is not. These strains were similar to each other in shape, size, and color, making microscopic examination difficult. Thus in this study, a discriminative analytical method using mass spectrometry was developed. The developed method successfully identified four geosmin-producing Anabaena spp. It can also discriminate between geosmin-producing and non-producing strains, and between Anabaena in the logarithmic growth phase and that in the dead phase. The limit of identification was 10% of the Anabaena.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2019/04 -2022/03 
    Author : UTSUMI MOTOO
     
    The removal of musty odor substances is a major expense in producing drinking water and causes economic loss of fishery products due to odor adsorption. This study was conducted to clarify the environmental factors under which actinomycetes, musty odor-producing microorganisms, produce musty odor substances and to develop musty odor control methods based on this knowledge. The results showed that the presence or absence of light did not affect the growth of actinomycetes. However, the wavelength and intensity of light affected their production of musty odor substances. In addition, it was found that the musty odorant was produced during the logarithmic growth phase under aerobic to microaerophilic conditions, and neither growth nor production of musty odorant under anaerobic conditions, indicating that the duration of anaerobic conditions affects the growth and production of musty odorant.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2018/04 -2022/03 
    Author : Lei Zhongfang
     
    Resource/energy recovery from wastewater is crucial for sustainable development of wastewater treatment plants (WWTPs). In this project, we achieved fruitful results on the following six aspects: (1) Rapid granulation and harvesting of natural microalgae and single cell microalgae; (2)Value-added biomaterials accumulation and production from wastewater, including phosphorus, alginate-like exopolymers (ALE), proteins, polysaccharides, and lipids; (3) Impacts of operation mode on the system stability and nutrients removal; (4) Granular system stability and remediation under various conditions; (5) Heavy metal (with Cr as example) recovery from wastewater and safe posttreatment of heavy metal-loaded granules; and (6) Deep insight into the biogranulation of bacteria/microalgae symbiosis and microalgae, highlighting the functions of specific microalgae species and extracellular polymeric substances (EPS).
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2018/06 -2021/03 
    Author : SHIMIZU Hidehisa
     
    In recent years, with the globalization of food, the possibility of ingestion of microcystin, a toxin derived from blue-green algae, through drinking water and freshwater seafood has been pointed out not only in developing countries but also in developed countries. In the present study, we examined the effects of chronic exposure to low concentrations of microcystin on organ function. The results showed that exposure to low concentrations of microcystin induced functional disorders in the liver and colon. Analysis using cultured hepatocytes and intestinal epithelial cells suggested the existence of unconventional pathways of microcystin action. Based on the results of present study, we expect to further elucidate the mechanism of action and search for biomarkers for chronic microcystin poisoning in the future.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2018/04 -2021/03 
    Author : SHIMIZU Kazuya
     
    In this study, we isolated predators of microcystin-producing cyanobacteria, closely related predators regardless of the region, and isolated novel predators. In addition, we developed an aquatic remediation technology using these predators, their symbiotic microcystin-degrading bacteria, and algal-bacterial granular sludge. We found that predators that are not symbiotic with microcystin-degrading bacteria are affected by microcystin toxicity, and that microcystin acts as a defense mechanism for the producing cyanobacteria. Furthermore, our results suggest an increase in microcystin predation pressure by symbiotic bacteria. This study has created an analytical tool for constructing aquatic environmental remediation technologies that can be used in the field, focusing on symbiotic bacteria as predators, and aquatic purification technologies that can be used in the field.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
    Date (from‐to) : 2016/04 -2019/03 
    Author : IHARA IKKO
     
    In order to prevent the diffusion of antibiotic-resistant bacteria to the environment, we developed a magnetic separation method as an on-site treatment technology of antibiotics contained in livestock wastewater, and examined the relationship between resistance gene mutations and antibiotic concentration to avoid the appearance of resistant bacteria, and evaluated the appearance risk of resistant bacteria. We developed a magnetic separator with permanent magnets. It was found that the removal rate of antibiotics was drastically improved by controlling pH in the magnet seeding. The risk of appearance of resistant bacteria caused by antibiotics used in the livestock industry was evaluated. First of all, microbial community structure changes by their exposures were investigated in a model system using an antibiotic. Though the environmental risk by the antibiotic was transient, it was clarified that the risk could be reduced by appropriately reducing the antibiotics.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
    Date (from‐to) : 2015/04 -2018/03 
    Author : SHIMIZU Kazuya; Niwooti Whangchai; IWAMOTO Iwamoto; Zuriati Zakaria
     
    In this study, we completed the whole genome sequence of microcystin-degrading bacteria, and then made and used various molecular breeding strains to identify microcystin degradation pathway and intracellular localization of microcystin-degrading enzymes. We got the results that MlrA degrade most microcystin analogues. In addition, we have constructed an aquatic environment restoration technology by useful bacteria, protozoa, and micro-animal that can be utilized at the site which is occurred cyanobacterial bloom. This research has shown the possibility of innovating water treatment technology utilizing molecular breeding strains and also provides the aquatic environment restoration and water treatment technology that can be used on the actual site.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research
    Date (from‐to) : 2015/04 -2017/03 
    Author : Shimizu Hidehisa; SUGIURA NORIO; MIYAZAKI HITOSHI; ISHIZUKA SATOSHI; FUKITANI SATORU
     
    Eutrophication of lake and reservoir accelerates development of Microcystis which produces a toxin, called microcystin. The present study shows that in a cultured hepatocyte cell line, HepG2 cells, AMPK was time- and dose-dependently activated by microcystin-LR (MC-LR) stimulation. When AMPK was not activated in MC-LR-treated HepG2 cells, cell death was induced. However, when MC-LR activated AMPK, cell death was inhibited. In a cultured intestinal cell line, Caco-2 cells, MC-LR leaded to cell proliferation through p38 and JNK activation although ERK activation was not participate. Furthermore, the expression levels of oncogene was upregulated in MC-LR-treated Caco-2 cells. In rat analysis, food intake, body weight, and weight of liver, kidney, and adipose tissue were not difference between control and MC-LR-administrated rats. We will continue to check for serum parameter (ALT/AST, TG, and TC etc), liver TG and TC, and gut microbiota, etc.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
    Date (from‐to) : 2014/04 -2017/03 
    Author : SUGIURA Norio; ZAKARIA Zuriati; IWAMOTO Kouji
     
    We conducted a survey and social scientific analysis of Batu dam river from Batu dam, titiwangusa lake which is recreation lake, and two drinking water reservoirs, semenyih dam and Batu dam for Kuala Lumper city, Malaysia. The water reservoir dam lakes were good water quality focusing on nitrogen, phosphorus, and Chl.a concentration. However, microscope observation and eco-genomics analysis revealed microcystin-producing cyanobacteria, Microcystis sp. in lake water bodies. Therefore, it is important to maintain good water source quality, and social scientific analysis revealed that new Japanese technology can be implemented socially by constructing a social system of waste recycling for watershed management that contributes to maintain water source quality.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
    Date (from‐to) : 2013/04 -2017/03 
    Author : ITAYAMA Tomoaki; Niwooti Whangchai; Ruenkeaw Praphrute; Korntip Kannika; Rattapoom Prommana; Redel Gutierrez; Louis Lebel; Khomsan Ruangrit
     
    We surveyed aquaculture ponds at different altitudes in order to evaluate the effect of global warming for proliferation of toxic cyanobacteria. Water temperature, nutrients, chlorophyll-a (phytoplankton surrogate), cyano-toxin microcystin were measured. Phycocyanin-ITS gene (total cyanobacteria surrogate), microcystin synthesis gene mcyB (toxic cyanobacteria with producing microcystin surrogate) were quantified by real-time PCR. We analyzed the obtained data by statistical model. The results clearly showed the following facts. The increment of water temperature and the increase of both N and P loading enhanced the growth of phytoplankton. Increment of water temperature and increase of nutrients N loading enhanced the growth of the total cyanobacteria and the toxic cyanobacteria. However, we found new fact that the standing crops of cyano-toxin microcystin was suppressed by the increment of water temperature, though the increase of nutrients N loading added to microcystin.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
    Date (from‐to) : 2013/04 -2016/03 
    Author : SUGIURA Norio; UTSUMI Motoo; MASEDA Hideaki; SHIMIZU Kazuya
     
    In recent years, the occurrence of musty odor originated from microorganisms in freshwater environments has been reported in all over the world. In this study, we studied that what kind of environmental factors influence to musty odor production by actinomycetes and how to change musty odor production when the environmental factor has changed. As a result, the difference of wavelength and intensity of visible light, and dissolved oxygen concentration and its variation affected musty odor production by actinomycetes. Also, we found that the musty odor-degrading microbes existed in the biofilm of a practical biological water treatment plant throughout the year, and the musty odor degradation rate changed throughout a year, indicating that musty odor degradation rates were greatly affected by water temperature. Moreover, although the musty odor degradation rate was inhibited at lower temperatures, it can be recovered at appropriate water temperature.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
    Date (from‐to) : 2013/04 -2016/03 
    Author : ZHANG Zhenya; YANG Yingnan; LEI Zhongfang; SHIMIZU Kazuya
     
    This study aims at (1) recovery nitrogen resources, (2) phosphorus recovery from solid organic waste, and (3) establish the recovery process. Also, 15 papers have been published. The TP concentration in primary sludge, secondary sludge and digested sludge was investigated respectively, and the conditions for recovery P was established. Pretreatment of manure for breakdown of the complex fiber structures and disintegration of solid organic-N to produce ammonia is beneficial for enhancing manure digestibility and the downstream N recovery. Short-term dry AD and hydrothermal treatment (HTT) were applied respectively for degradation of the solid manure. This section explored the feasibility of comprehensive utilization of C, N and P resources in swine manure (SM) through short-term dry AD (anaerobic digestion) followed by dry ammonia stripping. Results from this work reflected an overall N recovery efficiency of 57% from CM and increase in methane yield by 24% (compared to raw CM).
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
    Date (from‐to) : 2012/04 -2016/03 
    Author : IWAMI, norio; ITAYAMA tomoaki; SHIMIZU kazuya; SHIOYA kazune
     
    In this study, we examined an appearing micro-fauna and an impact to predation pressure by artificial disassembling of Microcystis cell colony. Furthermore, we considered a process of reducing Microcystis in predation pressure of the identified micro-fauna. As a result, protozoan flagellate Monas sp. as a predator of singly disperse cells of Microcystis was estimated by enrichment culture system. In addition, it was confirmed by the combined culture system that the predation pressure of isolated Monas sp. is increased by artificially disassembling colony formed Microcystis to single cells. It was suggested that suppression of Microcystis on small pond was achieved by results of the model experiment which the small scale pond combined with the predation effect of Monas sp. and the system of artificially disassembling Micorcystis cell colony.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)
    Date (from‐to) : 2012/04 -2016/03 
    Author : Kishida Naohiro; SHIMIZU Kazuya
     
    The specificity of quantitative polymerase chain reaction (qPCR) assays targeting geosmin synthase gene was evaluated using cyanobacterial strains isolated form domestic water sources. Then selected high specific qPCR assay was applied to analyze water samples obtained from one water reservoir where geosmin producing cyanobacteria often occurs. The geosmin synthase gene was successfully detected even when geosmin concentration was low. In addition, the number of geosmin synthase genes analyzed by the qPCR was well correlated with geosmin concentration in the water reservoir. These results demonstrated that the qPCR assay can be used as an early, easy and quantitative detection method of geosmin-producing cyanobacteria in drinking water sources.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)
    Date (from‐to) : 2012/04 -2015/03 
    Author : SHIMIZU Kazuya
     
    In the present study, environmental factor for microcystin degradative activity and enzymatic pathway of microcystin degradation in microcystin degrading bacterium were revealed. In aquatic environment, the relationship between microcystin degradation and microcystinase gene, mlrA is one to one. This relationship is very interesting because most of the cases are not one to one relation. The mlrA gene is unique. The rapid microcystin degradation in aquatic environment and drinking water treatment system is supposed that microcystin sequential degradation is occurred by interaction between microcystin degrading bacteria and microcystin degradation related microorganisms in microbial community.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
    Date (from‐to) : 2011/04 -2014/03 
    Author : KASHIWADA Shosaku; TATSUTA Haruki; NAGASAKA Seiji; HIROTSU Naoki; SAKAMOTO Masaki; SHIMIZU Kazuya; TAKEI Hiroyuki; KATSUMATA Masakazu
     
    Minimum bactericidal concentration of silver nanocolloids (SNCs) to active sludge was 5 mg/L. Toxicities of SNCs to cyanobacteria and micro algae were comparatively high, in addition, nano-size dependent toxicity was observed. In case of SNCs exposure to aquatic plants, there was inhibition of roots growth because released Ag+ inhibited glutathione reductase and following increase of H2O2 in roots reduced catalase activity. There is no significant difference between SNCs and AgNO3 in acute mobility inhibition test of invertebrates. Main toxic factor in reproduction test of invertebrates was Ag+. In fish, it was observed that shortened body length, reduction of heart beating, ischemia and kyphosis were induced by SNCs exposure. SOD, CAT and GSH were significantly reduced, and apoptosis inhibition was observed. Moreover, intrinsic growth rate of fish was significantly decreased by SNCs exposure.
  • 日本学術振興会：科学研究費助成事業
    Date (from‐to) : 2009 -2011 
    Author : 板山 朋聡; 岩見 徳雄; 杉浦 則夫; 清水 和也
     
    タイのメジョー大実験養魚池と商用養魚池(ナマズ養魚池とテラピア養魚池)で、microcystin (MC)濃度測定、16SrDNAの全藍藻とMicyorocystis sp.、MC合成遺伝子mcyaDとmcyE(Microcystis特異的)の定量PCRを実施した結果、全項目でテラピア池よりナマズ池が高かった。MC濃度とmcyDコピー数の間にナマズ池で正の相関があったが、テラピア池は明確な相関がなかった。使用したmcyD-primerはMicrocystis以外にPlanktothrixやAnabaenaのmcyDを増幅すること、種毎にMC産生能が異なることから、藍藻種の遷移がMC濃度/mcyD比に影響することもこの原因の一つと推察された。また両池ともにmcy保有藍藻中のMicrocystisは10%以下であり、多くのmcyがPlanktotrixやAnabaena由来であることも示唆された。養魚池から単離のMicrocystisもmcyD非保有株であった。MC-LR/-RR/-YR比もナマズ池とテラピア池では異なり、時間的に変化したことも藍藻種構成の遷移を裏付けた。一方、MC分解遺伝子mlrAコピー数とMC濃度には正の相関があった。霞ヶ浦土浦港に比べ実験養魚池のmlrA/MC濃度比は高く、このことから高いMC分解活性が実験養魚池のMC濃度を低下させ、MC濃度/mcyD比が土浦港より実験養魚池で1/10以下となった原因の一つと推察された。さらに、有毒藍藻(mcyD)発生頻度は全リン濃度に対するロジスティック回帰推定が有効であった。また1μg/L以下のMC-RR,YRとカビ臭物質Geosminと2MIBが養魚から検出され養魚池浄化が必要である為、木炭担体フェンス型生物ろ過装置により藍藻とMCが除去可能なことを示し、藍藻増殖-浄化モデルによって有効な養魚池浄化法を検討した。
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2009 -2011 
    Author : ITAYAMA Tomoki; IWAMI Norio; SUGIURA Norio; SIMIZU Kazuya; SANO Tomoharu; PARK Hodong; WHANGCHAI Niwoot; PRAPHRUTE Ruenkeaw; KANNIKA Korntip; PROMMANA Rattapoom; GUTIERREZ Redel; PEERAPORNPISA Yuwadee
     
    We found toxic cyanobacteria such as Microcystis sp. in almost investigated fish ponds(including the experimental fish ponds of Maejo University) in North Thailand. Especially, the amount of toxin producing cyanobacteria in the catfish pond was much than that in the tilapia ponds. However we also found the high ratio of non-toxin-producing strains of Microcystis sp. in such fish ponds by a cyanotoxin microcystin analysis and qPCR for the microcystin synthetase mcy genes. A few amount of microcystin was detected from several fishes in the experimental ponds in Maejo University. On the other hand, musty odors substances produced by cyanobacteria were detected in many fishes and in many ponds. Therefore, because we thought that it is important to clarify such fish ponds using a bio-fence type biological filtration method, we conducted a preliminary experiment and mathematical analysis of purification process of the ponds. As a result, we presented that the bio-fence method should be developed as an adequate eco-engineering for safe fish production in aquaculture.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
    Date (from‐to) : 2009 -2011 
    Author : SUGIURA Norio; UTSUMI Motoo; MASEDA Hideaki; OKANO Kunihiro; SHIMIZU Kazuya
     
    In the present study, we revealed enzymatic pathway of microbial microcystin degradation in microcystin-degrading bacterium. The abundance of mlrA gene in aquatic environment strongly related to microcystin concentration, thus microcystin-degrading bacteria play a key role in microcystin degradation. Moreover, we confirmed that Adda induce expression of mlrA and mlrB, and cyclic structure of microcystin relate to expression of mlrC. Finally, we have constructed new water treatment apparatus using electrochemical degradation without electrolyte. The apparatus inhibit toxic cyanobacteria growth, but do not inhibit other organism, such as animal plankton and fish.

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