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Akihiko Ichiishi

Faculty
Department of Life Sciences
Course of Life Sciences
PositionProfessor
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Birthday
Last Updated :2019/10/04

Researcher Profile and Settings

Association Memberships

  • THE GENETICS SOCIETY OF JAPAN
  • THE MOLECULAR BIOLOGY SOCIETY OF JAPAN
  • JAPAN SOCIETY FOR BIOSICENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY

Research Activities

Research Areas

  • Basic biology, Genetics/Chromosome dynamics, DNA repair

Research Interests

    Nucleotide excision repair, photorepair, DNA repair, mutagenesis, Neurospora crassa

Published Papers

  • Deletion and expression analysis of beta-(1,3)-glucanosyltransferase genes in Neurospora crassa., Kamei M, Yamashita K, Takahashi M, Fukumori F, Ichiishi A, Fujimura M, Fungal genetics and biology : FG & B, 52, 65 - 72, 03 , Refereed
  • An AP-1-like transcription factor, NAP-1, regulates expression of the glutathione S-transferase and NADH:flavin oxidoreductase genes in Neurospora crassa., Takahashi M, Yamashita K, Shiozawa A, Ichiishi A, Fukumori F, Fujimura M, Bioscience, biotechnology, and biochemistry, 74, 746 - 752, Refereed
  • Genotyping of benzimidazole-resistant and dicarboximide-resistant mutations in Botrytis cinerea using real-time polymerase chain reaction assays., Banno S, Fukumori F, Ichiishi A, Okada K, Uekusa H, Kimura M, Fujimura M, Phytopathology, 98, 397 - 404, 04 , Refereed
  • Genetic analysis of the Neurospora crassa RAD14 homolog mus-43 and the RAD10 homolog mus-44 reveals that they belong to the mus-38 pathway of two nucleotide excision repair systems., Sato M, Niki T, Tokou T, Suzuki K, Fujimura M, Ichiishi A, Genes & genetic systems, 83, 1 - 11, 02 , Refereed
  • OS-2 mitogen activated protein kinase regulates the clock-controlled gene ccg-1 in Neurospora crassa., Watanabe S, Yamashita K, Ochiai N, Fukumori F, Ichiishi A, Kimura M, Fujimura M, Bioscience, biotechnology, and biochemistry, 71, 2856 - 2859, 11 , Refereed
  • Involvement of OS-2 MAP kinase in regulation of the large-subunit catalases CAT-1 and CAT-3 in Neurospora crassa., Yamashita K, Shiozawa A, Banno S, Fukumori F, Ichiishi A, Kimura M, Fujimura M, Genes & genetic systems, 82, 301 - 310, 08 , Refereed
  • Identification of OS-2 MAP kinase-dependent genes induced in response to osmotic stress, antifungal agent fludioxonil, and heat shock in Neurospora crassa., Noguchi R, Banno S, Ichikawa R, Fukumori F, Ichiishi A, Kimura M, Yamaguchi I, Fujimura M, Fungal genetics and biology : FG & B, 44, 208 - 218, 03 , Refereed
  • An Os-1 family histidine kinase from a filamentous fungus confers fungicide-sensitivity to yeast., Motoyama T, Ohira T, Kadokura K, Ichiishi A, Fujimura M, Yamaguchi I, Kudo T, Current genetics, 47, 298 - 306, 05 , Refereed
  • A two-component histidine kinase of the rice blast fungus is involved in osmotic stress response and fungicide action., Motoyama T, Kadokura K, Ohira T, Ichiishi A, Fujimura M, Yamaguchi I, Kudo T, Fungal genetics and biology : FG & B, 42, 200 - 212, 03 , Refereed
  • Putative homologs of SSK22 MAPKK kinase and PBS2 MAPK kinase of Saccharomyces cerevisiae encoded by os-4 and os-5 genes for osmotic sensitivity and fungicide resistance in Neurospora crassa., Fujimura M, Ochiai N, Oshima M, Motoyama T, Ichiishi A, Usami R, Horikoshi K, Yamaguchi I, Bioscience, biotechnology, and biochemistry, 67, 186 - 191, 01 , Refereed
  • Effects of iprodione and fludioxonil on glycerol synthesis and hyphal development in Candida albicans., Ochiai N, Fujimura M, Oshima M, Motoyama T, Ichiishi A, Yamada-Okabe H, Yamaguchi I, Bioscience, biotechnology, and biochemistry, 66, 2209 - 2215, 10 , Refereed
  • A Point Mutation in the Two-Component Histidine Kinase BcOS-1 Gene Confers Dicarboximide Resistance in Field Isolates of Botrytis cinerea., Oshima M, Fujimura M, Banno S, Hashimoto C, Motoyama T, Ichiishi A, Yamaguchi I, Phytopathology, 92, 75 - 80, 01 , Refereed

Misc

  • アカパンカビのβ‐1,3‐glucanosyltransferase遺伝子群の細胞壁損傷処理による発現解析, 亀井誠之, 高橋正和, 一石昭彦, 藤村真, 日本農芸化学会関東支部受賞記念講演およびシンポジウム講演要旨集, 2011,   2011 10 15
  • Congratulating Prof. Makoto FUJIMURA on Winning the Honorable Achievements Award (Study) from Pesticide Science Society of Japan,   2011
  • アカパンカビのDNA修復変異株におけるフルジオキソニルおよびイプロジオン耐性菌の出現頻度, 穴澤初夫, 高橋司, 田中志門, 矢部秀和, 藤村真, 一石昭彦, 日本農薬学会大会講演要旨集, 35th,   2010 04 21
  • アカパンカビのフルジオキソニル耐性菌の分生子生存活性の低下とその機構, 山下和宏, 高橋正和, 亀井誠之, 一石昭彦, 藤村真, 日本農薬学会大会講演要旨集, 35th,   2010 04 21
  • 細胞壁合成阻害剤micafunginがNeurospora crassaの3種類のMAPキナーゼに与える影響, 亀井誠之, 山下和宏, 高橋正和, 一石昭彦, 藤村真, 日本農薬学会大会講演要旨集, 35th,   2010 04 21
  • アカパンカビの転写調節因子ATF‐1の局在解析およびリン酸化解析, 山下和宏, 高橋正和, 福森文康, 一石昭彦, 藤村真, 日本農芸化学会大会講演要旨集, 2009,   2009 03 05
  • アカパンカビのAP1様転写因子NAP‐1の制御遺伝子の同定と細胞内局在性解析, 高橋正和, 山下和弘, 石川智子, 福森文康, 一石昭彦, 藤村真, 日本農芸化学会大会講演要旨集, 2009,   2009 03 05
  • 過酸化水素耐性菌由来カタラーゼの精製とバイオセンサへの利用, 松浦宇宏, 酒井美鈴, 戸部文絵, 一石昭彦, 岡崎渉, 大熊廣一, 日本農芸化学会大会講演要旨集, 2009,   2009 03 05
  • アカパンカビのDNA修復変異株におけるフルジオキソニル耐性菌の出現頻度, 穴澤初夫, 藤村真, 一石昭彦, 日本農薬学会大会講演要旨集, 34th,   2009 02 27
  • Message from Molecular Diagnosis Team of Plant Function Research Center, Tokyo University, Plant protection, 63, (2) 122 - 125,   2009 02
  • アカパンカビのストレス応答MAPKカスケードは概日リズムの出力経路の一端を担う, 山下和宏, 高橋正和, 渡邊節子, 福森文康, 一石昭彦, 藤村真, 日本遺伝学会大会プログラム・予稿集, 80th,   2008 08 08
  • アカパンカビのAP1様転写因子NAP‐1により制御される遺伝子の解析, 高橋正和, 山下和宏, 塩澤あずさ, 福森文康, 一石昭彦, 藤村真, 日本遺伝学会大会プログラム・予稿集, 80th,   2008 08 08
  • カタラーゼ産生微生物とその酵素, 岡崎渉, 西村達也, 一石昭彦, 大熊廣一, 日本農芸化学会大会講演要旨集, 2008,   2008 03 05
  • アカパンカビにおける出芽酵母RAD4,RAD23ホモログ遺伝子の解析, 星野正幸, 祖谷小百合, 一石昭彦, 日本分子生物学会年会講演要旨集, 28th,   2005 11 25
  • Pyrrolnitrin Interferes with Osmotic Signal Transduction in Neurospora crassa, OKADA Akiyoshi, BANNO Shinpei, ICHIISHI Akihiko, KIMURA Makoto, YAMAGUCHI Isamu, FUJIMURA Makoto, Journal of pesticide science, 30, (4) 378 - 383,   2005 11 20 , Pyrrolnitrin, produced by several bacteria that are used in biological control, has an inhibitory effect on the electron transport system of respiration in Neurospora crassa. We have previously described that fludioxonil, a derivative of phenylpyrroles, affects osmotic signal transduction. Both pyrrolnitrin and fludioxonil were highly active against Botrytis cinerea, Fusarium oxysporum, Rhizoctonia solani, and N. crassa. However, a high concentration of pyrrolnitrin (more than 10μg/ml) inhibited the growth of fludioxonil-insensitive fungi such as Pythium ultimum, Phytophthora capsici, and Saccharomyces cerevisiae. In order to clarify the difference in the antifungal mechanisms between pyrrolnitrin and fludioxonil, we observed cross-resistance in mutants of the osmotic signal transduction pathway, namely, os-1 (histidine kinase), os-4 (MAPKK kinase), os-5 (MAPK kinase), and os-2 (MAP kinase) of N. crassa. All os mutants that were resistant to fludioxonil showed cross-resistance to pyrrolnitrin without exception. The levels of resistance to pyrrolnitrin correlated well with those to fludioxonil in the 10 os-1 mutant alleles with single amino acid substitutions. However, at a concentration of 6.1μg/ml, pyrrolnitrin inhibited the growth of all strains including the os mutants insensitive to fludioxonil even at 25μg/ml. When the conidia of the wild-type strain were grown on a medium containing either fungicide at a concentration of 0.1μg/ml, both fungicides induced the swelling and rupture of conidia without germ-tube formation. At a concentration of 25μg/ml, pyrrolnitrin inhibited conidia germination without any morphological change in the fludioxonil-insensitive os-5 mutant. Both fungicides at a concentration of 1μg/ml stimulated glycerol synthesis in the wild-type strain, but the glycerol content was reduced to a considerable extent on treatment with 25μg/ml pyrrolnitrin. These results suggest that a primary antifungal mechanism of pyrrolnitrin against N. crassa is interference with the osmotic signal transduction pathway rather than inhibition of respiration.
  • イネいもち病のOs‐1ファミリーヒスチジンキナーゼHik1の機能ドメインの解析, 本山高幸, 大平寛大, 一石昭彦, 工藤俊章, 日本農芸化学会大会講演要旨集, 2005,   2005 03 05
  • シロイヌナズナによるペンタクロロフェノール(PCP)のファイトレメディエーション, 門倉香, 本山高幸, 大平寛大, 中村卓, 一石昭彦, 工藤俊章, 山口勇, 日本農芸化学会大会講演要旨集, 2003,   2003 03 05
  • キャベツ病原糸状菌Fusarium oxysporumのMAPキナーゼ遺伝子の機能解析, 大平寛大, 門倉香, 本山高幸, 一石昭彦, 有江力, 日本農芸化学会大会講演要旨集, 2002,   2002 03 05
  • 形質転換シロイヌナズナによるペンタクロロフェノール(PCP)のファイトレメディエーション, 門倉香, 大平寛大, 本山高幸, 一石昭彦, 山口勇, 日本農薬学会大会講演要旨集, 27th,   2002 02 22
  • Phytoremediation of pentachlorophenol (PCP) by transgenic Arabidopsis thaliana, Kadokura K., Ohira Y., Motoyama T., Ichiishi A., Yamaguchi I., 27,   2002 02 22
  • Sensitivity to Phenylpyrrole Fungicides and Abnormal Glycerol Accumulation in Os and Cut Mutant Strains of Neurospora crassa, FUJIMURA Makoto, OCHIAI Noriyuki, ICHIISHI Akihiko, USAMI Ron, HORIKOSHI Koki, YAMAGUCHI Isamu, Journal of pesticide science, 25, (1) 31 - 36,   2000 02 20 , Neurospora osmotic sensitive strains with os-1, os-2, os-4 and os-5 mutations showed cross-resistance to dicarboximides, aromatic hydrocarbons and fludioxonil. The os-2, os-4, and os-5 mutant strains were highly resistant to them, while the os-1 mutant strain was moderately resistant and its growth was inhibited by fludioxonil (LD_<50> : 0.087μg/ml) and iprodione (LD_<50> : 14μg/ml). Another osmotic sensitive mutation cut did not give resistance to these fungicides. The conidia of wild-type and cut strains swelled and burst without germination on the medium containing fludioxonil, and bursting of conidia was rescued by high osmotic pressure. Stimulation of glycerol biosynthesis by fludioxonil and iprodione observed in the wild-type strain was not induced in os-2, os-4 and os-5 mutant strains. The moderately resistant os-1 mutant strain accumulated substantial quantities of glycerol by 10μg/ml of fludioxonil, but produced less glycerol by 10μg/ml of iprodione. Thus it was assumed that fungal toxicity of these fungicides might be due to abnormal glycerol accumulation. However, both fludioxonil and iprodione did not induce glycerol biosynthesis in the fungicide-sensitive cut mutant strain. In response to osmotic stress, os mutant strains accumulated less amount of glycerol than the wild-type strain, whereas cut mutant strain did not produce glycerol in the medium containing 4% NaCl. Despite its lack of ability of glycerol biosynthesis in cut mutant strain, wild-type and cut strains were not distinguishable in their modes of fungal toxicity of fludioxonil. These data suggest that dicarboximides, aromatic hydrocarbons and fludioxonil interfere with the osmotic signal transduction pathway resulting in stimulation of glycerol biosynthesis, but abnormal glycerol accumulation is not essential for their fungal toxicity.
  • Cloning, nucleotide sequence, and expression of tef-1, the gene encoding translation elongation factor 1α(EF-1α)of Neurospora crassa, ICHI-ISHI Akihiko, INOUE Hirokazu, The Japanese journal of genetics, 70, (2) 273 - 287,   1995 04 25
  • Analysis of Neurospora crassa tef mutants., ICHIISHI AKIHIKO, INOUE KOICHI, 日本遺伝学会大会プログラム・予稿集, 66th,   1994 10
  • 2. Effects of Abscisic Acid and High Osmolarity of Culture Medium on the Development of Freezing Tolerance of Suspension-Cultured Cells of Wheat(Triticum monococcum L.)(Papers presented at the 39th Annual Meeting), SAITO Takayuki, ICHI-ISHI Akihiko, SUGAWARA Yasutake, 39,   1993 10 20 , Suspension-cultured cells of wheat (Triticum monococcum L.) were cultured in media containing abscisic acid (ABA) and high concentrations (0.44 or 0.88M) of mannitol or sucrose and changes in their freezing tolerance were investigated. In a medium with ABA, freezing tolerance of the cells increased rapidly and achieved a maximal level after 7-10 days of culture. Optimal concentration of ABA for increasing the freezing tolerance was 10 to 20 mg/l. Survival rate of the cells which were cultured in a medium containing 10mg/l ABA for 7 days was very high even at the freezing temperature of -50℃, whereas that of control cells was very low (<10%) at -15℃. Increase in the freezing tolerance was also observed in a mannitol or sucrose medium but the effect of these substances was less than that of ABA. The osmolarity of cell extracts from the cells increased with these treatments. However, there was no correlation between the degree of freezing tolerance and the osmolarity of cell extract. Protein analysis using SDS-PAGE showed that distinct changes in protein profile were induced by ABA in the medium but not induced by high concentrations of sucose or mannitol. These results suggest that different mechanisms are involved in the development of freezing tolerance of these cells for the two treatments.
  • Effects of Abscisic Acid and High Osmolarity of Culture Medium on the Development of Freezing Tolerance of Suspension-Cultured Cells of Wheat (Triticum monococcum L.)., SAITO TAKAYUKI, ICHIISHI AKIHIKO, SUGAWARA YASUTAKE, 凍結及び乾燥研究会会誌, 39,   1993 10
  • アカパンカビのEF‐1遺伝子のクローニングとその塩基配列, 一石昭彦, 井上弘一, 日本遺伝学会大会プログラム・予稿集, 65th,   1993 09
  • Induction of stress tolerance and cellular changes in cultured cells of Triticum monococcum by abscisic acid., SAITO TAKAYUKI, ICHIISHI AKIHIKO, ISHII CHIZU, SUGAWARA YASUTAKE, 植物組織培養学会大会,シンポジウム講演要旨集, 13th,   1993 07
  • コムギ培養細胞のABAおよび高浸透圧培地による耐凍性の増加, 斉藤孝之, 一石昭彦, 菅原康剛, 凍結及び乾燥研究会講演要旨集, 39th,   1993
  • Characterization of somatic hybrids between Triticum monococcum and Aegilops squarrosa., 一石昭彦, 石井千津, 荻原保成, Jpn J Breed, 41,   1991
  • Analysis of two regeneration systems in graminae., ISHII CHIZU, ISHIYAMA SEIJI, ICHIISHI AKIHIKO, Jpn J Breed, 38,   1988 10
  • Analysis of two regeneration systems in Graminae, Ishii C., Ishiyama S., Ichiishi A., Japanese Journal of Breeding, 38, (2) 182 - 183,   1988

Conference Activities & Talks

  • Analysis of photoreactivation in the NER-related gene defective strain of Neurospora crassa, Kotaro Tsukada, Akihiko Ichiishi,   2018 03 16
  • Expression analysis of phr gene from Neurospora crassa, Ryo Ishibashi, Akihiko Ichiishi,   2017 11 16