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Noriyuki Doukyu

Faculty
Department of Applied Biosciences
Bio-Nano Electronics Research Center
Course of Life Sciences
PositionProfessor
Mail
HomepageURL
Birthday
Last Updated :2020/07/09

Researcher Profile and Settings

Education

  •  - 1998 , Tokyo Institute of Technology, Bioscience and Biotechnology
  •  - 1998 , Tokyo Institute of Technology, Graduate School, Division of Life Science and Engineering
  •  - 1993 , Tokyo Institute of Technology, School of Engineering
  •  - 1993 , Tokyo Institute of Technology, Faculty of Engineering

Academic & Professional Experience

  •   1998  - 2003 , Tokyo Institute of Technology, Bioscience and Biotechnology

Research Activities

Research Areas

  • Life sciences, Applied microbiology
  • Life sciences, Applied biochemistry
  • Life sciences, Applied microbiology
  • Manufacturing technology (mechanical, electrical/electronic, chemical engineering), Applied biofunctional and bioprocess engineering

Research Interests

    Chromobacterium, AcrAB, marA, rob, soxS, Bioengineering, Applied Microbiology

Published Papers

  • Cholesterol oxidase with high catalytic activity from Pseudomonas aeruginosa: Screening, molecular genetic analysis, expression and characterization, Noriyuki Doukyu, Shyou Nihei, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 120, (1) 24 - 30, 07 , Refereed
  • Improvement of organic solvent tolerance by disruption of the ion gene in Escherichia coli, Rei Watanabe, Noriyuki Doukyu, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 118, (2) 139 - 144, 08 , Refereed
  • Hyper-activation of foldase-dependent lipase with lipase-specific foldase, Hiroyasu Ogino, Sosuke Inoue, Masahiro Yasuda, Noriyuki Doukyu, JOURNAL OF BIOTECHNOLOGY, JOURNAL OF BIOTECHNOLOGY, 166, (1-2) 20 - 24, 06 , Refereed
  • Halarchaeum salinum sp nov., a moderately acidophilic haloarchaeon isolated from commercial sea salt, Yuto Yamauchi, Hiroaki Minegishi, Akinobu Echigo, Yasuhiro Shimane, Hirokazu Shimoshige, Masahiro Kamekura, Takashi Itoh, Noriyuki Doukyu, Akira Inoue, Ron Usami, INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 63, 1138 - 1142, 03 , Refereed
  • Halarchaeum rubridurum sp. nov., a moderately acidophilic haloarchaeon isolated from commercial sea salt samples., Yamauchi Y, Minegishi H, Echigo A, Shimane Y, Kamekura M, Itoh T, Ohkuma M, Doukyu N, Inoue A, Usami R, Int J Syst Evol Microbiol., Int J Syst Evol Microbiol., 63, (9) 3143 - 3147, Refereed
  • Improvement in organic solvent tolerance by double disruptions of proV and marR genes in Escherichia coli, N. Doukyu, K. Ishikawa, R. Watanabe, H. Ogino, JOURNAL OF APPLIED MICROBIOLOGY, JOURNAL OF APPLIED MICROBIOLOGY, 112, (3) 464 - 474, 03 , Refereed
  • Contributions of mutations in acrR and marR genes to organic solvent tolerance in Escherichia coli, Rei Watanabe, Noriyuki Doukyu, AMB EXPRESS, AMB EXPRESS, 2, Refereed
  • Structural characterization of the organic solvent-stable cholesterol oxidase from Chromobacterium sp DS-1, Martin Sagermann, Akashi Ohtaki, Keith Newton, Noriyuki Doukyu, JOURNAL OF STRUCTURAL BIOLOGY, JOURNAL OF STRUCTURAL BIOLOGY, 170, (1) 32 - 40, 04 , Refereed
  • Enhancement of the aspartame precursor synthetic activity of an organic solvent-stable protease, Hiroyasu Ogino, Shotaro Tsuchiyama, Masahiro Yasuda, Noriyuki Doukyu, PROTEIN ENGINEERING DESIGN & SELECTION, PROTEIN ENGINEERING DESIGN & SELECTION, 23, (3) 147 - 152, 03
  • Organic solvent-tolerant enzymes, Noriyuki Doukyu, Hiroyasu Ogino, BIOCHEMICAL ENGINEERING JOURNAL, BIOCHEMICAL ENGINEERING JOURNAL, 48, (3) 270 - 282, 02 , Refereed
  • Characteristics and biotechnological applications of microbial cholesterol oxidases, Noriyuki Doukyu, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 83, (5) 825 - 837, 07
  • Cloning, sequence analysis, and expression of a gene encoding Chromobacterium sp DS-1 cholesterol oxidase, Noriyuki Doukyu, Kanpei Shibata, Hiroyasu Ogino, Martin Sagermann, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 82, (3) 479 - 490, 03
  • A maltooligosaccharide-forming amylase gene from Brachybacterium sp strain LB25: Cloning and expression in Escherichia coli, Noriyuki Doukyu, Wataru Yamagishi, Hirokazu Kuwahara, Hiroyasu Ogino, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 72, (9) 2444 - 2447, 09
  • Purification and characterization of Chromobacterium sp DS-1 cholesterol oxidase with thermal, organic solvent, and detergent tolerance, Noriyuki Doukyu, Kanpei Shibata, Hiroyasu Ogino, Martin Sagermann, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 80, (1) 59 - 70, 08
  • Refolding of a recombinant organic solvent-stable lipase, which is overexpressed and forms an inclusion body, and activation with lipase-specific foldase, Hiroyasu Ogino, Sosuke Inoue, Rieko Akagi, Masahiro Yasuda, Noriyuki Doukyu, Kosaku Ishimi, BIOCHEMICAL ENGINEERING JOURNAL, BIOCHEMICAL ENGINEERING JOURNAL, 40, (3) 507 - 511, 07
  • Characterization of recombinant glyoxylate reductase from thermophile Thermus thermophilus HB27, Hiroyasu Ogino, Hitoshi Nakayama, Hideyasu China, Takuya Kawata, Noriyuki Doukyu, Masahiro Yasuda, BIOTECHNOLOGY PROGRESS, BIOTECHNOLOGY PROGRESS, 24, (2) 321 - 325, 03
  • Cloning and expression of gene, and activation of an organic solvent-stable lipase from Pseudomonas aeruginosa LST-03, Hiroyasu Ogino, Yoshikazu Katou, Rieko Akagi, Takashi Mimitsuka, Shinichi Hiroshima, Yuichi Gemba, Noriyuki Doukyu, Masahiro Yasuda, Kosaku Ishimi, Haruo Ishikawa, EXTREMOPHILES, EXTREMOPHILES, 11, (6) 809 - 817, 11
  • Purification and characterization of a maltooligosaccharide-forming amylase that improves product selectivity in water-miscible organic solvents, from dimethylsulfoxide-tolerant Brachybacterium sp strain LB25, Noriyuki Doukyu, Wataru Yamagishi, Hirokazu Kuwahara, Hiroyasu Ogino, Noritake Furuki, EXTREMOPHILES, EXTREMOPHILES, 11, (6) 781 - 788, 11
  • Effect of exchange of amino acid residues of the surface region of the PST-01 protease on its organic solvent-stability, Hiroyasu Ogino, Takeshi Uchiho, Noriyuki Doukyu, Masahiro Yasuda, Kosaku Ishimi, Haruo Ishikawa, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 358, (4) 1028 - 1033, 07
  • Peptide synthesis of aspartame precursor using organic-solvent-stable PST-01 protease in monophasic aqueous - Organic solvent systems, Shotaro Tsuchiyama, Noriyuki Doukyu, Masahiro Yasuda, Kosaku Ishimi, Hiroyasu Ogino, BIOTECHNOLOGY PROGRESS, BIOTECHNOLOGY PROGRESS, 23, (4) 820 - 823, 07
  • Discovery of glpC, an organic solvent tolerance-related gene in Escherichia coli, using gene expression profiles from DNA microarrays, K Shimizu, S Hayashi, T Kako, M Suzuki, N Tsukagoshi, N Doukyu, T Kobayashi, H Honda, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 71, (2) 1093 - 1096, 02
  • Discovery of glpC, an organic solvent tolerance-related gene in Escherichia coli, using gene expression profiles from DNA microarrays, K Shimizu, S Hayashi, T Kako, M Suzuki, N Tsukagoshi, N Doukyu, T Kobayashi, H Honda, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 71, (2) 1093 - 1096, 02
  • Convenient and sensitive evaluation of a superoxide anion-generating reagent methyl viologen by Escherichia coli harboring a soxS':: gfp reporter plasmid, A Shibuya, N Tsukagoshi, Ohtsu, I, N Dokyu, R Aono, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 68, (12) 2637 - 2639, 12
  • Transcriptional analysis of the ostA/imp gene involved in organic solvent sensitivity in Escherichia coli, Ohtsu, I, N Kakuda, N Tsukagoshi, N Dokyu, H Takagi, M Wachi, R Aono, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 68, (2) 458 - 461, 02
  • Crystallization and preliminary X-ray crystallographic studies on the class II cholesterol oxidase from Burkholderia cepacia containing bound flavin, R Aunpad, SP Muench, PJ Baker, S Sedelnikova, W Panbangred, N Doukyu, R Aono, DW Rice, ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 58, 2182 - 2183, 12
  • Isolation of an Acinetobacter sp ST-550 which produces a high level of indigo in a water-organic solvent two-phase system containing high levels of indole, N Doukyu, T Nakano, Y Okuyama, R Aono, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 58, (4) 543 - 546, 03
  • Isolation of an Acinetobacter sp ST-550 which produces a high level of indigo in a water-organic solvent two-phase system containing high levels of indole, N Doukyu, T Nakano, Y Okuyama, R Aono, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 58, (4) 543 - 546, 03
  • Lithocholic acid side-chain cleavage to produce 17-keto or 22-aldehyde steroids by Pseudomonas putida strain ST-491 grown in the presence of an organic solvent, diphenyl ether, Y Suzuki, N Doukyu, R Aono, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 62, (11) 2182 - 2188, 11

Misc

  • Discovery of glpC, an organic solvent tolerance-related gene in Escherichia coli, using gene expression profiles from DNA microarrays, K Shimizu, S Hayashi, T Kako, M Suzuki, N Tsukagoshi, N Doukyu, T Kobayashi, H Honda, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 71, (2) 1093 - 1096,   2005 02 , Gene expression profiles were collected from Escherichia coli strains (OST3410, TK33, and TK31) before and after exposure to organic solvents, and the six genes that showed higher gene expression were selected. Among these genes, glpC encoding the anaerobic glycerol-3-phosphate dehydrogenase subunit C remarkably increased the organic solvent tolerance.
  • Time-course data analysis of gene expression profiles reveals purR Regulation concerns in organic solvent tolerance in Escherichia coli., J. Biosci. Bioeng., 99:72-74.,   2005
  • Convenient and sensitive evaluation of a superoxide anion-generating reagent methyl viologen by Escherichia coli harboring a soxS'::gfp reporter plasmid., Biosci Biotechnol Biochem., 68(12):2637-9.,   2004
  • Transcriptional analysis of the ostA/imp gene involved in organic solvent sensitivity in Escherichia coli., Biosci. Biotechnol. Biochem., 68: 458-461,   2004
  • Isolation of Paenibacillus illinoisensis which produces organic solvent tolerant-cyclodextrin glucanotransferase., Biosci. Biotechnol. Biochem., 67: 334-340,   2003
  • Indigo production by Escherichia coli carrying phenol hydroxylase gene from Acinetobacter sp. strain ST-550 in a water-organic solvent two phase system., Appl. Microbiol.Biotechnol., 60:720-725,   2003
  • Isolation of an Acinetobacter sp ST-550 which produces a high level of indigo in a water-organic solvent two-phase system containing high levels of indole, N Doukyu, T Nakano, Y Okuyama, R Aono, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 58, (4) 543 - 546,   2002 03 , Acinetobacter sp. strain ST-550 was isolated from fumus soil as an efficient indigo producer in the presence of organic solvent. The minimum inhibitory concentration of indole was 0.4 mg/ml for ST-550. ST-550 produced only a small amount of indigo (less than 0.01 mug/ml) when grown in the presence of indole at concentrations of 0.05 to 0.3 mg/ml without any organic solvent. However, ST-550 produced indigo effectively when grown in the presence of a large: volume of diphenylmethane and a high level of indole; optimized conditions were 3 ml of a medium containing 0.3 ml diphenylmethane and 2.7 mg indole. Under these conditions, ST-550 produced 0.88 mg indigo (292 mug/ml medium).
  • Crystallization and preliminary X-ray crystallographic studies on the class II cholesterol oxidase containing bound flavin from Burkholderia cepacia., Acta. Crystallogr. D., 58:2182-2183,   2002
  • Cloning, sequence analysis and expression of a gene encoding an organic solvent- and detergent-tolerant cholesterol oxidase of Burkholderia cepacia strain ST-200, N Doukyu, R Aono, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 57, (1-2) 146 - 152,   2001 10 , Burkholderia cepacia strain ST-200 produces an extracellular cholesterol oxidase which is stable and highly active in the presence of organic solvents, This cholesterol oxidase produces 6 beta -hydroperoxycholest-4-en-3-one from cholesterol, with the consumption of two moles of O-2 and the formation of one mole of H2O2. The structural gene encoding the cholesterol oxidase was cloned and sequenced. The primary translation product was predicted to be 582 amino acid residues. The mature product is composed of 539 amino acid residues and is preceded by a signal sequence of 43 residues. The cloned gene was expressed as an active product in Escherichia coli and the product was localized in the periplasmic space. The cholesterol oxidase produced from E. coli was purified to homogeneity from the periplasmic fraction. The purified enzyme was highly stable in the presence of various organic solvents or detergents, as compared with the commercially available cholesterol oxidases tested.
  • Two moles of O2 consumption and one mole of H2O2 formation during cholesterol peroxidation with cholesterol peroxidation with cholesterol oxidase with from Pseudomonas sp. strain ST-200, Biochem. J., 341, (3) 621 - 627,   1999
  • Effects of organic solvents on indigo formation by Pseudomonas sp. strain ST-200 grown with high levels of indole, N Doukyu, T Arai, R Aono, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 62, (6) 1075 - 1080,   1998 06 , The indole tolerance level of Pseudomonas sp. strain ST-200 was 0.25 mg/ml. The level was raised to 4 mg/ml when diphenylmethane was added to the medium to 20% by volume. ST-200 grown in this two-phase culture system containing indole (1 mg/ml) and diphenylmethane (0.2 ml/ml) produced a water-soluble yellow pigment, isatic acid, and two water-insoluble and diphenylmethane-soluble pigments, blue indigo and purple indirubin. The amounts of the water-insoluble pigments corresponded to 0.5% (indigo) and 0.2% (indirubin) of the indole added to the medium. Of the conditions tried, indigo and indirubin were formed only when ST-200 was grown in the two-phase system overlaid with organic solvents with appropriate polarity.
  • Purification of extracellular cholesterol oxidase with high activity in the presence of organic solvents from Pseudomonas sp. strain ST-200, Appl. Environ. Microbiol., 64,   1998
  • Lithocholic acid side-chain cleavage to produce 17-keto or 22-aldehyde steroids by Pseudomonas putida strain ST-491 grown in the presence of organic solvent, diphenyl ether, Biosci. Biotechnol. Biochem., 62:2182-2188,   1998
  • Biodegradation of indole at high concentration by persolvent fermentation with Pseudomonas sp. ST-200., Extremophiles, 1:100-105,   1997
  • Aono. Control with organic solvents of efficiency of persolvent fermentation by Pseudomonas sp. strain ST-200., Biosci. Biotech. Biochem., 60:1612-1616,   1996
  • Stereospecific oxidation of 3-hydroxysteroids by persolvent fermentation with Pseudomonas sp. ST-200., Biosci. Biotech. Biochem., 60:1146-1151,   1996
  • Oxidative Bioconversion of cholesterol by Pseudomonas sp. strain ST-200 in a water-organic solvent two-phase system., Appl. Environ. Microbiol., 60:2518-2523,   1994

Conference Activities & Talks

  • 2, DOUKYU Noriyuki, DN000063,   2014 11
  • 1, DOUKYU Noriyuki, DN000063,   2014 11
  • 3, DOUKYU Noriyuki, DN000063,   2013 10

Patents

  • PCT/JP2009/052930
  • 特願2008-39355
  • 2003-1068

Research Grants & Projects

  • Organic solvent-resistant enzyme
  • Indigo formation in the presence of organic solvent by a microorganism
  • Cholesterol conversion in the presence of organic solvent by a microorganism