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Takahiro Hosoya

    Department of Nutritional Sciences　Associate Professor
    Course of Food and Nutritional Sciences　Associate Professor
Last Updated :2025/04/19

Researcher Information

Research funding number

  • 30506572

J-Global ID

Research Interests

  • 食品機能学   食品分析学   天然物化学   

Research Areas

  • Life sciences / Food sciences

Academic & Professional Experience

  • 2018/04 - Today  Toyo University食環境科学部

Published Papers

MISC

Industrial Property Rights

Research Grants & Projects

  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2015/04 -2018/03 
    Author : Kumazawa Shigenori
     
    Propolis is a natural resinous substance collected by honeybees from buds and exudates of certain trees and plants to protect their beehive from enemies. It is used as folk medicine in many regions of the world and has been reported to display various biological activities. In this study, chemical and biological methods were applied to evaluate the functions of propolis. In particular, chemical analyses of the propolis from tropic and sub tropic areas such as Senegal, Indonesia and Thailand were carried out. We identified several stilbene compounds from Senegalese propolis. Further the anti-inflammatory potency of Senegalese propolis was higher than that of other propolis that had been previously reported. On the other hand, we clarified that artepillin C from Brazilian propolis induces brown-like adipocyte formation and nymphaeol-C suppresses the expression of fibroblast growth factor 18.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2013/04 -2015/03 
    Author : HOSOYA Takahiro
     
    Apoptosis inhibitor of macrophage (AIM) is a secreted protein, which is produced and secreted specifically by macrophages. The expression in vivo is markedly increased with obesity progression in mice. Thus, regulation of AIM could be therapeutically applicable for metabolic syndrome. Since the AIM decreases the accumulated lipid droplet in adipocytes, we screened of food-derived constituents for AIM up-regulation effects. We used real time RT-PCR methods with 96-well plate format for the screening of 25 food extracts and 30 food derived constituents. Unfortunately, we could not find the up-regulated AIM sample in any food extracts. Next, we tested the the food derived constituents at 10 μM and 100 μM on AIM expression in J774.1 macrophage-like cells. Among them, quercetin (17), 8-prenylnarigenin (21), and isoxanthohumol (24) exhibited the up-regulation effects of AIM expression in J774.1 cells at 100 μM with high cell viability.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2009 -2010 
    Author : HOYOYA Takahiro
     
    Pokemon (POK erythroid myeloid ontogenic factor ; LRF, FBI1, ZBTB7A) is a transcriptional suppressor. It is reported that the overexpression of Pokemon in lung cancer cell caused oncogenesis or maintain of the cancer. To search for inhibitors of the Pokemon signaling pathway, new cell-based assay systems were developed and screening of natural product or natural resources have been performed.
  • 日本学術振興会：科学研究費助成事業
    Date (from‐to) : 2006 -2007 
    Author : 細谷 孝博
     
    最近の研究で,胚発生において重要な役割をしているヘッジホックシグナル(Hh)伝達経路が,種々の癌細胞で異常亢進し,腫瘍の発生や拡大に寄与しているとの報告がされた.これら癌細胞では,Hhシグナル伝達経路を遮断することが有効な治療法の一つとして考えられており,本研究では,Hhシグナルの最下流に位置する転写因子GLIによる転写活性を阻害する化合物を,天然資源より得,新たなタイプのHh阻害剤を提案することにした. まず,目的にあったアッセイ系を,細胞を用いたレポーターアッセイを基本にして構築した.ヒト正常皮膚細胞(HaCaT細胞)に標的である転写因子GLI1を過剰に発現させ,GLI binding siteを有するレポータープラスミドでその転写活性を,ルシフェリン/ルシフェラーゼ反応による発光量で測定するシステムである.構築したアッセイ系において,スクリーニングの最適条件を検討し,スクリーニングのプロトコルを作成した. 作成したスクリーニングシステムにて,研究室保有の天然物ライブラリーをスクリーニングしたところ,zerumbone (1), zerumbone epoxide (2), staurosporinone (3), 6-hydroxystaurosporinone (4), arcyriaflavin C (5), 5,6-dihydroxyarcyriaflavin A (6)を同定した.また,天然資源(植物,放線菌,生薬等)をスクリーニングした結果,Physalis minimaのメタノール抽出物にGLI1転写阻害活性を見出し,P. minimaよりphysalin F(7)およびB(8)を活性化合物として単離した. 構築したアッセイ系で同定した化合物の中で,1,3,7および8について,Hhシグナル伝達経路の異常亢進が原因で増殖が認められている膵臓癌細胞株(PANC1)およびヒト前立腺癌細胞株(DU145)におけるHhシグナルに関与する遺伝子発現を確認した.その結果,構築したアッセイ系で同定した化合物は,PANC1およびDU145細胞に対して,細胞毒性を示し,これら細胞株で,Hhシグナルに関与する遺伝子(Ptch, Bc12等)の発現を抑制することが判明し,天然資源よりGLI転写阻害活性化合物の同定に成功した.

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