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Masahiro Ito

Faculty
Department of Life Sciences
Bio-Nano Electronics Research Center
Course of Life Sciences
PositionProfessor
Mail
HomepageURL
Birthday
Last Updated :2019/10/04

Researcher Profile and Settings

Association Memberships

  • American society for Microbiology
  • AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY
  • International society for Extremophiles
  • THE JAPANESE SOCIETY FOR EXTREMOPHILES
  • JAPAN SOCIETY FOR BIOSICENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY
  • The Biophysical Society of Japan

Committee Memberships

  •   2015  - 現在, Applied and Environmental Microbiology, Editorial Board Member
  •   2013 04  - 現在, Center for Academic Research Promotion, Deputy-Directors
  •   2013 01  - 現在, Japanese Society for Extremophiles, General affairs secretary
  •   2008 08  - 2010 07 , Ministry of Education, Culture, Sports, Science and Technology (MEXT), Program officer

Academic & Professional Experience

  •   2009 04  - 現在, Toyo University, Factory of Life Sciences, Professor
  •   2001 04  - 2009 03 , Toyo University, Factory of Life Sciences, Associate professor
  •   1997 04  - 2001 03 , Toyo University, Factory of Life Sciences, Lecturer
  •   1994 04  - 1997 03 , Mount Sinai School of Medicine, Department of Biochemistry, postdoctoral researcher

Research Activities

Research Areas

  • Biological Science, Molecular biology
  • Agricultural chemistry, Applied microbiology
  • Biological Science, Biophysics

Research Interests

    Bacillus, Bacillus pseudofirmus

Published Papers

  • A Hydrophobic Small Protein, BpOF4_01690, Is Critical for Alkaliphily of Alkaliphilic Bacillus pseudofirmus OF4, Tetsuaki Takahashi, Terry Ann Krulwich, Masahiro Ito, Frontiers in Microbiology, 9, 08 , Refereed
  • The Surface Layer Homology Domain-Containing Proteins of Alkaliphilic Bacillus pseudofirmus OF4 Play an Important Role in Alkaline Adaptation via Peptidoglycan Synthesis., Masahiro Ito, Frontiers in Microbiology, 9, 05 , Refereed
  • Mrp Antiporters Have Important Roles in Diverse Bacteria and Archaea. , Masahiro Ito, Masato Morino, Terry Ann Krulwich, Frontiers in Microbiology, 8, 11 , Refereed
  • Ion Selectivity of the Flagellar Motors Derived from the Alkaliphilic Bacillus and Paenibacillus Species, Yuka Takahashi, Masahiro Ito, Methods in Molecular Biology, 1593, 297 - 303, 04 , Refereed, Invited
  • Load-and polysaccharide-dependent activation of the Na+-type MotPS stator in the Bacillus subtilis flagellar motor., Terahara, N., Noguchi, Y., Nakamura, S., Kamiike, N., Ito, M., Namba, K., Minamino, T., Scientific Reports, 7, 04 , Refereed
  • Nonconventional cation-coupled flagellar motors derived from the alkaliphilic Bacillus and Paenibacillus species, Ito M, Takahashi Y, Extremophiles, 21, (1) 3 - 14, 01 , Refereed
  • Differences in the phenotypic effects of mutations in homologous MrpA and MrpD subunits of the multi-subunit Mrp-type Na+/H+ antiporter, Morino M, Ogoda S, Krulwich TA, Ito M, Extremophiles, 21, (1) 51 - 64, 01 , Refereed
  • A novel type bacterial flagellar motor that can use divalent cations as a coupling ion, Riku Imazawa, Yuka Takahashi, Motohiko Sano, Wataru Aoki, Masahiro Ito, Scientific Reports, 6, e19773 - g0000197042, 01 , Refereed
  • Draft Genome Sequence of Methylobacterium sp. ME121, isolated from soil as a mixed single colony with Kaistia sp. 32K, Fujinami S, Takeda-Yano K, Onodera T, Satoh K, Shimizu T, Wakabayashi Y, Narumi I, Nakamura A, Ito M, Genome Announcements, 3, (5) , 09 , Refereed
  • Genome Sequence of the Deep-Sea Denitrifier Pseudomonas sp. Strain MT-1 isolated from the Mariana Trench, Fujinami S, Oikawa Y, Araki T, Shinmura Y, Midorikawa R, Ishizaka H, Kato C, Horikoshi K, Ito M, and Tamegai H., Genome Announcements, 2, (6) , 12 , Refereed
  • Ionic selectivity and thermal adaptations within the voltage-gated sodium channel family of alkaliphilic Bacillus, DeCaen PG, Takahashi Y, Krulwich TA, Ito M, Clapham DE., eLife, 3, 11 , Refereed
  • Draft Genome Sequence of Bacillus alcalophilus AV1934, a Classic Alkaliphile Isolated from Human Feces in 1934., Attie O, Jayaprakash A, Shah H, Paulsen IT, Morino M, Takahashi Y, Narumi I, Sachidanandam R, Satoh K, Ito M, and Krulwichc TA., Genome Announcements, 2, (6) , 11 , Refereed
  • Suppressor mutants from MotB-D24E and MotS-D30E in the flagellar stator complex of Bacillus subtilis., Takahashi Y., Koyama K., Ito M., Journal of General and Applied Microbiology, 60, (4) 131 - 139, 10 , Refereed
  • Draft Genome Sequence of Calcium-dependent Paenibacillus sp. Strain TCA20, Isolated from a Hot Spring Containing a High Concentration of Calcium Ions., Fujinami S, Takeda-Yano K, Onodera T, Satoh K, Sano M, Takahashi Y, Narumi I, Ito M., Genome Announcements, 2, (5) , 09 , Refereed
  • Draft Genome Sequence of Potassium-Dependent Alkaliphilic Bacillus sp. Strain TS-2 isolated from a jumping spider., Fujinami S., Takeda K., Onodera T., Satoh S., Sano M., Narumi I., Ito M., Genome Announcements, 2, (3) , 05 , Refereed
  • Mutational analysis of charged residues in the cytoplasmic loops of MotA and MotP in the Bacillus subtilis flagellar motor., Yuka Takahashi, Masahiro Ito, Journal of Biochemistry, 156, (4) 211 - 220, 04 , Refereed
  • Purification and functional reconstitution of a seven-subunit mrp-type na+/h+ antiporter., Morino M, Suzuki T, Ito M, Krulwich TA, Journal of bacteriology, 196, 28 - 35, 01 , Refereed
  • Draft Genome Sequence of Sodium-Independent Alkaliphilic Microbacterium sp. Strain TS-1., Fujinami S, Takeda K, Onodera T, Satoh K, Sano M, Narumi I, Ito M, Genome announcements, 1, 12 , Refereed
  • Functional expression of the multi-subunit type calcium/proton antiporter from Thermomicrobium roseum., Morino M, Ito M, FEMS microbiology letters, 335, 26 - 30, 10 , Refereed
  • A Bacillus flagellar motor that can use both Na+ and K+ as a coupling ion is converted by a single mutation to use only Na+., Terahara N, Sano M, Ito M, PloS one, 7, Refereed
  • Genome of alkaliphilic Bacillus pseudofirmus OF4 reveals adaptations that support the ability to grow in an external pH range from 7.5 to 11.4., Janto B, Ahmed A, Ito M, Liu J, Hicks DB, Pagni S, Fackelmayer OJ, Smith TA, Earl J, Elbourne LD, Hassan K, Paulsen IT, Kolstø AB, Tourasse NJ, Ehrlich GD, Boissy R, Ivey DM, Li G, Xue Y, Ma Y, Hu FZ, Krulwich TA, Environmental microbiology, 13, 3289 - 3309, 12 , Refereed
  • The relationship between a coiled morphology and Mbl in alkaliphilic Bacillus halodurans C-125 at neutral pH values., Fujinami S, Sato T, Ito M, Extremophiles : life under extreme conditions, 15, 587 - 596, 09 , Refereed
  • Single site mutations in the hetero-oligomeric Mrp antiporter from alkaliphilic Bacillus pseudofirmus OF4 that affect Na+/H+ antiport activity, sodium exclusion, individual Mrp protein levels, or Mrp complex formation., Morino M, Natsui S, Ono T, Swartz TH, Krulwich TA, Ito M, The Journal of biological chemistry, 285, 30942 - 30950, 10 , Refereed
  • Motility and chemotaxis in alkaliphilic Bacillus species., Fujinami S, Terahara N, Krulwich TA, Ito M, Future microbiology, 4, 1137 - 1149, 11 , Refereed
  • Cation/proton antiporter complements of bacteria: why so large and diverse?, Krulwich TA, Hicks DB, Ito M, Molecular microbiology, 74, 257 - 260, 10 , Refereed
  • Characterization of Bacillus subtilis YfkE (ChaA): a calcium-specific Ca2+/H+ antiporter of the CaCA family., Fujisawa M, Wada Y, Tsuchiya T, Ito M, Archives of microbiology, 191, 649 - 657, 08 , Refereed
  • Mutations alter the sodium versus proton use of a Bacillus clausii flagellar motor and confer dual ion use on Bacillus subtilis motors., Terahara N, Krulwich TA, Ito M, Proceedings of the National Academy of Sciences of the United States of America, 105, 14359 - 14364, 09 , Refereed
  • Single gene deletions of mrpA to mrpG and mrpE point mutations affect activity of the Mrp Na+/H+ antiporter of alkaliphilic Bacillus and formation of hetero-oligomeric Mrp complexes., Morino M, Natsui S, Swartz TH, Krulwich TA, Ito M, Journal of bacteriology, 190, 4162 - 4172, 06 , Refereed
  • The voltage-gated Na+ channel NaVBP co-localizes with methyl-accepting chemotaxis protein at cell poles of alkaliphilic Bacillus pseudofirmus OF4., Fujinami S, Sato T, Trimmer JS, Spiller BW, Clapham DE, Krulwich TA, Kawagishi I, Ito M, Microbiology (Reading, England), 153, 4027 - 4038, 12 , Refereed
  • Three two-component transporters with channel-like properties have monovalent cation/proton antiport activity., Fujisawa M, Ito M, Krulwich TA, Proceedings of the National Academy of Sciences of the United States of America, 104, 13289 - 13294, 08 , Refereed
  • Catalytic properties of Staphylococcus aureus and Bacillus members of the secondary cation/proton antiporter-3 (Mrp) family are revealed by an optimized assay in an Escherichia coli host., Swartz TH, Ito M, Ohira T, Natsui S, Hicks DB, Krulwich TA, Journal of bacteriology, 189, 3081 - 3090, 04 , Refereed
  • Na(+) and flagella-dependent swimming of alkaliphilic Bacillus pseudofirmus OF4: a basis for poor motility at low pH and enhancement in viscous media in an "up-motile" variant., Fujinami S, Terahara N, Lee S, Ito M, Archives of microbiology, 187, 239 - 247, 03 , Refereed
  • An intergenic stem-loop mutation in the Bacillus subtilis ccpA-motPS operon increases motPS transcription and the MotPS contribution to motility., Terahara N, Fujisawa M, Powers B, Henkin TM, Krulwich TA, Ito M, Journal of bacteriology, 188, 2701 - 2705, 04 , Refereed
  • The activity profile of the NhaD-type Na+(Li+)/H+ antiporter from the soda Lake Haloalkaliphile Alkalimonas amylolytica is adaptive for the extreme environment., Liu J, Xue Y, Wang Q, Wei Y, Swartz TH, Hicks DB, Ito M, Ma Y, Krulwich TA, Journal of bacteriology, 187, 7589 - 7595, 11 , Refereed
  • The Mrp system: a giant among monovalent cation/proton antiporters?, Swartz TH, Ikewada S, Ishikawa O, Ito M, Krulwich TA, Extremophiles : life under extreme conditions, 9, 345 - 354, 10 , Refereed
  • NhaK, a novel monovalent cation/H+ antiporter of Bacillus subtilis., Fujisawa M, Kusumoto A, Wada Y, Tsuchiya T, Ito M, Archives of microbiology, 183, 411 - 420, 09 , Refereed
  • Properties of motility in Bacillus subtilis powered by the H+-coupled MotAB flagellar stator, Na+-coupled MotPS or hybrid stators MotAS or MotPB., Ito M, Terahara N, Fujinami S, Krulwich TA, Journal of molecular biology, 352, 396 - 408, 09 , Refereed
  • MotPS is the stator-force generator for motility of alkaliphilic Bacillus, and its homologue is a second functional Mot in Bacillus subtilis., Ito M, Hicks DB, Henkin TM, Guffanti AA, Powers BD, Zvi L, Uematsu K, Krulwich TA, Molecular microbiology, 53, 1035 - 1049, 08 , Refereed
  • The voltage-gated Na+ channel NaVBP has a role in motility, chemotaxis, and pH homeostasis of an alkaliphilic Bacillus., Ito M, Xu H, Guffanti AA, Wei Y, Zvi L, Clapham DE, Krulwich TA, Proceedings of the National Academy of Sciences of the United States of America, 101, 10566 - 10571, 07 , Refereed
  • Modulation of the K+ efflux activity of Bacillus subtilis YhaU by YhaT and the C-terminal region of YhaS., Fujisawa M, Wada Y, Ito M, FEMS microbiology letters, 231, 211 - 217, 02 , Refereed
  • Mutational loss of a K+ and NH4+ transporter affects the growth and endospore formation of alkaliphilic Bacillus pseudofirmus OF4., Wei Y, Southworth TW, Kloster H, Ito M, Guffanti AA, Moir A, Krulwich TA, Journal of bacteriology, 185, 5133 - 5147, 09 , Refereed
  • Decrease in cytoplasmic pH-homeostastatic activity of the alkaliphile Bacillus lentus C-125 by a cell wall defect., Ito M, Aono R, Bioscience, biotechnology, and biochemistry, 66, 218 - 220, 01 , Refereed

Misc

  • 世界初:2価陽イオンで駆動するべん毛モーターCa2+やMg2+でもべん毛は回転する, Masahiro Ito, kagaku to seibutsu, 55, (4) 240 - 241,   2017 03 , 査読有り, 招待有り
  • 13.好アルカリ性細菌の高アルカリ性環境適応機構, Yuka Takahashi, Masahiro Ito, Nihon nyusankin gakkai shi, 25, (3) 177 - 177,   2014 11 , 査読有り, 招待有り
  • Alkaliphilic Microorganisms : Lessons from Their Adaptations(Papers presented at the Seminar, "Biological mechanism of stress perception"), ITO Masahiro, Cryobiology and cryotechnology, 57, (1) 25 - 32,   2011 04 15 , Alkaliphiles, which are capable of growth in an extremely alkaline environment, serve as a model system to understand the physiological mechanisms allowing microbial growth under extreme environmental conditions. The internal pH of alkaliphiles is kept 2 units lower than the external pH 10 or higher. In alkaliphilic Bacillus, Na^+-dependent pH homeostasis involves the Na^+/H^+ antiport activities as well as Na^+ re-entry routes. A predominant role was evident for the NaChBac (voltage-dependent) Na channel of alkaliphilic B. pseudofirmus OF4 at sub-optimal [Na^+]. NaChBac mutants exhibit a s...
  • Single Stator Complex of Bacterial Flagellar Motor Can Be Use Both H^+ and Na^+ for Stator Force Generator in Alkaliphilic Bacillus and Confer Dual Ion Use on Bacillus subtilis Motors, ITO Masahiro, TERAHARA Naoya, KAGAKU TO SEIBUTSU, 47, (7) 473 - 479,   2009 07 01
  • Molecular basis and swimming properties of a natural dual hybrid flagellar motor of Bacillus subtilis, TERAHARA Naoya, ITO Masahiro, Bioscience & industry, 66, (7) 366 - 368,   2008 07 01
  • Channel-like two-component transporters that have monovalent-cation/proton antiport activity, FUJISAWA Makoto, ITO Masahiro, Bioscience & industry, 66, (6) 300 - 302,   2008 06 01
  • The Physiological Role of Two Sodium Channels MotPS and Na_vBP of Alkaliphilic Bacillus, ITOH Masahiro, Biophysics, 46, (1) 20 - 25,   2006 01 25 , In alkaliphilic Bacillus, Na^+-dependent pH homeostasis involves antiport activities as well as Na^+ re-entry routes. MotPS that are homologous to MotAB are required for the Na^+-dependent motility of alkaliphilic B. pseudofirmus OF4. Purified and reconstituted MotPS support amiloride analogue-sensitive Na^+ flux. Mutants lacking functional MotPS display no deficit in pH homeostasis in pH shift experiments conducted with sub-optimal added [Na^+] in the absence of solutes whose uptake is coupled to Na^+ re-entry. By contrast, a role was evident for the recently described NaChBac (voltage-dep...

Awards & Honors

  •   2016 12 , The 1st international Conference on Applied Microbiology in Vietnam, Young Scientist Poster Award (1st), Elucidation ion selectivity and identification of ion transport subunits in the Mrp type antiporter
  •   2015 11 , The 16th Annual Meeting of the Japanese society for Extremophiles, Poster award, A functional analysis of stator complex of flagellar motor from Bacillus trypoxylicola
  •   2014 12 , The 15th Annual Meeting of the Japanese society for Extremophiles, Poster award, Elucidation of the differences in Na+ affinity in a neutral pH environment of the Na+-driven stator MotPS in alkaliphilic Bacillus pseudofirmus OF4 and Bacillus subtilis
  •   2014 11 , The 15th Annual Meeting of the Japanese society for Extremophiles, Poster award, Identification for a factor affecting of stimulation of motility in Methylobacterium sp. 121
  •   2014 09 , International Congress on Extremophiles 2014, ISE Young Scientist Poster Award , The clarification of the differences between alkaliphilic and neutralophilic Bacillus influx control of Na+ near neutral pH in the Na+-driven flagellar motor stator MotPS
  •   2013 03 , Blue Earth 2013 (JAMSTEC), Young Scientist Poster Award , Analytical results of microbiological diversity in the seismogenic zone (Japan trench) sediment at 3 months and 17 months after Tohoku Region Pacific Offshore Earthquake occurred
  •   2012 11 , The 2012 Annual Meeting of the Kanto Branch of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Poster award, Elucidation of rotation mechanism of hybrid biomotor that can utilize two types of coupling ions
  •   2012 06 , Toyo University, Inoue Enryo Prize, Research on Hybrid Bacterial Flagellar Motor
  •   2011 11 , The 12th Annual Meeting of the Japanese society for Extremophiles, Poster award, A novel K+-stimulated flagellar motor from alkaliphilic Bacillus alcalophilus.
  •   2011 10 , The 2011 Annual Meeting of the Kanto Branch of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Poster award, Identification of amino acid residues in the flagellar stator involved in the reduction of motility under the neutral environment of the alkalophilic Bacillus pseudofirmus OF4
  •   2011 03 , the Japan Society for the Promotion of Science, JSPS Prize, Properties of Bacterial Flagellar Motor Powered by Hybrid Stators
  •   2009 11 , The 10th Annual Meeting of the Japanese society for Extremophiles, Poster award, Identification and characterization of functional residues in a Multi-subunit type Na+/H+ antiporter Mrp complex from alkaliphilic Bacillus pseudofirmus OF4.
  •   2009 10 , The 10th Annual Meeting of the Japanese society for Extremophiles, Poster award, Identification and analysis of amino acid residues important for the rotation mechanism of flagellar stator MotAB, MotPS of Bacillus subtilis
  •   2008 11 , The 9th Annual Meeting of the Japanese society for Extremophiles, Poster award, A conversion of the coupling ion specificity of stators in a Bacillus flagellar motor
  •   2008 06 , The 3rd Japan transporter research association, Outstanding performance award(Oral presentation), A flagellarstator MotAB from alkaliphilic Bacillus clausii can utilize two types of ions as coupling ions
  •   2007 11 , Kanto Branch meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, Young Scientist Award (oral presentation), Bacillus subtilis flagellar motor is a hybrid motor that can utilize two kinds of energy
  •   2007 11 , The 8th Annual Meeting of the Japanese society for Extremophiles, Poster award, The visualization of the voltage-gated Na+ channel of Alkaliphilic Bacillus pseudofirmus OF4 by using fluorescent protein fusion.
  •   2005 11 , the Japan Society for Extremophiles, Young Scientist Award
  •   2004 11 , The 5th Annual Meeting of the Japanese society for Extremophiles, Poster award
  •   2002 09 , International Society for Extremophiles (ISE), ISE Award

Research Grants & Projects

  • Elucidation of structure-function of unique bacterial flagellar stator complex from Bacillus spp., Ministry of Education, Culture, Sports, Science and Technology, Grants-in-Aid for Scientific Research(基盤研究(B)), Masahiro ITO, At first, we identified that the flagellar motor of B. subtilis was a hybrid motor and it rotated by using both ion motive forces, H+ and Na+, by a single molecular mechanism. Next, the critical charged amino acid residues between the second and third transmembrane cytoplasmic loop region in MotA and MotP subunits of B. subtilis were identified by using site-directed mutagenesis. Moreover, we identified a novel type flagellar motor from alkaliphilic Bacillus alcalophilus which stimulates swimming speed under elevated K+ or Rb+ concentrations. This is the first report that describes aflagellar motor that can use both K+ and Rb+ as coupling ions.
  • The Voltage-Gated Na+ Channel Co-localizes with Methyl-Accepting Chemotaxis Proteins ofAlkaliphile at a Cell Pole, Ministry of Education, Culture, Sports, Science and Technology, Grants-in-Aid for Scientific Research(基盤研究(C)), Masahiro ITO, The bacterial ion channels are useful for the structural analysis of the eukaryotic ion channels. The elucidation of physiologic functions and effecter proteins of the bacterial ion channels is important in research of the ion channels. NavBP is the member of the bacterial voltage-gated Na+ channel superfamily that is found in alkaliphilic Bacillus pseudofirmus OF4. NavBP has recently been shown to have a role in pH homeostasis, motility and chemotaxis. The chemotaxis phenotype of NavBP deletion mutant was particularly striking in that the response of the mutants to chemoattractants was inverted compared to the wild-type strain. Therefore, we have studied to elucidate the relation of the deficit of Na channel and the abnormalities in chemotaxis. We have already reported the following things. First, in the wild-type strain, colocalization of NavBP and the putative chemotaxis receptor McpX was observed at cell pole by immuno fluorescent microscopy (IFM). Second, in the NavBP deletion mutant, although the expression level of McpX did not change mostly, but the localization of that was decreased. Then, in order to observe cellullar localization of NavBP in a live cell, the plasmid which express the NavBP-CFP fusion protein was constructed, the transformation was carried out to NavBP deletion mutant. As a result, polar localization of functional NavBP-CFP was observed. The polar localization of NavBP in alkaliphilic Bacillus pseudofirmus OF4 was strongly suggested. In order for a chemotaxis receptor to function, it is necessary to localized at cell pole. The results suggest interactions between NavBP and McpX that affect their co-localization at cell pole. The inverse chemotaxis phenotype of NavBP deletion mutants may result in part from delocalization of McpX.