Noriyuki Doukyu
| Department of Biological Resources | Professor |
| Bio-Nano Electronics Research Center | Researcher |
| Course of Life Sciences | Professor |
Last Updated :2026/03/20
■Researcher basic information
Degree
- (BLANK), Tokyo Institute of Technology
Research Interests
Research Areas
■Career
Education
■Research activity information
Papers
- Expression and characterization of a thermostable monoacylglycerol lipase from thermophilic Geobacillus kaustophilus
Noriyuki Doukyu; Hayato Ito; Kugako Sugimoto
Preparative Biochemistry & Biotechnology, 04 Jun. 2024 - Expression and characterization of cholesterol oxidase with high thermal and pH stability from Janthinobacterium agaricidamnosum.
Noriyuki Doukyu; Yuuki Ikehata; Taichi Sasaki
Preparative biochemistry & biotechnology, 2023 - Improving E. coli organic solvent tolerance by 1,4-dihydroxy-2-naphthoic acid.
Noriyuki Doukyu; Hideyuki Fujisawa; Ryota Saito
Bioscience, biotechnology, and biochemistry, 22 Jul. 2022 - Improving the organic solvent tolerance of Escherichia coli with vanillin, and the involvement of an AcrAB-TolC efflux pump in vanillin tolerance
Yuuki Ikehata; Noriyuki Doukyu
Journal of Bioscience and Bioengineering, Apr. 2022 - Involvement of catalase and superoxide dismutase in hydrophobic organic solvent tolerance of Escherichia coli
Noriyuki Doukyu; Katsuya Taguchi
AMB Express, Dec. 2021 - Cholesterol oxidase from Rhodococcus erythropolis with high specificity toward β-cholestanol and pytosterols
Noriyuki Doukyu; Makoto Ishikawa
PLOS ONE, 26 Oct. 2020 - Production of styrene oxide from styrene by a recombinant Escherichia coli with enhanced AcrAB-TolC efflux pump level in an aqueous-organic solvent two-phase system
Noriyuki Doukyu; Shinichiro Iida
Bioscience, Biotechnology, and Biochemistry, 02 Jul. 2020, [Reviewed] - Cholesterol oxidase with high catalytic activity from Pseudomonas aeruginosa: Screening, molecular genetic analysis, expression and characterization
Noriyuki Doukyu; Shyou Nihei
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, Jul. 2015, [Reviewed] - Improvement of organic solvent tolerance by disruption of the ion gene in Escherichia coli
Rei Watanabe; Noriyuki Doukyu
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, Aug. 2014, [Reviewed] - Hyper-activation of foldase-dependent lipase with lipase-specific foldase
Hiroyasu Ogino; Sosuke Inoue; Masahiro Yasuda; Noriyuki Doukyu
JOURNAL OF BIOTECHNOLOGY, Jun. 2013, [Reviewed] - Halarchaeum salinum sp nov., a moderately acidophilic haloarchaeon isolated from commercial sea salt
Yuto Yamauchi; Hiroaki Minegishi; Akinobu Echigo; Yasuhiro Shimane; Hirokazu Shimoshige; Masahiro Kamekura; Takashi Itoh; Noriyuki Doukyu; Akira Inoue; Ron Usami
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Mar. 2013, [Reviewed] - Halarchaeum rubridurum sp. nov., a moderately acidophilic haloarchaeon isolated from commercial sea salt samples
Yuto Yamauchi; Hiroaki Minegishi; Akinobu Echigo; Yasuhiro Shimane; Masahiro Kamekura; Takashi Itoh; Moriya Ohkuma; Noriyuki Doukyu; Akira Inoue; Ron Usami
International Journal of Systematic and Evolutionary Microbiology, 2013, [Reviewed] - Novel cholesterol oxidases which were found in the studies of applications of organic solvent-tolerant bacteria.
道久 則之
J. Jpn. Soc. Extremophiles, Mar. 2012 - Improvement in organic solvent tolerance by double disruptions of proV and marR genes in Escherichia coli
N. Doukyu; K. Ishikawa; R. Watanabe; H. Ogino
JOURNAL OF APPLIED MICROBIOLOGY, Mar. 2012, [Reviewed] - Contributions of mutations in acrR and marR genes to organic solvent tolerance in Escherichia coli
Rei Watanabe; Noriyuki Doukyu
AMB EXPRESS, 2012, [Reviewed] - 疎水性有機溶媒に対する大腸菌の耐性機構
道久 則之
生物工学会誌, Jun. 2011 - Structural characterization of the organic solvent-stable cholesterol oxidase from Chromobacterium sp DS-1
Martin Sagermann; Akashi Ohtaki; Keith Newton; Noriyuki Doukyu
JOURNAL OF STRUCTURAL BIOLOGY, Apr. 2010, [Reviewed] - Enhancement of the aspartame precursor synthetic activity of an organic solvent-stable protease
Hiroyasu Ogino; Shotaro Tsuchiyama; Masahiro Yasuda; Noriyuki Doukyu
PROTEIN ENGINEERING DESIGN & SELECTION, Mar. 2010 - Organic solvent-tolerant enzymes
Noriyuki Doukyu; Hiroyasu Ogino
BIOCHEMICAL ENGINEERING JOURNAL, Feb. 2010, [Reviewed] - Characteristics and biotechnological applications of microbial cholesterol oxidases
Noriyuki Doukyu
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, Jul. 2009 - Cloning, sequence analysis, and expression of a gene encoding Chromobacterium sp DS-1 cholesterol oxidase
Noriyuki Doukyu; Kanpei Shibata; Hiroyasu Ogino; Martin Sagermann
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, Mar. 2009 - Cholesterol oxidase with thermal, organic solvent and detergent tolerance
DOUKYU Noriyuki
Bioscience & industry, Dec. 2008 - A maltooligosaccharide-forming amylase gene from Brachybacterium sp strain LB25: Cloning and expression in Escherichia coli
Noriyuki Doukyu; Wataru Yamagishi; Hirokazu Kuwahara; Hiroyasu Ogino
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Sep. 2008 - Purification and characterization of Chromobacterium sp DS-1 cholesterol oxidase with thermal, organic solvent, and detergent tolerance
Noriyuki Doukyu; Kanpei Shibata; Hiroyasu Ogino; Martin Sagermann
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, Aug. 2008 - Refolding of a recombinant organic solvent-stable lipase, which is overexpressed and forms an inclusion body, and activation with lipase-specific foldase
Hiroyasu Ogino; Sosuke Inoue; Rieko Akagi; Masahiro Yasuda; Noriyuki Doukyu; Kosaku Ishimi
BIOCHEMICAL ENGINEERING JOURNAL, Jul. 2008 - Characterization of recombinant glyoxylate reductase from thermophile Thermus thermophilus HB27
Hiroyasu Ogino; Hitoshi Nakayama; Hideyasu China; Takuya Kawata; Noriyuki Doukyu; Masahiro Yasuda
BIOTECHNOLOGY PROGRESS, Mar. 2008 - Cloning and expression of gene, and activation of an organic solvent-stable lipase from Pseudomonas aeruginosa LST-03
Hiroyasu Ogino; Yoshikazu Katou; Rieko Akagi; Takashi Mimitsuka; Shinichi Hiroshima; Yuichi Gemba; Noriyuki Doukyu; Masahiro Yasuda; Kosaku Ishimi; Haruo Ishikawa
EXTREMOPHILES, Nov. 2007 - Purification and characterization of a maltooligosaccharide-forming amylase that improves product selectivity in water-miscible organic solvents, from dimethylsulfoxide-tolerant Brachybacterium sp strain LB25
Noriyuki Doukyu; Wataru Yamagishi; Hirokazu Kuwahara; Hiroyasu Ogino; Noritake Furuki
EXTREMOPHILES, Nov. 2007 - Effect of exchange of amino acid residues of the surface region of the PST-01 protease on its organic solvent-stability
Hiroyasu Ogino; Takeshi Uchiho; Noriyuki Doukyu; Masahiro Yasuda; Kosaku Ishimi; Haruo Ishikawa
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Jul. 2007 - Peptide synthesis of aspartame precursor using organic-solvent-stable PST-01 protease in monophasic aqueous - Organic solvent systems
Shotaro Tsuchiyama; Noriyuki Doukyu; Masahiro Yasuda; Kosaku Ishimi; Hiroyasu Ogino
BIOTECHNOLOGY PROGRESS, Jul. 2007 - Stabilities and conformational transitions of various proteases in the presence of an organic solvent
Hiroyasu Ogino; Yuichi Gemba; Yoshikazu Yutori; Noriyuki Doukyu; Kosaku Ishimi; Haruo Ishikawa
BIOTECHNOLOGY PROGRESS, Jan. 2007 - Purification, characterization, and molecular cloning of organic-solvent-tolerant cholesterol esterase from cyclohexane-tolerant Burkholderia cepacia strain ST-200
Yasuhiko Takeda; Rikizo Aono; Noriyuki Doukyu
EXTREMOPHILES, Aug. 2006 - Discovery of glpC, an organic solvent tolerance-related gene in Escherichia coli, using gene expression profiles from DNA microarrays
K Shimizu; S Hayashi; T Kako; M Suzuki; N Tsukagoshi; N Doukyu; T Kobayashi; H Honda
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Feb. 2005 - Time-course data analysis of gene expression profiles reveals purR regulon concerns in organic solvent tolerance in Escherichia coli
Kazunori Shimizu; Shuhei Hayashi; Noriyuki Doukyu; Takeshi Kobayashi; Hiroyuki Honda
Journal of Bioscience and Bioengineering, 2005 - Convenient and sensitive evaluation of a superoxide anion-generating reagent methyl viologen by Escherichia coli harboring a soxS':: gfp reporter plasmid
A Shibuya; N Tsukagoshi; Ohtsu, I; N Dokyu; R Aono
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Dec. 2004 - Transcriptional analysis of the ostA/imp gene involved in organic solvent sensitivity in Escherichia coli
Ohtsu, I; N Kakuda; N Tsukagoshi; N Dokyu; H Takagi; M Wachi; R Aono
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Feb. 2004 - Isolation of Paenibacillus illinoisensis that produces cyclodextrin glucanotransferase resistant to organic solvents
N Doukyu; H Kuwahara; R Aono
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Feb. 2003 - Indigo production by Escherichia coli carrying the phenol hydroxylase gene from Acinetobacter sp. strain ST-550 in a water-organic solvent two-phase system
N. Doukyu; K. Toyoda; R. Aono
Applied Microbiology and Biotechnology, 2003 - Screening and application of organic solvent resistant-microorganisms
DOUKYU Noriyuki; AONO Rikizo
Bioscience & industry, Jan. 2003 - Crystallization and preliminary X-ray crystallographic studies on the class II cholesterol oxidase from Burkholderia cepacia containing bound flavin
R Aunpad; SP Muench; PJ Baker; S Sedelnikova; W Panbangred; N Doukyu; R Aono; DW Rice
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, Dec. 2002 - Isolation of an Acinetobacter sp ST-550 which produces a high level of indigo in a water-organic solvent two-phase system containing high levels of indole
N Doukyu; T Nakano; Y Okuyama; R Aono
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, Mar. 2002 - 微生物利用の大展開 : 疎水性溶媒耐性微生物-溶媒耐性機構と溶媒重層発酵
エヌ・ティー・エス, 2002 - 特殊環境微生物の検索 : 生物工学実験書 (日本生物工学会編)
培風館, 2002 - Cloning, sequence analysis and expression of a gene encoding an organic solvent- and detergent-tolerant cholesterol oxidase of Burkholderia cepacia strain ST-200
N. Doukyu; R. Aono
Applied Microbiology and Biotechnology, 2001 - Two moles of O2 consumption and one mole of H2O2 formation during cholesterol peroxidation with cholesterol oxidase from Pseudomonas sp. strain ST-200
Noriyuki Doukyu; Rikizo Aono
Biochemical Journal, 01 Aug. 1999 - Lithocholic acid side-chain cleavage to produce 17-keto or 22-aldehyde steroids by Pseudomonas putida strain ST-491 grown in the presence of an organic solvent, diphenyl ether
Y Suzuki; N Doukyu; R Aono
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Nov. 1998 - Purification of extracellular cholesterol oxidase with high activity in the presence of organic solvents from Pseudomonas sp. ST-200.
道久 則之; Doukyu, N; Aono, R
Appl. Environ. Microbiol., Feb. 1998 - Effects of Organic Solvents on Indigo Formation by Pseudomonas sp. strain ST-200 Grown with High Levels of Indole
Noriyuki Doukyu; Tomonori Arai; Rikizo Aono
Bioscience, Biotechnology and Biochemistry, 01 Jan. 1998 - Biodegradation of indole at high concentration by persolvent fermentation with Pseudomonas sp. ST-200
Noriyuki Doukyu; Rikizo Aono
Extremophiles, 1997 - Control with organic solvents of efficiency of persolvent cholesterol fermentation by Pseudomonas sp strain ST-200
N Doukyu; H Kobayashi; H Nakajima; R Aono
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Oct. 1996 - Control with organic solvents of efficiency of persolvent cholesterol fermentation by pseudomonas sp. Strain ST-200
Noriyuki Doukyu; Hideki Kobayashi; Harushi Nakajima; Rikizo Aono
Bioscience, Biotechnology and Biochemistry, 1996 - Stereospecific oxidation of 3β-hydroxysteroids by persolvent fermentation with pseudomonas sp. ST-200
Rikizo Aono; Noriyuki Doukyu
Bioscience, Biotechnology and Biochemistry, 1996 - Oxidative Bioconversion of cholesterol by Pseudomonas sp. strain ST-200 in a water-organic solvent two-phase system.
道久 則之; Aono, R; Doukyu, N; Kobayashi, H; Nakajima, H; Horikoshi, K
Appl. Environ. Microbiol., May 1994
Misc.
- Discovery of glpC, an organic solvent tolerance-related gene in Escherichia coli, using gene expression profiles from DNA microarrays
K Shimizu; S Hayashi; T Kako; M Suzuki; N Tsukagoshi; N Doukyu; T Kobayashi; H Honda
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Feb. 2005 - Time-course data analysis of gene expression profiles reveals purR regulon concerns in organic solvent tolerance in Escherichia coli
Kazunori Shimizu; Shuhei Hayashi; Noriyuki Doukyu; Takeshi Kobayashi; Hiroyuki Honda
Journal of Bioscience and Bioengineering, 2005 - Convenient and sensitive evaluation of a superoxide anion-generating reagent methyl viologen by Escherichia coli harboring a soxS':: gfp reporter plasmid
A Shibuya; N Tsukagoshi; Ohtsu, I; N Dokyu; R Aono
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Dec. 2004 - Transcriptional analysis of the ostA/imp gene involved in organic solvent sensitivity in Escherichia coli
Ohtsu, I; N Kakuda; N Tsukagoshi; N Dokyu; H Takagi; M Wachi; R Aono
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Feb. 2004 - Indigo production by Escherichia coli carrying the phenol hydroxylase gene from Acinetobacter sp strain ST-550 in a water-organic solvent two-phase system
N Doukyu; K Toyoda; R Aono
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, Feb. 2003 - Isolation of paenibacillus illinoisensis that produces cyclodextrin glucanotransferase resistant to organic solvents
Noriyuki Doukyu; Hirokazu Kuwahara; Rikizo Aono
Bioscience, Biotechnology and Biochemistry, 01 Jan. 2003 - Crystallization and preliminary X-ray crystallographic studies on the class II cholesterol oxidase from Burkholderia cepacia containing bound flavin
R Aunpad; SP Muench; PJ Baker; S Sedelnikova; W Panbangred; N Doukyu; R Aono; DW Rice
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, Dec. 2002 - Isolation of an Acinetobacter sp ST-550 which produces a high level of indigo in a water-organic solvent two-phase system containing high levels of indole
N Doukyu; T Nakano; Y Okuyama; R Aono
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, Mar. 2002 - Cloning, sequence analysis and expression of a gene encoding an organic solvent- and detergent-tolerant cholesterol oxidase of Burkholderia cepacia strain ST-200
N Doukyu; R Aono
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, Oct. 2001 - Two moles of O-2 consumption and one mole of H2O2 formation during cholesterol peroxidation with cholesterol oxidase from Pseudomonas sp strain ST-200
N Doukyu; R Aono
BIOCHEMICAL JOURNAL, Aug. 1999 - Lithocholic acid side-chain cleavage to produce 17-keto or 22-aldehyde steroids by Pseudomonas putida strain ST-491 grown in the presence of an organic solvent, diphenyl ether
Y Suzuki; N Doukyu; R Aono
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Nov. 1998 - Effects of organic solvents on indigo formation by Pseudomonas sp. strain ST-200 grown with high levels of indole
N Doukyu; T Arai; R Aono
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Jun. 1998 - Purification of extracellular cholesterol oxidase with high activity in the presence of organic solvents from Pseudomonas sp. strain ST-200
N Doukyu; R Aono
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, May 1998 - Biodegradation of indole at high concentration by persolvent fermentation with Pseudomonas sp. ST-200
N Doukyu; R Aono
EXTREMOPHILES, May 1997 - Control with organic solvents of efficiency of persolvent cholesterol fermentation by Pseudomonas sp strain ST-200
N Doukyu; H Kobayashi; H Nakajima; R Aono
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Oct. 1996 - Stereospecific oxidation of 3 beta-hydroxysteroids by persolvent fermentation with Pseudomonas sp ST-200
R Aono; N Doukyu
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Jul. 1996 - OXIDATIVE BIOCONVERSION OF CHOLESTEROL BY PSEUDOMONAS SP STRAIN-ST-200 IN A WATER-ORGANIC SOLVENT 2-PHASE SYSTEM
R AONO; N DOUKYU; H KOBAYASHI; H NAKAJIMA; K HORIKOSHI
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Jul. 1994
Books and Other publications
- Extremophiles for a Sustainable Future
Joint editor, Future Perspectives for Sustainable Development
Springer, 25 Feb. 2026
9819569699 - Extremophiles for a Sustainable Future
Joint editor, Social Implementation of Methanation Technology Using Methanogenic Archaea
Springer, 25 Feb. 2026
9819569699 - 極限環境微生物の先端科学と社会実装最前線
監修者; 伊藤 政博; 鳴海 一成; 道久 則之, 有機溶媒耐性微生物の有機溶媒耐性メカニズム
株式会社エヌ・ティー・エス, Dec. 2023
9784860438487 - 新版 ビギナーのための微生物実験ラボガイド
中村聡/中島春紫/伊藤政博/道久則之/八波利恵
May 2019 - 極限環境生命―生命の起源を考え、その多様性に学ぶ
道久 則之ほか, Joint work, 9章 有機溶媒耐性微生物
コロナ社, Oct. 2014, Not reviewed - 極限環境生物の産業展開
道久 則之; 今中忠行
シーエムシー出版, Aug. 2012, Not reviewed - 合成生物工学の隆起―有用物質の新たな生産法構築をめざして―
道久 則之; 植田充美監修
シーエムシー出版, Mar. 2012, Not reviewed - Extremophiles Handbook
道久 則之; K. Horikoshi; G. Antranikian; K.O. Ste
Springer, Aug. 2010, Not reviewed - 微生物利用の大展開
道久 則之; 今中忠行; 青野力三
エヌ・ティー・エス, Jul. 2002, Not reviewed - 特殊環境微生物の検索 : 生物工学実験書
道久 則之; 青野力三
培風館, Apr. 2002, Not reviewed
Presentations
- キノン合成中間体による大腸菌の有機溶媒耐性化機構
道久 則之
極限環境生物学会, 08 Dec. 2018 - 高度な有機溶媒耐性を有する大腸菌変異株の解析
道久 則之
極限環境生物学会, 08 Dec. 2018 - 2
DOUKYU Noriyuki
DN000063, Nov. 2014 - 1
DOUKYU Noriyuki
DN000063, Nov. 2014 - 3
DOUKYU Noriyuki
DN000063, Oct. 2013 - Pseudomonas aeruginosa 由来コレステロールオキシダーゼの解析
道久 則之; 道久則之; 二瓶翔
極限環境微生物学会, 01 Dec. 2012 - 「大腸菌の高度有機溶媒耐性化の解明
道久 則之; 渡邉玲; 道久則之
極限環境微生物学会, 01 Dec. 2012 - 大腸菌におけるacrR およびmarR 遺伝子変異による有機溶媒耐性の向上
道久 則之; 渡邉玲; 道久則之
日本農芸化学会 関東支部大会, 27 Oct. 2012 - Improvement in organic solvent-tolerance by double mutations of acrR and marR genes in Escherichia coli
道久 則之; R. Watanabe; N. Doukyu
9th International congress on Extremophiles, 10 Sep. 2012 - Cholesterol oxidases from organic solvent-tolerant bacteria.
道久 則之
The 8th International Congress on Extremophiles., Oct. 2010 - Chromobacterium属細菌由来コレステロールオキシダーゼ遺伝子のクローニングと発現
道久 則之
日本農芸化学会, 29 Mar. 2010 - 有機溶媒耐性大腸菌の溶媒耐性機構と応用
道久 則之
有機溶媒耐性微生物利用技術研究部会シンポジウム(生物工学会), 26 Mar. 2010 - Chromobacterium属細菌由来コレステロールオキシダーゼ遺伝子のクローニング
道久 則之
極限環境微生物学会, Oct. 2009 - Chromobacterium属細菌由来のコレステロールオキシダーゼの精製と解析
道久 則之
日本農芸化学会, Mar. 2009 - Development of a detergent-tolerant cholesterol oxidase for organic reactions in aqueous medium.
道久 則之
The 4th International Symposium on Material Cycling Engineering., Mar. 2009 - Crystal Structure of an Extremely Thermostable Cholesterol Oxidase from a Mesophillic Organism.
道久 則之; A. Ohtaki; K. Newton; N. Doukyu; M. Sagermann
Protein Society 22nd Annual Symposium., Aug. 2008 - Burkholderia cepacia ST-200株由来のコレステロールエステラーゼの解析
道久 則之
極限環境微生物学会, Nov. 2007 - Burkholderia cepacia ST-200株由来のコレステロールエステラーゼの精製と解析
道久 則之
日本農芸化学会2007年度大会, Mar. 2007 - Brachybacterium sp. LB25株の生産するG3アミラーゼの精製と諸性質の解析
道久 則之; 山岸亘
日本農芸化学会, Mar. 2006 - 鹿児島湾若尊プロトカルデラ底泥サンプルからの有用微生物の スクリーニング
道久 則之; 伊藤 政博; 宮崎明日香; 境貴子; 熊坂尚亮
しんかいシンポジウム, Feb. 2006 - Brachybacterium sp. LB25株の生産するG3アミラーゼの精製と諸性質の解析
道久 則之; 山岸亘
極限微生物学会, Nov. 2005 - Characterization of a maltooligosaccharides-forming amylase from Brachybacterium sp. strain LB25 which improves product selectivity in the presence of organic solvents
道久 則之; 山岸亘
International Symposium on Extremophiles and Their Applications（国際会議）, Nov. 2005 - 有機溶媒耐性シクロデキストリン合成酵素の探索と解析
道久 則之; 桑原宏和; 青野力三
日本農芸化学会, Mar. 2005 - 有機溶媒耐性シクロデキストリン合成酵素の探索
道久 則之; 桑原宏和; 青野力三
極限微生物学会, Nov. 2004 - Screening and application of organic solvent resistant microorganisms
道久 則之
国際会議 International congress for culture collections, Oct. 2004 - Acinetobacter sp. ST-550株由来のフェノールヒドロキシラーゼ遺伝子を導入した大腸菌による有機溶媒存在下におけるインジ後の生産
道久 則之; 豊田晃一; 青野力三
日本農芸化学会, Mar. 2003 - 有機溶媒-培養液の二相系においてインジゴを効率よく生産する微生物の検索
道久 則之; 中野肇; 奥山洋平; 青野力三
日本農芸化学会, Mar. 2001 - 有機溶媒存在下におけるAcinetobacter sp. ST-550株によるインジゴの生産
道久 則之; 中野肇; 奥山洋平; 青野力三
極限微生物学会, Mar. 2001
Research Projects
- ユビキノン生合成系に着目した高度有機溶媒耐性大腸菌の創製
Apr. 2017 - Mar. 2020 - 高度な安定性を有するコレステロール酸化酵素の触媒機構の解明と高活性化
2012 - 2014 - グラム陰性細菌由来新規コレステロール酸化酵素の高度安定化機構の解明
2009 - 2011 - オリゴ糖を生成する有機溶媒耐性アミラーゼの精製と諸性質の解析
2006 - 2006 - 有機溶剤耐性大腸菌を用いた有機溶剤耐性生体触媒の開発
2005 - 2006 - 有機溶媒耐性プロテアーゼおよびアミラーゼの精製と諸性質の解析
2005 - 2005 - 有機溶媒耐性酵素の探索と解析
2004 - 2004 - Improvement of organic solvent tolerance level by directed evolution of AcrAB-TolC efflux pump in Escherichia coli
Grant-in-Aid for Scientific Research (B)
Tokyo Institute of Technology
2000 - 2002 - Organic solvent－resistant enzyme
- Indigo formation in the presence of organic solvent by a microorganism
- Cholesterol conversion in the presence of organic solvent by a microorganism
Industrial Property Rights
