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Toru Mizuki

    Bio-Nano Electronics Research Center　Research Associate
Last Updated :2025/04/19

Researcher Information

URL

Research funding number

  • 80408997

J-Global ID

Profile

  • I have received my phD from Toyo University in 2004. I had researched on enzymes from Extremophiles that live in extreme environments such as high or low temperature, acidity or alkalinity, high pressure and so on. My current research interests include bio-nano fusion research such as increase enzyme activity using a rotational magnetic field and development of new materials using a rare sugar for application in biological field.

Research Areas

  • Life sciences / Tumor diagnostics and therapeutics
  • Life sciences / Bacteriology
  • Nanotechnology/Materials / Nanobioscience
  • Life sciences / Biomaterials

Published Papers

Conference Activities & Talks

MISC

Research Grants & Projects

  • 日本学術振興会：科学研究費助成事業
    Date (from‐to) : 2021/04 -2024/03 
    Author : 中島 義賢; 酒井 政道; 水木 徹
     
    細胞の状態が時々刻々変化していく様子を「電気信号」によって調べることができれば、薬への細胞の感受性、投薬のタイミング、投薬効果の増強、または、副作用の軽減に関する薬学・医学的研究への貢献につながると考え、そのためのデバイス作成を試みている。 これまでは、細胞表面の表面電位の変化により泳動速度が変化することを利用した電気泳動コールター法を提案してきた。今回は、細胞から外部へ放出される情報を分析できることを目的としている。 初年度は、正常細胞と老化細胞の間でのグルコース消費と乳酸産出についてを調べた。今回用いた細胞は、継代回数の少ない正常細胞から継代回数を徐々に増やし、総継代数の異なるいくつかの細胞を作成した。これらの細胞で、ミトコンドリアの活性の低下とそれによる嫌気的解糖により、グルコース消費と乳酸産出が共に徐々に増大していく傾向がみられ、細胞状態の評価を行い、老化細胞の特徴が確認できた。乳酸（イオン）産出時には、溶液中にプロトン(H＋)が放出されている。次の段階として、単一細胞が周辺環境へ放出するプロトン量、または、それにより生じる局所領域のpHの変化を検出することが可能かを試みるためのデバイス作成を開始している。水素ガスをPt電極上で電離させ、その時に生じたプロトンを取り込むことができる材料があり、その候補の中から、細胞培養液の中にあっても期待した材料特性が得られるかについて評価を行っているところである。
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2018/04 -2021/03 
    Author : Nakajima Yoshikata
     
    The purpose of this study was to establish a technology for detecting biological reactions by "electrical signals" in order to turn the lab-on-a-chip into an electronic device. We carried out experiments using samples with different numbers of passages, i.e., normal cells and senescent cells. The Zeta potential was derived from the "electrical signal" obtained when the cells passed through the aperture in a microchannel, and it was confirmed that there was a significant difference between normal and senescent cells. The Zeta potential of the cell surface changed because the amount of glucose uptake increased due to anaerobic glycolysis in the senescent cells, and the amount of lactate ion discharge from the cells increased.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
    Date (from‐to) : 2018/04 -2021/03 
    Author : Mizuki Toru
     
    We aim to develop nanomaterials modified with rare sugars, as a new nanomaterial that can be applied to cancer treatment and medical inspection device, and analyze their functionality. In this study, we synthesized and analyzed reduced graphene oxide functionalized with rare sugars (RSfGO). We obtained a lot of data on RSfGO such as conditions suitable for synthesis, analysis of surface physical characteristics, and biocompatibility/cytotoxicity. The data accumulated in this research is important for the development of new materials using rare sugars in the future and their medical applications.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
    Date (from‐to) : 2017/04 -2020/03 
    Author : Hanajiri Tatsuro
     
    We demonstrated that the ECM can identify whether the present cell state is alive or dead for solutions of IM-9 cells, both before and after dosing using an apoptosis inducer. This approach can be applied to other biological cells and biomaterials, for cases in which their size and/or zeta-potential distributions are influenced by disease. Therefore, the ECM is promising for realization of quick point-of-care diagnostics, such as blood glucose monitoring for diabetic patients and early-stage detection of various diseases in remote healthcare. We also demonstrated that Yttrium hydride (YHx) will be helpful as semiconducting materials for FETs, which would be combined with ECM and graphene oxide(GO) are useful as passivation films of YHx. We also proposed the H-shaped microchannel for suppressing the pressure gradient flow and demonstrated the measurement of the electrophoretic velocity of cells using the proposal microchannel.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)
    Date (from‐to) : 2015/04 -2018/03 
    Author : Nakajima Yoshikata; MIZUKI Toru; UKAI Tomofumi; HANAJIRI Tatsuro
     
    By combining the electrophoresis and conventional Coulter methods, we proposed the electrophoretic Coulter method (ECM) technique that enables the simultaneous analysis of the number, size and Zeta potential (ζ) of individual specimens. We also demonstrated that the ECM can identify whether the present cell state is alive or dead for solutions of human B lymphoblast (IM-9) cells, both before and after dosing of an apoptosis inducer. However, it was difficult to identify cell states in individual cells with the present ECM technique even though size distributions and ζ distributions in IM-9 cells were changed with the time lapse after dosing. We demonstrated the identification of normal cells or dead cells by using cell stain buffer for ECM and their results show difference values in ζ, which are sufficient to separate normal cells or dead cells.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
    Date (from‐to) : 2012/04 -2015/03 
    Author : HANAJIRI TATSURO; MAEKAWA Toru; NAKAJIMA Yoshikata; MIZUKI Toru; HIGASHI Toshiaki
     
    We established some techniques critical for design and fabrication bio-nano fusion systems by using soft lithography. We succeeded in developing a high-performance SPM for soft lithography. This SPM has atomic spatial resolution, and it has advantages for next-generation ultra fine patterning. We proposed that PDMS, grapheme and related materials should be helpful for ultra fine patterning of Si wires to develop novel devices for bio-nano fusion systems. We characterized fundamental properties of the advanced materials, and optimized their coating conditions for soft lithography. Finally, we proposed that SOI(Silicon On Insulator) wafers or SOQ (Silicon On Quartz) wafers should be favorable for platforms of bio-nano fusion systems, and we succeeded in analyzing the behaviors of electric fields in quarts layers of SOI wafers or SOQ wafers by formulating models using conformal transformation technique.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
    Date (from‐to) : 2009 -2011 
    Author : HANAJIRI Tatsuro; YOSHIMORO Tomomi; MIZUKI Toru; KASHIWAGI Kunihiro
     
    By combining the electrophoresis method and the conventional Coulter method, we previously proposed the Electrophoretic Coulter Method(ECM)- a technique that enables simultaneous analysis of the size and ζvalues of individual specimens. In this study, we validate ECM in ζmeasurement of biological cells by a conventional Dynamic Light Scattering(DLS) to apply ECM to the field of clinical medicine. We use sheep's red blood cells(RBCs) as biological cells. The ζof RBC is varied by adsorption of Immunogloblin G(IgG) to RBC. We are able to obtain the difference between ζs of RBCs and those of RBCs-IgG by using ECM. In addition, we demonstrate thatζs obtained using ECM show a good agreement with those obtained by the conventional DLS and our ECM can distinguish biological cells using the difference ofζs. Furthermore, we propose that SOI(Silicon On Insulator) wafers or SOQ(Silicon On Quartz) wafers should be utilized as platforms for integration of ECM systems combined with electric detectors or optical detectors and sorting systems, and we characterize fundamental characteristics of SOI wafers and SOQ wafers in detail.

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