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ウママヘスワリ ラジャゴパランウママヘスワリ ラジャゴパラン

所属・担当
食環境科学部
ライフイノベーション研究所
工業技術研究所
職名助教
メールアドレスumamaheswari[at]toyo.jp ※[at]を@に書き換えて送信して下さい
ホームページURLhttp://kaken.nii.ac.jp/d/r/40270706.ja.html
生年月日
Last Updated :2017/11/21

研究者基本情報

基本情報

    プロフィール:私はインドの南の町Madurai出身で長く日本に住んでいます。日本に学生として来たことがきっかけで日本がもう一つのふる里になりました。私はインドで物理学と光工学の修士課程を修了し、その後、北海道大学で光学の基礎研究で博士号を取得しました。神奈川科学技術アカデミーと理化学研究所では、それぞれ、ナノフォトニクス技術の生体応用と脳科学の分野で視覚野と嗅覚野を対象として光応用計測の研究に従事したため、光の基礎・ナノ光学から脳科学まで非常に広範囲の知識を得ることができました。今までの研究者としての経験と応用言語で学んでいることを活かし、科学技術用英語教育を含む研究・教育に非常に強く関わりたいと思っています。

学位

  • 工学博士, 北海道大学
  • M.Tech (Applied Optics), Indian Institute of Technology, Delhi, India
  • M.Sc. (Physics), American College, Madurai, india
  • M.Ed. (applied Linguistics), Open University, UK

経歴

  •   2006年04月 - 2014年03月, 理化学研究所, 脳統合機能研究チーム, 嘱託研究員
  •   1998年04月 - 2006年03月, 理化学研究所, 脳統合機能研究チーム, 研究員
  •   1993年04月 - 1998年03月, 神奈川科学技術アカデミー, 大津フォトンコントールプロジェークト, 研究員
  •   1992年03月 - 1993年03月, Joensuu university, Dept. of Physics, 研究員

研究活動情報

研究分野

  • 応用物理学・工学基礎, 応用光学・量子光工学
  • 電気電子工学, 計測工学

論文

  • An optical interferometric technique for assessing ozone induced damage and recovery under cumulative exposures for a Japanese rice cultivar., Thilakarathne BL, Rajagopalan UM, Kadono H, Yonekura T, SpringerPlus, 3,   2014年02月
  • Highly sensitive optical interferometric technique reveals stress-dependent instantaneous nanometric growth fluctuations of Chinese chive leaf under heavy metal stress., Muthumali DeSilva KT, Rajagopalan UM, Kadono H, Ecotoxicology and environmental safety, 137, 86 - 93,   2017年03月
  • Swept source optical coherence tomography as a tool for real time visualization and localization of electrodes used in electrophysiological studies of brain in vivo., Watanabe H, Rajagopalan UM, Nakamichi Y, Igarashi KM, Kadono H, Tanifuji M, Biomedical optics express, 2, (11) 3129 - 3134,   2011年11月
  • In vivo layer visualization of rat olfactory bulb by a swept source optical coherence tomography and its confirmation through electrocoagulation and anatomy., Watanabe H, Rajagopalan UM, Nakamichi Y, Igarashi KM, Madjarova VD, Kadono H, Tanifuji M, Biomedical optics express, 2, (8) 2279 - 2287,   2011年08月
  • Using the light scattering component of optical intrinsic signals to visualize in vivo functional structures of neural tissues., Rajagopalan UM, Tsunoda K, Tanifuji M, Methods in molecular biology (Clifton, N.J.), 489, 111 - 132,   2009年
  • Functional optical coherence tomography reveals localized layer-specific activations in cat primary visual cortex in vivo., Rajagopalan UM, Tanifuji M, Optics letters, 32, (17) 2614 - 2616,   2007年09月
  • Localization of activity-dependent changes in blood volume to submillimeter-scale functional domains in cat visual cortex., Fukuda M, Rajagopalan UM, Homma R, Matsumoto M, Nishizaki M, Tanifuji M, Cerebral cortex (New York, N.Y. : 1991), 15, (6) 823 - 833,   2005年06月
  • Novel functional imaging technique from brain surface with optical coherence tomography enabling visualization of depth resolved functional structure in vivo., Maheswari RU, Takaoka H, Kadono H, Homma R, Tanifuji M, Journal of neuroscience methods, 124, 83 - 92,   2003年03月
  • Fabrication of a pencil-shaped fiber probe with a nanometric protrusion from a metal film for near-field optical microscopy., Mononobe S, Maheswari RU, Ohtsu M, Optics express, 1, 229 - 233,   1997年10月
  • Near-field optical imaging of flagellar filaments of salmonella in water with optical feedback control., Naya M, Micheletto R, Mononobe S, Uma Maheswari R, Ohtsu M, Applied optics, 36, 1681 - 1683,   1997年03月
  • Deducing structural variations of the apex of probes used in near-field optical microscopy through simultaneous measurement of shear force and evanescent intensity., Maheswari RU, Mononobe S, Ohtsu M, Applied optics, 35, 6740 - 6743,   1996年12月

MISC

  • Functional optical coherence tomography of rat olfactory bulb with periodic odor stimulation, Hideyuki Watanabe, Hideyuki Watanabe, Uma Maheswari Rajagopalan, Yu Nakamichi, Kei M. Igarashi, Hirofumi Kadono, Manabu Tanifuji, Biomedical Optics Express, 7,   2016年02月10日, © 2016 Optical Society of America. In rodent olfactory bulb (OB), optical intrinsic signal imaging (OISI) is commonly used to investigate functional maps to odorant stimulations. However, in such studies, the spatial resolution in depth direction (z-axis) is lost because of the integration of light from different depths. To solve this problem, we propose functional optical coherence tomography (fOCT) with periodic stimulation and continuous recording. In fOCT experiments of in vivo rat OB, propionic acid and m-cresol were used as odor stimulus presentations. Such a periodic stimulation enabled us to detect the specific odorresponses from highly scattering brain tissue. Swept source OCT operating at a wavelength of 1334 nm and a frequency of 20 kHz, was employed with theoretical depth and lateral resolutions of 6.7 μm and 15.4 μm, respectively. We succeeded in visualizing 2D cross sectional fOCT map across the neural layer structure of OCT in vivo. The detected fOCT signals corresponded to a few glomeruli of the medial and lateral parts of dorsal OB. We also obtained 3D fOCT maps, which upon integration across z-axis agreed well with OISI results. We expect such an approach to open a window for investigating and possibly addressing toward inter/intra-layer connections at high resolutions in the future.
  • Monitoring of growth dynamics of plants under the influence of cadmium using a highly sensitive interferometry technique, Kokge Thilini Kanchana Muthumali De Silva, Kokge Thilini Kanchana Muthumali De Silva, Uma Maheswari Rajagopalan, Hirofumi Kadono, Optical Engineering, 55,   2016年12月01日, © 2016 Society of Photo-Optical Instrumentation Engineers (SPIE).Using statistical interferometry technique (SIT), a highly sensitive interferometry technique developed in our laboratory, we reported about the existence of nanometric intrinsic fluctuations (NIF) in a variety of plants. SIT permits noncontact, noninvasive, and fast detection of plant growth fluctuations in subnanometer scale. We propose the application of NIF to investigate the effect of heavy metal, cadmium, on growth dynamics of Chinese chive (Allium tuberosum). NIFs of leaves were observed for 3 days under four different concentrations of CdCl2: 0, 0.001, 0.01, and 0.1 mM. Results showed significant reduction of NIFs within 4 h for all Cd concentrations, and there was a further decrease with the exposure time of Cd under 0.1 and 0.01 mM. In addition, under 0.001 mM, a significant recovery could be observed after a rapid reduction in the first 4 h. As a comparison, measured antioxidative enzymes increased with increasing Cd concentration. However, no significant increase could be seen within the initial 4 h under a smaller concentration of 0.001 mM as seen for NIFs. The results imply that NIF can be used as an indicator for heavy metal stress on plants as well as it can be more sensitive to detect the influence of smaller Cd amounts on plants at an early stage.
  • High speed and high precision optical interferometric technique to investigate instantaneous growth related changes of plant leaves, THILAKARATHNE Bodhipaksha L.S., RAJAGOPALAN Uma Maheswari, KADONO Hirofumi, YONEKURA Tetsushi, Plant Biotechnol, 31, (3) 195-205 (J-STAGE) - 205,   2014年01月01日, © 2014 The Japanese Society for Plant Cell and Molecular Biology. Instantaneous growth related changes of leaves of Radish, Soybean and rice plants were investigated with a highspeed and ultra-high accuracy optical interferometer. The interferometer, called statistical interferometer, SIT (Statistical Interferometric Technique) is a real-time, non-contact, and robust one and has a subnanometric accuracy and can measure leaf elongation changes every 500 ms. SIT revealed the existence of nanometric fluctuations in the elongation of leaves. Control experiments with dead and mature leaves did indeed establish the existence of null or smaller standard deviations in the fluctuations in contrast to the large deviations obtained for young and healthier leaves. Moreover, the standard deviations of the fluctuations were found to be sensitive to environment of ozone(O3), a secondary atmospheric pollutant. Long term O3exposure with Radish and Soybean leaves led to a decrease in the standard deviations of nanometric fluctuations with increase in O3concentrations which correlated with a decrease of gas exchange measures. Under short term O3exposures for 3 h with two Japanese rice cultivars, cv. Koshihikari and cv. Fusaotome that are known to have varying O3tolerance made the nanometric fluctuations to decrease in comparison to gas exchange measures which showed no variation. The fluctuations could also differentiate the cultivar’s sensitivity to O3with cv. Koshihikari showing a drastic reduction in comparison to cv. Fusaotome. The results suggest that the nanometric fluctuations may possibly relate to the life maintaining physiological state of the plant and thus may act as an indicator of the vitality of the plant.
  • Mapping of Brain Functions at Micron Level with Functional Optical Coherence Tomography-a Correlation Study with Electrophysiological Recordings, RAJAGOPALAN Uma Maheswari, TANIFUJI Manabu, 東洋大学工業技術研究所工業技術, (37) 61 - 72,   2015年02月19日
  • Adopting a SFL-oriented approach for evaluating genre-based academic abstracts of EST undergraduate students in Japan, Rajagopalan Uma Maheswari, Jie Shi, IEEE Conference Proceedings, 2016, (ICAMechS) 510‐515 - 515,   2016年, © 2016 IEEE.Research abstract is a text of foremost importance to students of science and engineering. However, explicit teaching of it tends to be missing in EGP and EGAP curricula of general Japanese English education. This paper reports the evaluation of abstracts written by 3rd-year students in an undergraduate ESP course at a Japanese university of science and engineering. At first, students were taught the required generic moves for a research abstract (RA). Next, students were asked to practice deconstructing abstracts into moves within the classroom as well as with abstracts collected by themselves. Finally, students constructed their own abstracts. Here, in order to construct one's own abstract, each student was asked to select an experiment done towards his or her disciplinary curriculum and use the experimental procedure, results and implications in writing the moves of the abstract. A total of twenty abstracts were analyzed for the presence of the required moves and the sequence of the generic moves. Difficulties in students' abstract writings were evaluated using Systemic Functional Linguistics (SFL) as a framework for the analysis. SFL as a theory of language views language as a resource for making meaning and does linguistic analysis paying attention to different lexico-grammatical structures in constructing meanings and also the context. Students' abstracts were analyzed for different SFL features namely, ideational, interpersonal and textual modes. In particular, the focus was given to the usage of hedges and modality as research abstracts contain modality and hedges. In addition, a clause by clause analysis of students' abstracts revealed common errors such as, singular-plural mismatch, referencing, missing and wrong preposition, improper use of lexis and mistake in verbal group. In the talk, identified errors and the pedagogical ramifications in teaching abstracts will be discussed.
  • Genre-based framework to reach a more succinct and objective decision in peer review of a research article, Uma Maheswari Rajagopalan, Shi Jie, International Conference on Advanced Mechatronic Systems, ICAMechS,   2014年01月01日, © 2014 IEEE. In research publications, peer review is an essential step in process of research article (RA) evaluation for publication by independent scientists. Reviewers are obligated to conduct the review objectively and provide with appropriate criticisms supported by proper evidence and adequate comments in confidence. The comments serve the purpose of informing journal editors about article content and the significance of the results. However, in the absence of a succinct and objective frameworks of reviewing and adequate training, peer reviewers are inclined to give general and, at times, non-systematic comments on the RAs under evaluation. To address this issue, a holistic framework and a specific checklist based on the RA genre and the authentic guideline of a scientific journal is proposed in this paper. The framework is expected to give a holistic structure for writing peer reviews and to especially benefit novel reviewers. The checklist mainly consists of the following genre-based moves: research purpose and/or rational in the appropriate contexts of the research fields, experimental and theoretical explanations, significance of outcome/results, and other relevant discussions and perspectives.
  • Teaching poster by using genre approach to science and technology students of A Japanese university, Uma Maheswari Rajagopalan, Uma Maheswari Rajagopalan, Jie Shi, International Conference on Advanced Mechatronic Systems, ICAMechS, 2015-October,   2015年10月01日, © 2015 IEEE. Science and technology students require the specialized skill of making poster and the explicit teaching of which is lacking in the curricula of general Japanese English tertiary education. This paper considers posters as an expanded form of abstracts and reports teaching of posters to 20 third-year students of an undergraduate ESP course at a Japanese university of science and engineering. First, students were taught to write an abstract using genre theory which required teaching of the generic moves of an abstract of a research article (RA). Teaching of the abstract involved the explicit teaching of the basic moves of background, purpose, methods, results and summary that are needed to construct RA abstract. Following that, students deconstructed abstracts taken from authentic research articles selected by both the teacher and the student themselves into moves. Having understood the moves, a democratic choice of several topics from the experiments done as part of the subject curriculum was chosen for constructing abstracts. The abstract drafts were peer evaluated with checklists, followed by teacher's comments based on which the students submitted a final draft. Next, with the final draft, the students expanded the abstract to construct a poster. This task involved writing the contents of each of the moves in the abstract to detailed content expanding each of the sections, Introduction, Methods, Results and Summary. Contents of all the sections except for the Results were prepared in groups of three or four with each member being responsible for each section. Although group members constructed the contents through discussion, poster making and presentation were done individually. Though there were some overlaps in the contents, the current project was found to empower the students with the required poster making and presentation skills which was also shown from the post survey.
  • Functional optical coherence tomography (fOCT) biospeckle imaging to investigate response of plant leaves to ultra-short term exposure of Ozone, L. K T Srimal, L. K T Srimal, U. M. Rajagopalan, H. Kadono, Journal of Physics: Conference Series, 605,   2015年01月01日, © Published under licence by IOP Publishing Ltd. In this study, response of leaves of Chinese chives (Allium tuberosum) to ozone stress was investigated using functional optical coherence tomography (fOCT) based on biospeckle. The biospeckles arising out of dynamic motion of organelles can reflect the biological activities of plant. The fOCT biospeckle image was obtained by calculating the standard deviation (SD) of the fOCT temporal signal (biospeckle signal) at each and every point from the successively acquired OCT images. Plant leaves were subjected to treatment under different concentrations of O3, and imaging data were acquired from back and front surfaces of the leaves. The internal cell structure within the Chinese chives leaves could be clearly visualized in the functional OCT biospeckle image, which was not clearly visible in conventional OCT cross-sectional image. The SDs were found to be increasing significantly, especially in the surface layers of both front and back sides of the leaf with ozone exposure. Thus, the fOCT based on biospeckle is found to be suitable for fast, non-destructive monitoring environmental stresses on plants, which can potentially lead to significant time saving, for which conventional techniques require a few days to a few weeks time.
  • 機能光断像画像j去によるミクロンレーベル脳機能マッピングー電気生理学との相関研究, ラジャゴパランウママヘスワリ, 谷藤 学, 工業技術 : 東洋大学工業技術研究所報告, (37) 61 - 72,   2015年
  • Optical coherence tomography biospeckle imaging for fast monitoring varying surface responses of a plant leaf under ozone stress, L. K T Srimal, L. K T Srimal, H. Kadono, U. M. Rajagopalan, Proceedings of SPIE - The International Society for Optical Engineering, 8881,   2013年08月01日, In this study, Optical Coherence Tomography (OCT) was used to investigate the effect of short term ozone exposure on both front and back surfaces of the leaves of Allium tuberosum plant. Plant leaves were subjected to treat with 240ppb level of ozone, and imaging data were acquired from back and front surfaces of the leaves. Variations of the biological activations were monitored based on the biospeckles. Standard deviations (SD) of OCT temporal signal at each point were calculated and used to visualize the effect of ozone exposure. Leaf back surface showed higher SDs in biospeckle OCT signal in comparison to these of the front surface. These observations prove that the short term ozone stress on plant can be investigated successively with biospeckle OCT imaging technique. © 2013 SPIE.
  • J022021 フーリエイメージングに基づく機能的0CTを用いた脳機能の3次元マイクロ計測([J02202]診療技術と臨床バイオメカニクス(2)), 中道 友, KALATSKY Valery A., 渡邊 秀行, RAJAGOPALAN Uma Maheswari, 谷藤 学, 年次大会 : Mechanical Engineering Congress, Japan, 2011,   2011年09月11日, Functional optical coherence tomography (functional OCT, fOCT) has been developed as 3-dimensional and micro-scale visualizing technique of brain function. Functional signals are obtained by the changes of OCT signal intensities before and after stimulation. However, it is difficult to provide the brain function due to physiological noises, e.g. heartbeat and respiration, and mechanical artifacts, e.g. intensity fluctuation of light source. In this study, a new fOCT paradigm is proposed by employing Fourier imaging technique, which uses continuous and periodic stimulation and extracts the frequency component at the stimulation to remove periodic noises by Fourier analysis. Proposed technique is applied to cat primary visual cortex in vivo and investigated, comparing with conventional brain imaging, i.e. optical intrinsic signal imaging. As the results, 3-dimensional brain functional map was obtained by fOCT and has high correlation with optical intrinsic signal imaging (R=0.79). Therefore, Fourier imaging based fOCT is promising for 3-dimensional and micro-scale imaging of brain function.
  • 機能的光コヒーレンストモグラフィーによる脳機能計測 (視覚光学と脳機能計測), Rajagopalan Uma Maheswari, 谷藤 学, 光学, 33, (2) 96 - 102,図巻頭1p,   2004年02月
  • Functional optical coherence tomography to reveal functional architecture of cat visual cortex in vivo, Uma Maheswari Rajagopalan, Ryota Homma, Hirofumi Kadono, Manabu Tanifuji, Proceedings of SPIE - The International Society for Optical Engineering, 5140,   2003年12月01日, Optical intrinsic signal imaging (OISI) provides the surface activation map of brain and has provided many insights. In this study, we show that the optical coherence tomography (OCT) can provide depth resolved functional map of cat visual cortex. Activation profile obtained by integrating OCT signal across depth correlates well with that determined by the OISI. Functional OCT (fOCT) promises to be a valid technique for revealing unexplored organization inside the brain at a micro system level.
  • Functional imaging of cat primary visual cortex with optical coherence tomography, Uma Maheswari Rajagopalan, Hideyuki Takaoka, Ryota Homma, Hirofumi Kadono, Manabu Tanifuji, Proceedings of SPIE - The International Society for Optical Engineering, 4619,   2002年01月01日, We report the application of Optical coherence tomography (OCT) for visualizing a one dimensional depth resolved functional structure of cat brain in vivo. The OCT system is based on the known fact that neural activation induces structural changes such as capillary dilation and cellular swelling. Detecting these changes as an amplitude change of the scattered light, an OCT signal reflecting neural activity i.e., fOCT (functional OCT) could be obtained. Experiments have been done to obtain a depth resolved stimulus-specific profile of activation in cat visual cortex. Our results in one dimension indicate that indeed an orientation dependent functional signal could be obtained. Further, we show that this depth resolved fOCT signal is well correlated with the stimulus dependent column determined by OISI. Based on the results, the smallest functional unit in depth, resolved by the proposed system is around 40 μm. We are extending our system to perform two dimensional functional imaging.
  • 低コヒーレンス干渉法によるネコの第一次視覚野の深さ分解機能計測, UmaMaheswari Rajagopalan, 高岡 秀行, 本間 良太, 門野 博史, 谷藤 学, 生物物理, 40, (Suppl.1) S56 - S56,   2000年08月
  • Nanometer Level Resolving Near Field Optical Microscope under Optical Feedback in the Observation of a Single-String Deoxyribo Nucleic Acid, Rajagopalan Uma Maheswari, Mononobe Shuji, Yoshida Kenji, YOSHIMOTO Mamoru, OHTSU Motoichi, Japanese journal of applied physics. Pt. 1, Regular papers & short notes, 38, (12) 6713 - 6720,   1999年12月15日, We present the observation results of a single string deoxyribo nucleic acid (DNA) obtained by a collection mode near field optical microscope (NOM) operated under constant distance mode with the optical near field intensity as the feedback signal. The observed width of the narrowest string is around 4 nm. To our knowledge, this is not only the successful optical observation of a single string DNA by purely optical means but also under such high resolving capability. This high resolution is mainly attributed to the special care taken during the preparation of the sample and of the probe to avoid unwanted scattered light. The probe efficiently picks up the high spatial frequency component of the scattered near field and simultaneously rejects out effectively the lower spatial frequency components.
  • 1G1730 低コヒーレンス干渉法によるネコの第1次視覚野の深さ分解機能計測, Maheswari Rajagopalan Uma, 高岡 秀行, 本間 良太, 門野 博史, 谷藤 学, 生物物理, 40,   2000年08月05日
  • Functional Optical Coherence Tomography for imaging brain functions, RAJAGOPALAN Uma Maheswari, WATANABE Hideyuki, IGARASHI Kei, TANIFUJI Manabu, 応用物理学会学術講演会講演予稿集, 69th,   2008年09月02日
  • Functional optical coherence tomography reveals localized layer-specific activations in cat primary visual cortex in vivo, RAJAGOPALAN Uma Maheswari, TANIFUJI Manabu, Opt Lett, 32,   2007年09月01日
  • Functional Optical Coherence Tomography for imaging brain functions, RAJAGOPALAN Uma Maheswari, 応用物理学関係連合講演会講演予稿集, 52nd,   2005年03月29日
  • 機能的光コヒーレンストモグラフィーによる脳機能計測, ラジャゴパラン ウママヘスワリ, 谷藤 学, 光学, 33, (2) 96 - 102,   2004年02月10日
  • 光を使った脳機能計測 : 脳活動による神経組織の光特性変化のイメージング, ラジャゴパラン ウママヘスワリ, 谷藤 学, 應用物理, 72, (6) 711 - 715,   2003年06月10日
  • 機能的OCTによる脳機能イメージング, ラジャゴパラン ウママヘスワリ, 谷藤 学, レーザー研究, 30, (11) 648 - 655,   2002年11月15日, We review the application of Optical coherence tomography (OCT) for visualizing a depth resolved functional structure of cat brain in vivo. The OCT system is based on the known fact that neural activation induces structural changes such as capillary dilation and cellular swelling. Detecting these changes as an amplitude change of the scattered light, an OCT signal reflecting neural activity i.e., fOCT (functional OCT) could be obtained. Experiments have been done to obtain a depth resolved stimulus-specific profile of activation in cat visual cortex.
  • 2P215ネコの第1視覚野における機能的OCT信号と方位選択性マップの比較, Uma Maheswari Rajagopalan, 門野 博史, 谷藤 学, 生物物理, 41,   2001年09月10日
  • Nanometer level resolving near field optical microscope under optical feedback in the observation of a single-string deoxyribo nucleic acid, Uma Maheswari Rajagopalan, Uma Maheswari Rajagopalan, Shuji Mononobe, Kenji Yoshida, Mamoru Yoshimoto, Motoichi Ohtsu, Motoichi Ohtsu, Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers, 38, (12) 6713 - 6720,   1999年12月01日, We present the observation results of a single string deoxyribo nucleic acid (DNA) obtained by a collection mode near field optical microscope (NOM) operated under constant distance mode with the optical near field intensity as the feedback signal. The observed width of the narrowest string is around 4 nm. To our knowledge, this is not only the successful optical observation of a single string DNA by purely optical means but also under such high resolving capability. This high resolution is mainly attributed to the special care taken during the preparation of the sample and of the probe to avoid unwanted scattered light. The probe efficiently picks up the high spatial frequency component of the scattered near field and simultaneously rejects out effectively the lower spatial frequency components. © 1999 Publication Board, Japanese Journal of Applied Physics.
  • 2P214内因性信号のイメージングにより調べた神経活動依存的な局所血液量変化の空間分解能, 福田 光洋, Uma Maheswari Rajagopalan, 本間 良太, 松元 まどか, 西崎 誠, 谷藤 学, 生物物理, 41,   2001年09月10日
  • Observation of DNA fixed on a sapphire substrate by a collection mode near-field optical microscope, Uma Maheswari Rajagopalan, Shuji Mononobe, Kenji Yoshida, Mamoru Yoshimoto, Motoichi Ohtsu, Proceedings of SPIE - The International Society for Optical Engineering, 3729,   1999年01月01日, We present the observation results of a single string DNA (deoxy ribo nucleic acid) obtained by collection mode near-field optical microscope (NOM) operated under constant distance mode with the optical near field intensity as the feedback signal. The observed width of the narrowest string is 4 nm. To our knowledge, this is not only the successful optical observation of a single string DNA by purely optical means but also under such high resolving capability. These are attributed to the special care taken during the sample preparation to avoid unwanted scattered light and to the probe which were specially designed to enhance the local near field interaction. All these factors make the probe picks up efficiently the high spatial frequency components of the scattered near field at the same time rejecting effectively the lower spatial frequency components.
  • Observation of Subcellular Structures of Neurons by an Illumination Mode Near-Field Optical Microscope under an Optical Feedback Control, UMA MAHESWARI Rajagopalan, MONONOBE Shuji, TATSUMI Hitoshi, KATAYAMA Yoshifumi, OHTSU Motoichi, Optical review, 3, (6) 463 - 467,   1996年12月01日
  • Observation of Subcellular Structures of Neurons by an Illumination Mode Near-Field Optical Microscope under an Optical Feedback Control*, MAHESWARI Rajagopalan UMA, MONONOBE Shuji, TATSUMI Hitoshi, KATAYAMA Yoshifumi, OHTSU Motoichi, Optical Review, 3, (6) 463 - 467,   1996年, We propose an illumination mode near-field optical microscope operated under an optical feedback for imaging biological specimens in their natural environment. For feedback control, rapidly varying evanescent signal has been generated over the sample surface. It has been found that evanescent signal can be profitably used as a control signal by utilizing its sample feature dependent discrimination sensitivity. Neurons have been successfully observed both in air and in liquid with a resolution well beyond the diffraction limit.

書籍等出版物

  • Dynamic Brain Imaging: Multi-Modal Methods and In Vivo Applications (Methods in Molecular Biology)
    Humana Press  2014年11月03日
  • Near-field Nano/Atom Optics and Technology
    Springer  2012年10月23日
  • Nano-Optics (Springer Series in Optical Sciences)
    Springer  2011年09月22日