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Ritsuko Kaneko

Faculty
Department of Life Sciences
Research Institute of Industrial Technology
Research Center for Biomedical Engineering
Course of Life Sciences
PositionProfessor
Mail
HomepageURL
Birthday
Last Updated :2019/10/04

Researcher Profile and Settings

Education

  •  - 1982 , The University of Tokyo, Graduate School, Division of Science
  •  - 1980 , The University of Tokyo, Faculty of Science

Degree

  • Ph.D, Yamagata University
  • Master of Science, The University of Tokyo

Academic & Professional Experience

  •   1992  - 1997 , St.Marianna University School of Medicine, lecturer
  •   1997 , St.Marianna University School of Medicine, Assistant Professor
  •   1990  - 1992 , St.Marianna University School of Medicine, Research Assistant
  •   1988  - 1989 , Alberta University , Post doctoral Research Fellow
  •   1983  - 1988 , Yamagata University, Research Assistant

Research Activities

Research Areas

  • Neuroscience, Nerve anatomy/Neuropathology
  • Cerebral Neuroscience, Neuroscience in general
  • Basic biology, Animal physiology/Animal behavior

Research Interests

    vessel models, microchip, Developmental Biology

Published Papers

  • Crmp4-KO mice as an animal model for investigating certain phenotypes of autism spectrum disorders, Ohtani-Kaneko Ritsuko, International Journal of Molecular Sciences, 20, 2485 - 2500, 05 , Refereed, Invited
  • Androgen induced cellular proliferation, neurogenesis, and generation of GnRH3 neurons in the brain of mature female Mozambique tilapia., Y. Narita, A. Tsutiya, Y. Nakano, M. Ashitomi, K. Sato, K. Hosono,T. Kaneko, R-D. Chen, J-R. Lee, Y-C. Tseng, P-P Hwang & R. Ohtani-Kaneko, Scientific Reports, 8, (1) , 11 , Refereed
  • Administration of hydrogen-rich water prevents vascular aging of the aorta in LDL receptor-deficient mice., Masumi Iketani , Kanako Sekimoto , tsutomu igarashi , Mayumi Takahashi , Masaki Komatsu , Iwao Sakane , Hiroshi Takahashi , Hideo Kawaguchi , Ritsuko Ohtani-Kaneko & Ikuroh Ohsawa, Scientific Reports, 8, (1) , 11 , Refereed
  • Human CRMP4 mutation and disrupted Crmp4 expression in mice are associated with ASD characteristics and sexual dimorphism., Tsutiya A, Nakano Y, Hansen-Kiss E, Kelly B, Nishihara M, Goshima Y, Corsmeier D, White P, Herman GE, Ohtani-Kaneko R., Sci Rep., 17, (1) 16812 - (14), 12 , Refereed
  • Characterisation of human induced pluripotent stem cell-derived endothelial cells under shear stress using an easy-to-use microfluidic cell culture system., Ohtani-Kaneko R, Sato K, Tsutiya A, Nakagawa Y, Hashizume K, Tazawa H, Biomed Microdevices., 19, (4) 91 - 101, 04 , Refereed
  • Deletion of collapsin response mediator protein 4 results in abnormal layer thickness and elongation of mitral cell apical dendrites in the neonatal olfactory bulb., Tsutiya A, Watanabe H, Nakano Y, Nishihara M, Goshima Y, Ohtani-Kaneko R., Journal of Anatomy, 228, (5) 792 - 804, 05 , Refereed
  • An Easy-to-Use Polystyrene Microchip-based Cell Culture System., Tazawa H, Sunaoshi S, Tokeshi M, Kitamori T, Ohtani-Kaneko R., Anal Sci., 32, (3) 349 - 353, 04 , Refereed
  • Mouse pups lacking collapsin response mediator protein 4 manifest impaired olfactory function and hyperactivity in the olfactory bulb., Tsutiya A, Nishihara M, Goshima Y, Ohtani-Kaneko R., Eur J Neurosci., 42, (6) 2335 - 2345, 08 , Refereed
  • A microfluidic cell culture system for monitoring of sequential changes in endothelial cells after heat stress., Tazawa H, Sato K, Tsutiya A, Tokeshi M, Ohtani-Kaneko R., Thromb Res, 136, (2) 328 - 334, Refereed
  • Dual cell protective mechanisms activated by differing levels of oxidative stress in HT22 murine hippocampal cells., Sato K, Yamanaka Y, Ishii M, Ishibashi K, Ogura Y, Ohtani-Kaneko R, Nishihara M, Nedachi T., Biosci Biotechnol Biochem., July 25, 1 - 9, 07 , Refereed
  • Collapsin response mediator protein 4 affects the number of tyrosine hydroxylase-immunoreactive neurons in the sexually dimorphic nucleus in female mice., T.Iwakura M.Sakoh, A.Tsutiya, N.Yamashita, A.Ohtani, M.Tsuda, S.Ogawa, S.Tsukahara, M.Nishihara, T.Shiga, Y.Goshima, T.Kato, R.Ohtani-Kaneko., Developmental Neurobiology, 73, (2) 502 - 517, 04 , Refereed
  • Postnatal alteration of collapsin response mediator protein 4 mRNA expression in the mouse brain., A. Tsutiya, R. Ohtani-Kaneko, J. Anatomy, 221, 341 - 351, 07 , Refereed
  • 17β-estradiol prevents reduction of retinal phosphorylated 14-3-3 zeta protein levels following a neurotoxic insult., N. Koseki, Y.Kitaoka, Y.Munamasa, K. Kojima, S. Ueno, Ohtani-Kaneko R., 1433, 145 - 152, 01 , Refereed
  • Sexual dimorphism of gonadotropin-releasing hormone type-III (GnRH3) neurons and hormonal sex reversal of male reproductive behavior in Mozambique tilapia, Asami Kuramochi, Atsuhiro Tsutiya, Toyoji Kaneko, Ritsuko Ohtani-Kaneko, 28, 733 - 739, 10 , Refereed
  • Sexually dimorphic effects of estrogen on spines in cultures of accessory olfactory bulb, Yue Wu, Keiko Moriya-Ito, Takashi Iwakura, Atsuhiro Tsutiya, Masumi Ichikawa, Ritsuko Ohtani-Kaneko, 500, 77 - 81, 08 , Refereed
  • Proteomic surveillance of autoantigens in patients with Behcet’s disease by a proteomic approach., Ooka S., Nakano H., Matsuda T., Okamoto K, Suematsu N, Kurokawa S.,, Masuko K. Ozaki S., Kato T., Microbiology & Immunology, 54, 354 - 361, 07 , Refereed
  • In vitro effects of bisphenol A on developing hypothalamic neurons., Iwakura T., Iwafuchi M., Muraoka D., Yokosuka M., Shiga T., Watanabe ch., Ohrnai-Kaneko R., Toxicology, 272, (41642) 52 - 58, 06 , Refereed
  • Effects of estrogen on synapsin I distribution in developing hypothalamic neurons., Ohtani-Kaneko R., Iwafuchi, M. Iwakura1 T., Muraoka D., Yokosuka M.、Shiga T., Watanabe C. , Neurosci. Res., 66, 180 - 188, 02
  • Involvement of post-translational modification of neuronal plasticity-related proteins in hyperalgesia revealed by a proteomic analysis. Proteomics, Fujisawa H, Ohtani-Kaneko R, Naiki M, Okada T, Masuko K, Yudoh K, Suematsu N, Okamoto K, Nishioka K, Kato T., Proteomics., 8, (8) 1706 - 1719, 04 , Refereed
  • Suppressive effects of cadmium on neurons and affected proteins in cultured developing cortical cells. , Ohtani-Kaneko R, Tazawa H, Yokosuka M, Yoshida M, Satoh M, Watanabe C., Toxicology, 253, (41642) 110 - 116, 04 , Refereed
  • Effects of unoprostone on phosphorylated extracellular signal-regulated kinase expression in endothelin-1-induced retinal and optic nerve damage., Munemasa Y, Kitaoka Y, Hayashi Y, Takeda H, Fujino H, Hirata K, Ueno S.(St.Marianna University School of Medicine), Vis Neurosci., 25, (2) 197 - 208, 03 , Refereed
  • Estrogen and environmental estrogenic chemicals exert developmental effects on rat hypothalamic neurons and glias., Yokosuka M, Ohtani-Kaneko R, Yamashita K, Muraoka D, Kuroda Y, Watanabe C., Toxicolgy In Vitro., 22, 1 - 9, 01 , Refereed
  • Dorsal root ganglion regulates the transient ERK activation in the dorsal horn of the spinal cord during development., Kato T, Ohtani-Kaneko R, Shiga T., Neurosci Research., 58, 402 - 405, 01 , Refereed
  • Neuroprotective effect of 17β-estradiol against NMDA-induced retinal neurotoxicity via p-ERK induction, Hayashi Y, Kitaoka Y, Munemasa Y, Ohtani-Kaneko R, Kikusui T, Uematsu A, Takeda H, Hirata K, Mori Y, Ueno S., Journal of Neuroscience Research, 85, 386 - 494, 01 , Refereed
  • Pro-apoptotic role of c-Jun in NMDA-induced neurotoxicity in the rat retina., Munemasa Y, Ohtani-Kaneko R, Kitaoka Y, Kumai T, Kitaoka Y, Hayashi Y, Watanabe M, Takeda H, Hirata K, Ueno S., J Neurosci Res, 83, 907 - 918, 04 , Refereed
  • Mechanisms underlying estrogen-induced sexual differentiation in the hypothalamus., Ritsuko Ohtani-Kaneko, Histol Histopathol, 21, 317 - 324, 03 , Refereed
  • Liver-type Fatty Acid-binding protein attenuates renal injury induced by unilateral ureteral obstruction., Kamijo-Ikemori A, Sugaya T, Obama A, Hiroi J, Miura H, Watanabe M, Kumai T, Ohtani-Kaneko R, Hirata K, Kimura K., American Journal of Pathology, 169, 1107 - 1117, 01 , Refereed
  • Chloride turnover and ion-transporting activities of yolk-sac preparations (yolk balls) separated from Mozambique tilapia embryos and incubated in freshwater and seawater. , Hiroi J, Miyazaki H, Katoh F, Ohtani-Kaneko R, Kaneko T., J Exp Biol, 208, 3851 - 3858, 10 , Refereed
  • Differential expression of p160 steroid receptor coactivators in the rat testis and epididymis., Igarashi-Migitaka J, Takeshita A, Koibuchi N, Yamada S, Ohtani-Kaneko R, Hirata K., Eur. J. Endocrinol, 153, 1 - 11, 10 , Refereed
  • Functional classification of mitochondrion-rich cells in euryhaline Mozambique tilapia (Oreochromis mossambicus) embryos, by means of triple immunofluorescence staining for Na+/K+-ATPase, Na+/K+/2Cl- cotransporter and CFTR anion channel., Hiroi J, McCormick SD, Ohtani-Kaneko R, Kaneko T., J. Exp. Biol, 208, 2023 - 2036, 06 , Refereed
  • Contribution of mitogen-activated protein kinases to NMDA-induced neurotoxicity in the rat., Munemasa Y, Ohtani-Kaneko R, Kitaoka Y, Kuribayashi K, Isenoumi K, Kogo J, Yamashita K, Kumai T, Kobayashi S, Hirata K, Ueno S., Brain Res, 1044, 227 - 240, 04 , Refereed
  • Developmental Regulation of Activated ERK Expression in the Spinal Cord and Dorsal Root Ganglion of the Chick Embryo., Kato T, Ohtani-Kaneko R, Ono K, Okado N, Shiga T., Neurosci. Res, 52, 11 - 19, 01 , Refereed
  • Down-regulation of BRCA1-BARD1 ubiquitin ligase by CDK2., Hayami R, Sato K, Wu W, Nishikawa T, Hiroi J, Ohtani-Kaneko R, Fukuda M, Ohta T., Cancer Res, 65, 6 - 10, 01 , Refereed
  • Urinary excretion of fatty acid-binding protein reflects stress overload on the proximal tubules, Kamijo-Ikemori A, Sugaya T, Obama A, Hiroi J, Miura H, Watanabe M, Kumai T, Ohtani-Kaneko R, Hirata K, Kimura K., American Journal of Pathology, 165, (1243-1255) 1243 - 1255, 10 , Refereed
  • Structure and developmental expression of hatching enzyme genes of the Japanese eel Anguilla japonica : an aspect ofthe evolution of fish hatching enzyme gene., Hiroi J, Maruyama K, Kawazu K, Kaneko T, Ohtani-Kaneko R, Yasumasu S., Development genes and evolution, 214, (176-184) 176 - 184, 03 , Refereed
  • Circadian rhythm of melatonin release from the photoreceptive pineal organ of a teleost, Ayu (plecoglossus altivelis )in flow.. Thorough culture., Iigo M, Fujimoto Y, Gunji-Suzuki M, Yokosuka M, Hara M, Ohtani-Kaneko R, Tabata M, Aida K, Hirata K., Journal of Neuroendocrinology, 16, 45 - 51, 01 , Refereed
  • Melatonin, a pineal secretory product with antioxidant properties, protects against cisplatin-induced nephrotoxicity in rats., Hara M, Yoshida M, Nishijima H, Yokosuka M, Iigo M, Ohtani-Kaneko R, Shimada A, Hasegawa T, Akama Y, Hirata K., Journal of Pineal Research, 30, 129 - 138, 04 , Refereed
  • Accumulation of phosphorylated neurofilaments and increase in apoptotic specific protein (ASP) and phosphorylated c-Jun induced by proteasome inhibitors., Masaki R, Saito T, Yamada K, Ohtani-Kaneko R., Journal of Neuroscience Research, 62, (1) 75 - 83, 10 , Refereed
  • Developmental changes in the localization of activated C-jun N-terminal kinase (JNK/SAPK) in the chick spinal cord., Ohtani-Kaneko R, Shiga T, Yamashita K, Masaki R, Hirata K., Journal of Comparative Neurology, 426, (4) 622 - 631, 10 , Refereed
  • Female-soiled bedding induced Fos immunoreactivity in the ventral part of the premammillary nucleus (PMv) of the male mouse., Yokosuka M, Matsuoka M, Ohtani-Kaneko R, Iigo M, Hara M, Hirata K, Ichikawa M., Physiology & Behavior, 68, (41641) 257 - 261, 12 , Refereed
  • Proteasome inhibitors which induce neurite outgrowth from PC12h cells cause different subcellular accumulations of multi-ubiquitin chains., Ohtani-Kaneko R, Takada K, Iigo M, Hara M, Yokosawa H, Kawashima S, Ohkawa K, Hirata K., Neurochemical Research, 23, (11) 1435 - 1443, 11 , Refereed
  • Ocular melatonin rhythms in the Goldfish, Carassius auratus., Iigo M, Sánchez-Vázquez FJ, Hara M, Ohtani-Kaneko R, Hirata K, Shinohara H, Tabata M, Aida K., Journal of Biological Phythms, 12, (1) 182 - 192, 10 , Refereed
  • Photic and circadian regulations of melatonin rhythms in fishes. , Iigo M, Hara M, Ohtani-Kaneko R, Hirata K, Tabata M, Aida K., Biological Signals, 6, (225) 232 - 232, 07 , Refereed
  • Melatonin binding sites in the Goldfish Retina. , Iigo M, Ohtani-Kaneko R, Hara M, Hirata K, Aida K., Zoological Science, 14, 601 - 607, 04 , Refereed
  • Administration of melatonin and related indoles prevents exercise-induced cellular oxidative changes in rats. , Hara M, Iigo M, Ohtani-Kaneko R, Nakamura N, Suzuki T, Reiter RJ, Hirata K., Biological Signals, 6, (2) 90 - 100, 03 , Refereed
  • Regulation by guanine nucleotides and cations of melatonin binding sites in the goldfish brain. , Iigo M, Ohtani-Kaneko R, Hara M, Hattori A, Takahashi H, Tabata M, Suzuki T, Aida K., Biological Signals, 6, (1) 29 - 39, 01 , Refereed
  • Characterization, Guanosine 5'-O-(3-thiotriphosphate) modulation, daily variation, and localization of melatonin-binding sites in the catfish (Silurus a sotus) Brain (共著), Iigo M, Hattori A, Ohtani-Kaneko R., Aida K, General and Comparative Endocrinology, 108, 45 - 55, Refereed
  • Nerve Growth factor (NGF) induces increase in multi-ubiquitin chains and concomitant decrease in free ubiquitin in nuclei of PC12h. , Ohtani-Kaneko R, Asahara M, Takada K, Kanda T, Iigo M, Hara M, Yokosawa H, Ohkawa K, Hirata K., Neuroscience Research, 26, (4) 349 - 355, 12 , Refereed
  • Effects of pinealectomy and constant light exposure on day-night changes of melatonin binding sites in the goldfish brain, Iigo M, Furukawa K, Hattori A, Hara M, Ohtani-Kaneko R, Suzuki T, Tabata M, Aida K., Neurosclence Letters, 197, (1) 61 - 64, 09 , Refereed
  • Identification of melatonin in plants and its effects on plasma melatonin levels and binding to melatonin receptors in vertebrates, Hattori A, Migitaka H, Iigo M, Itoh M, Yamamoto K, Ohtani-Kaneko R, Hara M, Suzuki T, Reiter RJ., Biochemistry and molecular biology international, 35, (3) 627 - 634, 03 , Refereed
  • Characteristics, day-night changes, subcellular distribution and localization of melatonin binding sites in the goldfish brain, Iigo M, Kobayashi M, Ohtani-Kaneko R, Hara M, Hattori A, Suzuki T, Aida K., Brain Research, 644, (2) 213 - 220, 05 , Refereed
  • Localization of calcitonin gene-related peptide in the small intestine of various vertebrate species. , Ohtani R, Kaneko T, Kline LW, Labedz T, Tang Y, Pang PK., Cell and Tissue Research, 258, (1) 35 - 42, 10 , Refereed
  • A novel cell type in the parathyroid glands of spontaneously hypertensive rats. , Kaneko T, Ohtani R, Lewanczuk RZ, Pang KTP., Americal Journal of Hypertension, 2, (7) 549 - 552, 07 , Refereed
  • The development of corticospinal tract (CST) neurons. , R.Ohtani, T Shirai, Proceeding of 6th Meeting of Singer Symposium., 6, 473 - 483, 10 , Refereed, Invited
  • Reduction of lipofuscin by centrophenoxine and chlorpromazine in the neurons of rat cerebral hemisphere in primary culture. , Ohtani R, Kawashima S., Experimental Gerontology, 18, 105 - 112, 04 , Refereed

Misc

  • A rotary slicer for brain research, R.Ohtani, H. Kato, J Yamagata School of Med,   1986 , 査読有り
  • The effects of estradiol 17β on the nerite elongation, R. Ohtani, S. Kawashima, Annotationes Zoological Japoneses, 56,   1983 , 査読有り

Research Grants & Projects

  • Mechanisms underlying sexual dimorphism of the brain, Ministry of Education, Culture, Sports, Science and Technology, Grants-in-Aid for Scientific Research(特定領域研究)
  • The effect of steroid hormones on synaptogenesis and its mechanisms, Ministry of Education, Culture, Sports, Science and Technology, Grants-in-Aid for Scientific Research(基盤研究(C)), It has become increasingly clear that estrogens, one of steroids hormones, play an important role in learning and memory across the lifespan. Estrogens also play critical roles in the sexual differentiation of the developing rat hypothalamus to form gender-specific neural circuit during about one perinatal week, critically sensitive period to estrogen. The purpose of this research was to investigate the effect of estrogens on neurons, especially on synaptogenesis during development. We performed this research by using cultured hypothalamic and cortical cells and observed estradiol-17 beta (E2)-mediated changes in synaptogenesis. In some studies, we also used endocrine disruptors with estrogenic activities including bisphenol-A, nonylphenol and cadmium (Cd). Treatment of E2 as well as membrane impermeable E2 conjugated to BSA (E2-BSA) for a week significantly increased the punctual area of synapsin I. The increase in punctuate area of synapsin I induced by E2 or E2-BSA was not inhibited by estrogen receptor antagonist, ICI 182,780. Subcellular distribution pattern of synapsin I, diffusely dispersing or punctually clustering, is controlled by multiple protein kinases. Immunoblots showed that E2 suppressed phosphorylation of synapsin I at sites 1, 2 and 3. These sites are known to be phosphorylated mainly by cAMP-dependent protein kinase (PKA) and Ca2+-calmodulin-dependent protein kinase (CaMK) I and II. On the other hand, E2 did not affect phosphorylation at sites 4/5 and 6 which are phosphorylated mainly by mitogen activated protein kinase (MAPK) . These studies and other inhibitor studies suggested that E2-mediated changes of synapsin I am caused through the non- genomic signaling.
  • Ubiquitin and ubiqutinated proteins in PC12 cells induced by NGF, Ministry of Education, Culture, Sports, Science and Technology, Grants-in-Aid for Scientific Research(一般研究(C), 基盤研究(C)), Ritsuko OHTANI, Changes in ubiquitin-immunoreactivity after nerve growth factor (NGF) treatment were investigated in PC12h cells. Ubiquitin-immunoreactivity was increased in the nucleus of NGF-treated cells. The quantitiy analysis revealed that, after 7days of NGF treatment, almost 20% of cells had ubiquitin-immunoeractive nuclei and the frequency was increased thereafter. Levels of free ubiquitin and multi-ubiquitin chains were measured by radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA), respectively. Measurements were carried out for four subcellular fractions : urea-and water-soluble extracts of nuclei and cytoplasm. Decrease in free ubiquitin was observed in water-soluble cytoplasmic extracts of NGF-treated cells, though increase in multi-ubiquitin chains in the same fraction was not observed. As for nuclei, increase in multi-ubiquitin chains and concomitant decrease in free ubiquitin were found in the water-soluble extracts after NGF treatment. Lvels of multi-ubiquitin chains did not change in urea-soluble cytoplasmic extracts as well nuclear urea-soluble ones afer NGF treatment. These results indicated that multi-ubiquitination of nuclear proteins is increased during NGF-induced neuronal differentiation of PC12h cells.
  • Morphological study on collateral elimination in the developing corticospinal tract, Ministry of Education, Culture, Sports, Science and Technology, Grants-in-Aid for Scientific Research(一般研究(C)), Ritsuko KANEKO, Collateral elimination (CE) has been known to occur in corticospinal tract (CST) neurons during the postnatal development in rodents. However. how collterals eliminate remains unclear. Although some proteolytic processes might be involved in CE, few works have studied them. In the study, we focussed on ubiquitin and cathepsin-D. The former is a protein with many functions, one of which is related to the proteolysis, and the latter is the major protease in the lysosome. To examine their involvement in CE, we studied the ubiquitin- and cathepsin D-immunoreactive cells in the cervical cord in the pre- and postnatal rats.Wistar-Imamichi rats at various pre- and postnatal stages, as well as adults, were used in this study. After the cervical cord with DRG was removed, it was fixed and embedded in paraffin for LM or embedded in Epon for EM. The sections were stained immunohistochemically, using ubiquitin- or cathepsin D-antiserum. We found numerous cathepsin D-immunoreactive cells in and near CST during the development, suggesting its involvement in CE of CST neurons. Although clear ubiquitin-immunoreactivity could not be observed in CST, we found ubiquitin-immunoreactive nuclei in motoneurons and ganglion cells in the DRG. Ubiquitin might have some other functions than proteolysis in the nucleus of developing neurons.